Detailed information NTG0789580

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: PUW71_RS01135
• Genome accession: NZ_CP118053
• Coordinates: 205743..206180 (+)
• Length: 145 a.a.
• NCBI ID: WP_195916333.1
• Uniprot ID: -
• Organism: Streptococcus anginosus strain VSI40
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 200743..211180

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW71_RS01100 (PUW71_01100)	glnA	200918..202264 (+)	1347	WP_024053132.1	type I glutamate--ammonia ligase	-
PUW71_RS01105 (PUW71_01105)	-	202452..202817 (+)	366	WP_024053131.1	DUF1033 family protein	-
PUW71_RS01110 (PUW71_01110)	comYA	202887..203828 (+)	942	WP_024053130.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW71_RS01115 (PUW71_01115)	comYB	203770..204792 (+)	1023	WP_171021869.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW71_RS01120 (PUW71_01120)	comYC	204789..205106 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW71_RS01125 (PUW71_01125)	comYD	205096..205494 (+)	399	WP_022525973.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW71_RS01130 (PUW71_01130)	comGE/cglE	205466..205759 (+)	294	WP_195916332.1	competence type IV pilus minor pilin ComGE	Machinery gene
PUW71_RS01135 (PUW71_01135)	comGF/cglF	205743..206180 (+)	438	WP_195916333.1	competence type IV pilus minor pilin ComGF	Machinery gene
PUW71_RS01140 (PUW71_01140)	comGG	206161..206487 (+)	327	WP_264342122.1	competence type IV pilus minor pilin ComGG	-
PUW71_RS01145 (PUW71_01145)	comYH	206559..207512 (+)	954	WP_195916334.1	class I SAM-dependent methyltransferase	Machinery gene
PUW71_RS01150 (PUW71_01150)	-	207559..208758 (+)	1200	WP_049517013.1	acetate kinase	-
PUW71_RS01155 (PUW71_01155)	glmS	209153..210967 (+)	1815	WP_195916352.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16855.38 Da
• Isoelectric Point: 10.0408

>NTDB_id=789580 PUW71_RS01135 WP_195916333.1 205743..206180(+) (comGF/cglF) [Streptococcus anginosus strain VSI40]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GRAIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIYVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=789580 PUW71_RS01135 WP_195916333.1 205743..206180(+) (comGF/cglF) [Streptococcus anginosus strain VSI40]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGCCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAGGGCAATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTAAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTTATGTTAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	64.789	97.931	0.634
comGF/cglF	Streptococcus pneumoniae TIGR4	64.789	97.931	0.634
comGF/cglF	Streptococcus pneumoniae Rx1	64.789	97.931	0.634
comGF/cglF	Streptococcus pneumoniae D39	64.789	97.931	0.634
comGF/cglF	Streptococcus mitis NCTC 12261	65	96.552	0.628
comGF/cglF	Streptococcus mitis SK321	65	96.552	0.628
comYF	Streptococcus mutans UA159	43.704	93.103	0.407
comYF	Streptococcus mutans UA140	43.704	93.103	0.407
comGF	Lactococcus lactis subsp. cremoris KW2	40.741	93.103	0.379