Detailed information    

experimental Experimentally validated

Overview

Name   comC/comC1   Type   Regulator
Locus tag   SPG_2185 Genome accession   CP001015
Coordinates   2075871..2075996 (-) Length   41 a.a.
NCBI ID   ACF56795.1    Uniprot ID   -
Organism   Streptococcus pneumoniae G54     
Function   binding to ComD; induce autophosphorylation of ComD   
Competence regulation

Function

The comC gene encodes the precursor of the competence-stimulating peptide (CSP), which is processed and secreted by the ComAB transporter. CSP accumulates in the extracellular environment and binds to the histidine kinase ComD, triggering a signaling cascade that activates the expression of competence genes. This process is essential for the induction of the competent state, enabling the bacterium to perform natural transformation. Two major allelic variants of the comC gene have been identified in S. pneumoniae: comC1 and comC2. These variants result in the production of two distinct CSPs, CSP1 and CSP2. Each CSP variant identifies a specific pherotype, and strains carrying one variant are typically unable to respond to the other CSP variant due to receptor specificity.


Genomic Context

Location: 2070871..2080996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPG_2178 - 2071622..2071960 (-) 339 ACF54773.1 conserved hypothetical protein -
  SPG_2179 - 2072045..2072812 (-) 768 ACF56436.1 hypothetical protein -
  SPG_2180 - 2072991..2073533 (+) 543 ACF56205.1 conserved hypothetical protein -
  SPG_2183 comE 2073776..2074528 (-) 753 ACF55408.1 ComE response regulator TCS12 Regulator
  SPG_2184 comD/comD1 2074525..2075850 (-) 1326 ACF56316.1 ComD histidine kinase TCS12 Regulator
  SPG_2185 comC/comC1 2075871..2075996 (-) 126 ACF56795.1 competence-stimulating peptide type 1, CSP1 Regulator
  SPG_2187 - 2076278..2076757 (-) 480 ACF55146.1 conserved hypothetical protein -
  SPG_2188 htrA 2076940..2078121 (+) 1182 ACF55645.1 serine protease Regulator
  SPG_2189 spo0J 2078179..2078937 (+) 759 ACF55136.1 ParB-like partition protein Regulator

Sequence

Protein


Download         Length: 41 a.a.        Molecular weight: 4971.00 Da        Isoelectric Point: 11.0775

>NTDB_id=137 SPG_2185 ACF56795.1 2075871..2075996(-) (comC/comC1) [Streptococcus pneumoniae G54]
MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK

Nucleotide


Download         Length: 126 bp        

>NTDB_id=137 SPG_2185 ACF56795.1 2075871..2075996(-) (comC/comC1) [Streptococcus pneumoniae G54]
ATGAAAAACACAGTTAAATTGGAACAGTTTGTAGCTTTGAAGGAAAAAGACTTACAAAAGATTAAAGGTGGGGAGATGAG
GTTGTCAAAATTCTTCCGTGATTTTATTTTACAAAGAAAAAAGTAA

CSP

This gene is known to encode the precursor of competence stimulating peptide (CSP), which involves in competence development. The mature CSP sequence is displayed as below:

EMRLSKFFRDFILQRKK


Domains

Predicted by InterproScan.

COMC family (ComC)

(1-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comC/comC1 Streptococcus pneumoniae R6

100

100

1
  comC/comC1 Streptococcus pneumoniae D39

100

100

1
  comC/comC1 Streptococcus pneumoniae Rx1

100

100

1
  comC/comC2 Streptococcus pneumoniae A66

80.488

100

0.805
  comC/comC2 Streptococcus pneumoniae TIGR4

80.488

100

0.805
  comC Streptococcus mitis SK321

78.049

100

0.78
  comC Streptococcus mitis NCTC 12261

74.074

67.5

0.5
  comC/blpC Streptococcus mutans UA159

44.444

87.805

0.39

Multiple sequence alignment    



References

[1] Tahmina A Milly et al. (2021) Harnessing Multiple, Nonproteogenic Substitutions to Optimize CSP:ComD Hydrophobic Interactions in Group 1 Streptococcus pneumoniae. Chembiochem : A European Journal of Chemical Biology 22(11):1940-1947. [PMID: 33644965]
[2] Yifang Yang et al. (2019) Exploring the competence stimulating peptide (CSP) N-terminal requirements for effective ComD receptor activation in group1 Streptococcus pneumoniae. Bioorganic Chemistry 89:102987. [PMID: 31132605]
[3] Bimal Koirala et al. (2019) Unveiling the Importance of Amide Protons in CSP:ComD Interactions in Streptococcus pneumoniae. ACS Medicinal Chemistry Letters 10(6):880-886. [PMID: 31223442]
[4] Bimal Koirala et al. (2018) Defining the hydrophobic interactions that drive competence stimulating peptide (CSP)-ComD binding in Streptococcus pneumoniae. Beilstein Journal of Organic Chemistry 14:1769-1777. [PMID: 30112082]
[5] Yifang Yang et al. (2017) Structure-Activity Relationships of the Competence Stimulating Peptides (CSPs) in Streptococcus pneumoniae Reveal Motifs Critical for Intra-group and Cross-group ComD Receptor Activation. ACS Chemical Biology 12(4):1141-1151. [PMID: 28221753]
[6] M Ramirez et al. (1997) Ubiquitous distribution of the competence related genes comA and comC among isolates of Streptococcus pneumoniae. Microbial Drug Resistance (Larchmont, N.Y.) 3(1):39-52. [PMID: 9109095]
[7] G Pozzi et al. (1996) Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone. Journal of Bacteriology 178(20):6087-90. [PMID: 8830714]
[8] L S Håvarstein et al. (1996) Identification of the streptococcal competence-pheromone receptor. Molecular Microbiology 21(4):863-9. [PMID: 8878047]