Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUT72_RS12905 Genome accession   NZ_CP117994
Coordinates   2753913..2755019 (-) Length   368 a.a.
NCBI ID   WP_005381016.1    Uniprot ID   A0A2I3C9Z5
Organism   Vibrio alginolyticus strain 20220413_2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748913..2760019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUT72_RS12875 (PUT72_12875) - 2749547..2750044 (+) 498 WP_005381025.1 SprT family zinc-dependent metalloprotease -
  PUT72_RS12880 (PUT72_12880) endA 2750195..2750890 (+) 696 WP_017635582.1 deoxyribonuclease I -
  PUT72_RS12885 (PUT72_12885) rsmE 2751004..2751735 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUT72_RS12890 (PUT72_12890) gshB 2751749..2752699 (+) 951 WP_005381021.1 glutathione synthase -
  PUT72_RS12895 (PUT72_12895) - 2752827..2753390 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  PUT72_RS12900 (PUT72_12900) ruvX 2753426..2753851 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  PUT72_RS12905 (PUT72_12905) pilU 2753913..2755019 (-) 1107 WP_005381016.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PUT72_RS12910 (PUT72_12910) pilT 2755050..2756090 (-) 1041 WP_005381015.1 type IV pilus twitching motility protein PilT Machinery gene
  PUT72_RS12915 (PUT72_12915) - 2756119..2756820 (+) 702 WP_213886906.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUT72_RS12920 (PUT72_12920) proC 2756980..2757798 (+) 819 WP_158174165.1 pyrroline-5-carboxylate reductase -
  PUT72_RS12925 (PUT72_12925) - 2757851..2758408 (+) 558 WP_005381012.1 YggT family protein -
  PUT72_RS12930 (PUT72_12930) yggU 2758408..2758698 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  PUT72_RS12935 (PUT72_12935) - 2758827..2759258 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  PUT72_RS12940 (PUT72_12940) - 2759377..2759979 (+) 603 WP_005381009.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41254.36 Da        Isoelectric Point: 6.5943

>NTDB_id=789038 PUT72_RS12905 WP_005381016.1 2753913..2755019(-) (pilU) [Vibrio alginolyticus strain 20220413_2]
MDLDKFLEGMLALKASDLYITVGAPILFRVDGELRPQGEKLTESDVAALLDSAMEPDRRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=789038 PUT72_RS12905 WP_005381016.1 2753913..2755019(-) (pilU) [Vibrio alginolyticus strain 20220413_2]
ATGGATCTAGATAAATTTCTCGAAGGCATGCTGGCGCTGAAAGCGTCGGATCTTTACATCACGGTTGGAGCGCCAATTCT
ATTTCGTGTGGATGGCGAATTGCGTCCGCAAGGGGAAAAACTTACCGAGAGTGATGTGGCTGCATTACTCGATAGCGCTA
TGGAACCAGATCGGCGTCAGGAATTTCGTAAAAGTCGCGAGTCGAATTTTGCCATTGTGAGAGATTGTGGTCGCTTCCGT
GTGAGTGCCTTTTTTCAACGCGAGTTACCAGGCGCAGTGATTCGTCGTATTGAAACCAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAGTTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGTGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAGTACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAACAATGCAAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTTTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCCTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCAAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I3C9Z5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.231

88.315

0.399
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.692

89.946

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.761

0.375
  pilT Vibrio cholerae strain A1552

41.317

90.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361