Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   PT256_RS04980 Genome accession   NZ_CP117817
Coordinates   1045470..1046018 (+) Length   182 a.a.
NCBI ID   WP_013101266.1    Uniprot ID   A0A378K2G2
Organism   Legionella pneumophila strain D5573     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040470..1051018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT256_RS04970 (PT256_04970) - 1042978..1043985 (-) 1008 WP_013101264.1 hypothetical protein -
  PT256_RS04975 (PT256_04975) pilM 1044402..1045466 (+) 1065 WP_136629019.1 type IV pilus assembly protein PilM Machinery gene
  PT256_RS04980 (PT256_04980) pilN 1045470..1046018 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  PT256_RS04985 (PT256_04985) pilO 1046032..1046631 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  PT256_RS04990 (PT256_04990) pilP 1046628..1047212 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  PT256_RS04995 (PT256_04995) pilQ 1047216..1049312 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  PT256_RS05000 (PT256_05000) aroK 1049759..1050286 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21336.10 Da        Isoelectric Point: 10.1636

>NTDB_id=787681 PT256_RS04980 WP_013101266.1 1045470..1046018(+) (pilN) [Legionella pneumophila strain D5573]
MTEINLLPWREQRREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLINNQMNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDIVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
DVQKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=787681 PT256_RS04980 WP_013101266.1 1045470..1046018(+) (pilN) [Legionella pneumophila strain D5573]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAGACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGGATTATCATTGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTCTCTCATTTAATAAATAATCAAATGAACCGAA
ACCAGATTTTGCAAAAAGAAATTACTGCTTTAGATGAGCAGATCAAGGAGATTAAGGCACTAAAACAAATCAGGGAAGGT
TTGATTTCGAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGGACTTTAATGGTTCACTTATTTGATGAATTAATAAA
AATTACTCCTCCAGGCGTTTACATAACCAAGATGCAGAGAGAACACGATATAGTAACACTCTGGGGGTATTCTGAGTCGA
ATACAAGTATTTCTATTTTAATGAGAAATATAGAGGCTAATGTCTGGATACAGAATCCTGTCCTAACTGAAATTAAAAAA
GATGTTCAAAAAAAACAGTCTCCAAATAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K2G2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

96.703

100

0.967