Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSH91_RS16565 Genome accession   NZ_CP117465
Coordinates   3705769..3706887 (-) Length   372 a.a.
NCBI ID   WP_107322662.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1154     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3700769..3711887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH91_RS16550 (PSH91_16545) - 3701282..3702673 (+) 1392 WP_047227845.1 GntP family permease -
  PSH91_RS16555 (PSH91_16550) hbdH 3702682..3703458 (+) 777 WP_076384048.1 3-hydroxybutyrate dehydrogenase -
  PSH91_RS16560 (PSH91_16555) - 3703662..3705617 (+) 1956 WP_305466236.1 acetoacetate--CoA ligase -
  PSH91_RS16565 (PSH91_16560) pilU 3705769..3706887 (-) 1119 WP_107322662.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSH91_RS16570 (PSH91_16565) - 3707022..3709559 (+) 2538 WP_107322661.1 PAS domain-containing protein -
  PSH91_RS16575 (PSH91_16570) - 3709608..3709883 (+) 276 WP_076384045.1 peptidylprolyl isomerase -
  PSH91_RS16580 (PSH91_16575) - 3709921..3710844 (-) 924 WP_305466240.1 sugar kinase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41274.19 Da        Isoelectric Point: 6.1904

>NTDB_id=785840 PSH91_RS16565 WP_107322662.1 3705769..3706887(-) (pilU) [Pseudomonas sp. FP1154]
MEIDPLLRILASQDGSDLYMSTGAPPCARFEGVLKPLSNQPFKVGDIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPNVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNEFGGLKGIMEKSEELGMQTFDHAL
YNLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAAVPPADPAAGEWGLMD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=785840 PSH91_RS16565 WP_107322662.1 3705769..3706887(-) (pilU) [Pseudomonas sp. FP1154]
ATGGAAATCGATCCCTTGTTACGCATCCTGGCGAGCCAGGATGGCTCCGACCTCTATATGTCCACCGGTGCGCCGCCGTG
CGCGCGTTTCGAGGGGGTGCTCAAACCCTTGAGCAACCAGCCCTTCAAGGTGGGCGACATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGGCTTGAGTTCGATCGAGAACTGGAAATGAACCTGGCTATCTCCCTGGCTGGGGTCGGGCGG
TTTCGCGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTCGACATCCCTCGGTTCGA
GGACCTGAAACTGCCAAATGTGTTGCTGGACACCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGCGCGACAGGGT
CGGGCAAGTCGACTTCCCTGGCGGCGCTGATCGATTACCGTAATCGCAACAGCAGCGGCCATATCATCACCATTGAAGAT
CCGGTGGAGTATATCCATCGACACAAGAAATCGATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGCTTTCATGC
CGCCCTGAAGAACACCTTGCGCCAGGCACCAGACGTGGTACTGATCGGCGAAATTCGTGATCGCGAAACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGGCACCTGGTGATCTCCACCCTGCATGCTCACAATGCCAACCAGGCGCTGGACCGGGTG
ATCAATTTCTTCCCGGAAGAACGCCGGCCGCAGCTGCTCAATGATCTGGGCAACAACCTCAAGGCGTTCGTGTCGCAACG
CCTGGTGCGCACTCGCACCGGCCAGCGCCGGGCCGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GCCGCAATGAGTTCGGCGGGCTCAAGGGCATCATGGAAAAGTCAGAAGAACTCGGGATGCAAACCTTCGATCACGCCCTG
TACAACCTGGTGGTGGAGGGTGCTATCGATGAGGAGGAAGCCCTGAAAAACGCCGATTCGGCGAACAACCTGCGGTTGCG
CCTGAAGTTGCATGCCGAGTCGGGGGCAGCGGTGCCCCCGGCCGATCCGGCGGCGGGGGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.62

95.43

0.54
  pilU Acinetobacter baylyi ADP1

51.685

95.699

0.495
  pilU Vibrio cholerae strain A1552

51.714

94.086

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.235

100

0.384
  pilT Pseudomonas aeruginosa PAK

39.244

92.473

0.363
  pilT Pseudomonas stutzeri DSM 10701

38.953

92.473

0.36