NTDB

Overview

Name	comYB	Type	Machinery gene
Locus tag	PUW71_RS01115	Genome accession	NZ_CP118053
Coordinates	203770..204792 (+)	Length	340 a.a.
NCBI ID	WP_171021869.1	Uniprot ID	-
Organism	Streptococcus anginosus strain VSI40
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 198770..209792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW71_RS01090 (PUW71_01090)	-	199914..200444 (+)	531	WP_070241503.1	aromatic acid exporter family protein	-
PUW71_RS01095 (PUW71_01095)	-	200521..200880 (+)	360	WP_003070793.1	MerR family transcriptional regulator	-
PUW71_RS01100 (PUW71_01100)	glnA	200918..202264 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW71_RS01105 (PUW71_01105)	-	202452..202817 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW71_RS01110 (PUW71_01110)	comYA	202887..203828 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW71_RS01115 (PUW71_01115)	comYB	203770..204792 (+)	1023	WP_171021869.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW71_RS01120 (PUW71_01120)	comYC	204789..205106 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW71_RS01125 (PUW71_01125)	comYD	205096..205494 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW71_RS01130 (PUW71_01130)	comGE/cglE	205466..205759 (+)	294	WP_195916332.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW71_RS01135 (PUW71_01135)	comGF/cglF	205743..206180 (+)	438	WP_195916333.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW71_RS01140 (PUW71_01140)	comGG	206161..206487 (+)	327	WP_264342122.1	competence type IV pilus minor pilin ComGG	-
PUW71_RS01145 (PUW71_01145)	comYH	206559..207512 (+)	954	WP_195916334.1	class I SAM-dependent methyltransferase	Machinery gene
PUW71_RS01150 (PUW71_01150)	-	207559..208758 (+)	1200	WP_049517013.1	acetate kinase	-

Sequence

Protein

Download Length: 340 a.a. Molecular weight: 39170.30 Da Isoelectric Point: 9.5495

                                >NTDB_id=789576 PUW71_RS01115 WP_171021869.1 203770..204792(+) (comYB) [Streptococcus anginosus strain VSI40]

MQQDISILSRQKRKKLSTVRQKKVVELLNNLFSSGFHLAEIIDFLKRSALLEEVYVDRMKEELSTGKPFSDIMASLGFSD

NVVTQLSLAELHGNLSLSLSKIEEYLENISKIKKKLVEVATYPFILFVFLIFIMLGLRNYLLPQLDRQNIATQIISNLPQ

LFLGSLGVVFVLLAIGFYWFQKSSKIAVFRLLARLPFLGAFIQTYLTAYYAREWGNMIGQGLELSQIFQMMQGQRSAMFQ

EIGKDLEVALQNGQEFSQRVKTYPFFKKELGLMIEYGEVKSKLGCELEVYAQKTWETFFKRVHQAMNVIQPLVFVFVALM

IVLLYAAMLLPIYQNMEVQL

Nucleotide

Download Length: 1023 bp

                                >NTDB_id=789576 PUW71_RS01115 WP_171021869.1 203770..204792(+) (comYB) [Streptococcus anginosus strain VSI40]

ATGCAGCAGGACATATCAATCTTGAGCAGGCAGAAGCGGAAAAAATTATCCACAGTTAGGCAGAAAAAGGTCGTAGAGTT

GCTGAATAATCTTTTTTCTAGTGGCTTTCATTTGGCAGAAATTATCGACTTTTTGAAGCGAAGTGCCTTGTTAGAAGAAG

TCTATGTGGACAGGATGAAAGAGGAGTTGTCAACAGGAAAGCCTTTTTCGGACATTATGGCAAGCTTAGGTTTTTCAGAT

AACGTGGTGACACAGCTTTCGCTGGCAGAATTACACGGAAATCTCTCACTCAGCCTGAGTAAGATTGAGGAATACTTGGA

AAATATCTCAAAAATTAAGAAGAAGTTGGTTGAAGTGGCGACTTATCCCTTTATTTTGTTTGTTTTTTTGATATTCATTA

TGCTGGGATTACGTAATTATTTACTCCCGCAACTCGATCGTCAAAATATAGCAACTCAGATTATCAGTAATTTGCCGCAA

CTTTTTCTGGGTTCATTAGGGGTAGTCTTTGTTTTGTTAGCGATAGGTTTTTATTGGTTTCAAAAAAGCTCAAAAATAGC

AGTATTTCGTTTGTTAGCTCGCCTTCCTTTTTTGGGCGCTTTTATTCAAACCTATTTAACAGCTTATTATGCGAGAGAAT

GGGGGAATATGATTGGTCAAGGTTTAGAACTCAGTCAGATTTTTCAGATGATGCAAGGACAACGCTCTGCTATGTTTCAA

GAAATTGGAAAAGATTTAGAAGTTGCGCTGCAAAATGGTCAGGAATTTTCGCAAAGAGTTAAAACATATCCATTTTTTAA

AAAGGAATTGGGCTTGATGATCGAATACGGTGAAGTAAAATCCAAATTGGGGTGTGAATTAGAAGTTTATGCCCAAAAAA

CATGGGAGACATTTTTTAAACGTGTTCATCAAGCGATGAATGTCATTCAACCACTAGTGTTTGTCTTTGTGGCTTTAATG

ATTGTATTGTTGTATGCAGCCATGTTGCTGCCAATTTATCAAAATATGGAGGTTCAATTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(26-143)

Type II secretion system (T2SS), protein F (T2SSF)

(211-331)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comYB	Streptococcus gordonii str. Challis substr. CH1	70.294	100	0.703
comGB/cglB	Streptococcus mitis SK321	68.657	98.529	0.676
comGB/cglB	Streptococcus mitis NCTC 12261	68.657	98.529	0.676
comGB/cglB	Streptococcus pneumoniae Rx1	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae D39	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae R6	67.761	98.529	0.668
comGB/cglB	Streptococcus pneumoniae TIGR4	67.761	98.529	0.668
comYB	Streptococcus mutans UA159	59.942	100	0.603
comYB	Streptococcus mutans UA140	59.649	100	0.6
comGB	Lactococcus lactis subsp. cremoris KW2	51.039	99.118	0.506

Detailed information NTG0789576