Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUT72_RS12910 Genome accession   NZ_CP117994
Coordinates   2755050..2756090 (-) Length   346 a.a.
NCBI ID   WP_005381015.1    Uniprot ID   A0A2I3C9Z7
Organism   Vibrio alginolyticus strain 20220413_2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750050..2761090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT72_RS12880 (PUT72_12880) endA 2750195..2750890 (+) 696 WP_017635582.1 deoxyribonuclease I -
  PUT72_RS12885 (PUT72_12885) rsmE 2751004..2751735 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUT72_RS12890 (PUT72_12890) gshB 2751749..2752699 (+) 951 WP_005381021.1 glutathione synthase -
  PUT72_RS12895 (PUT72_12895) - 2752827..2753390 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  PUT72_RS12900 (PUT72_12900) ruvX 2753426..2753851 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  PUT72_RS12905 (PUT72_12905) pilU 2753913..2755019 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUT72_RS12910 (PUT72_12910) pilT 2755050..2756090 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  PUT72_RS12915 (PUT72_12915) - 2756119..2756820 (+) 702 WP_213886906.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUT72_RS12920 (PUT72_12920) proC 2756980..2757798 (+) 819 WP_158174165.1 pyrroline-5-carboxylate reductase -
  PUT72_RS12925 (PUT72_12925) - 2757851..2758408 (+) 558 WP_005381012.1 YggT family protein -
  PUT72_RS12930 (PUT72_12930) yggU 2758408..2758698 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  PUT72_RS12935 (PUT72_12935) - 2758827..2759258 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  PUT72_RS12940 (PUT72_12940) - 2759377..2759979 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38532.02 Da        Isoelectric Point: 6.5039

>NTDB_id=789039 PUT72_RS12910 WP_005381015.1 2755050..2756090(-) (pilT) [Vibrio alginolyticus strain 20220413_2]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFKKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHVLTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVAREEVDSKIGLEVQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=789039 PUT72_RS12910 WP_005381015.1 2755050..2756090(-) (pilT) [Vibrio alginolyticus strain 20220413_2]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCCTCGGATCTACATCTTTCTGCAGGTGTCCCACCAAT
GGTTCGTATTGATGGGGATGTGAGAAAACTAGGTATTCCAGCGTTTACACACCAAGAAGTGCATCGTTTGGTTTTCGAAA
TCATGAACGATGCCCAACGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCTTTTGAGTTGCATAACGTTGGCCGT
TTCCGTGTCAACGCCTTCAACCAATCTCGCGGTTGTGCGGCAGTATTCCGTACCATTCCAAGTAGCATTCCAACTCTGGA
AGAGCTAGAAGCGCCAGAGATCTTCAAAAAAATTGCGAACGCTGAAAAAGGCTTAGTTCTGGTCACTGGCCCAACAGGTT
CAGGTAAATCGACGACCTTAGCAGCCATTGTTGACTACATTAACCGTAATCACAACAAGCACGTACTCACAATTGAAGAT
CCGATTGAATTTGTTCACAACAACAATAAATGTTTGATCAACCAGCGTGAAGTTCACCGTGACACCCATAGTTTCCAAAA
TGCTTTGCGTAGTGCATTGCGCGAAGACCCGGATGTGATTCTAGTCGGTGAGATGCGTGATAAAGAAACCATCAGCCTTG
CCTTAACGGCGGCAGAAACGGGTCACTTAGTTTTCGGGACGTTGCATACCAGCTCGGCGGCAAAAACTATTGACCGTATT
ATCGATGTTTTTCCTGGTAGTGATAAAGATATGGTCCGCTCGATGCTATCTGAGTCTCTTCGTTCGGTTATCGCGCAAAA
GCTTCTGAAGCGTAATGGTGGTGGCCGTATTGCATGCCATGAAATTATGATGGCAACGCCTGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAAACAGGTGCGGCTCACGGCATGCAAACCATGGAGCAAAATGCT
CGTCAGTTAATGGCGCAAGGCATGGTTGCTCGTGAAGAGGTCGATAGTAAGATCGGATTAGAAGTGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.957

99.711

0.867
  pilT Vibrio cholerae strain A1552

86.957

99.711

0.867
  pilT Acinetobacter baumannii D1279779

72.121

95.376

0.688
  pilT Acinetobacter baumannii strain A118

72.121

95.376

0.688
  pilT Acinetobacter nosocomialis M2

71.818

95.376

0.685
  pilT Acinetobacter baylyi ADP1

71.429

95.087

0.679
  pilT Pseudomonas aeruginosa PAK

69.139

97.399

0.673
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.399

0.671
  pilT Legionella pneumophila strain Lp02

67.674

95.665

0.647
  pilT Legionella pneumophila strain ERS1305867

67.674

95.665

0.647
  pilT Neisseria meningitidis 8013

65.766

96.243

0.633
  pilT Neisseria gonorrhoeae MS11

65.465

96.243

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.83

91.908

0.486
  pilU Vibrio cholerae strain A1552

41.317

96.532

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361