Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PT256_RS04975 Genome accession   NZ_CP117817
Coordinates   1044402..1045466 (+) Length   354 a.a.
NCBI ID   WP_136629019.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5573     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1039402..1050466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT256_RS04965 (PT256_04965) - 1040444..1042828 (-) 2385 WP_027226227.1 penicillin-binding protein 1A -
  PT256_RS04970 (PT256_04970) - 1042978..1043985 (-) 1008 WP_013101264.1 hypothetical protein -
  PT256_RS04975 (PT256_04975) pilM 1044402..1045466 (+) 1065 WP_136629019.1 type IV pilus assembly protein PilM Machinery gene
  PT256_RS04980 (PT256_04980) pilN 1045470..1046018 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  PT256_RS04985 (PT256_04985) pilO 1046032..1046631 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  PT256_RS04990 (PT256_04990) pilP 1046628..1047212 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  PT256_RS04995 (PT256_04995) pilQ 1047216..1049312 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  PT256_RS05000 (PT256_05000) aroK 1049759..1050286 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38787.80 Da        Isoelectric Point: 5.0367

>NTDB_id=787680 PT256_RS04975 WP_136629019.1 1044402..1045466(+) (pilM) [Legionella pneumophila strain D5573]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=787680 PT256_RS04975 WP_136629019.1 1044402..1045466(+) (pilM) [Legionella pneumophila strain D5573]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGTTTTGTAGACCATATTTTATTGG
CAGGAGGAATGGCCAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAACCCG
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAATGATGCGCCTACACTAATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.718

100

0.997
  comM Acinetobacter nosocomialis M2

42.655

100

0.427
  pilM Acinetobacter baumannii D1279779

42.373

100

0.424
  comM Acinetobacter baylyi ADP1

40.96

100

0.41