Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH91_RS04215 Genome accession   NZ_CP117465
Coordinates   946823..948523 (-) Length   566 a.a.
NCBI ID   WP_047229859.1    Uniprot ID   A0AA96XX08
Organism   Pseudomonas sp. FP1154     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 941823..953523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH91_RS04180 (PSH91_04180) - 942130..942756 (-) 627 WP_305467387.1 DUF1780 domain-containing protein -
  PSH91_RS04185 (PSH91_04185) - 942902..943588 (+) 687 WP_047229865.1 energy-coupling factor ABC transporter permease -
  PSH91_RS04190 (PSH91_04190) - 943652..943867 (+) 216 WP_092402997.1 hypothetical protein -
  PSH91_RS04195 (PSH91_04195) yacG 943900..944100 (-) 201 WP_047229863.1 DNA gyrase inhibitor YacG -
  PSH91_RS04200 (PSH91_04200) coaE 944097..944720 (-) 624 WP_047229862.1 dephospho-CoA kinase -
  PSH91_RS04205 (PSH91_04205) pilD 944732..945601 (-) 870 WP_047229861.1 A24 family peptidase Machinery gene
  PSH91_RS04210 (PSH91_04210) pilC 945603..946820 (-) 1218 WP_076386354.1 type II secretion system F family protein Machinery gene
  PSH91_RS04215 (PSH91_04215) pilB 946823..948523 (-) 1701 WP_047229859.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH91_RS04220 (PSH91_04220) pilA 948749..949168 (+) 420 WP_047229858.1 pilin Machinery gene
  PSH91_RS04225 (PSH91_04225) - 949449..949802 (+) 354 WP_047229857.1 BON domain-containing protein -
  PSH91_RS04230 (PSH91_04230) - 950126..950422 (-) 297 WP_047229856.1 DUF2845 domain-containing protein -
  PSH91_RS04235 (PSH91_04235) - 950424..953357 (-) 2934 WP_305467392.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62747.81 Da        Isoelectric Point: 5.4229

>NTDB_id=785832 PSH91_RS04215 WP_047229859.1 946823..948523(-) (pilB) [Pseudomonas sp. FP1154]
MNDIALSGLAKQLVLAELITEQSAQQAYQQAQSSRMPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESTSTGLEGMGDVDLDG
LDIESIDDHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKNKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKELYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLIRLHNMGIPGFNIATAVHLIIAQRLARKLCPHCKRAIEIPEETLLKEGFPR
ERIGSFTIYEPVGCEQCNNGYKGRVGVYEVVRNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785832 PSH91_RS04215 WP_047229859.1 946823..948523(-) (pilB) [Pseudomonas sp. FP1154]
ATGAATGACATCGCCTTAAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAACTGATCACCGAGCAAAGTGCGCAGCAGGC
GTACCAACAAGCCCAAAGCAGTCGCATGCCCCTGGTCAGTTACCTGGTGCAGAACAAACTGGTCCAGAGTCGCCAGGTGG
CGGAAATTGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAACCCAACCCACCGGCCTT
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATTCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTTTTCGAATCCACCAGCACCGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGACATCGAGTCGATCGATGATCACAAGCAAGACTCCATCGCCGGCCAGGACACCGACGATGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCCGACCTGCACTTTGAACCCTATGAAAAAATCTACCGGG
TGCGGGTGCGTACCGACGGCATGCTGCGAGAGGTGGCCCGCCCGCCGATCCAGTTGGCCACCCGCATCGCCGCGCGCCTG
AAGGTCATGGCCAGCCTTGATATTTCCGAGCGGCGCAAACCCCAGGACGGACGCCTGAAAATGCGCCTGTCGAAAAACAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACGCTCTGGGGTGAAAAAGTGGTGATCCGGATCCTCGACCCCTCCAGCG
CGCAGATGGGTATCGATGCCCTGGGCTACGAGCCGGACCAGAAAGAGCTGTACATGGCGGCGCTCAAACAACCACAAGGG
TTGATCCTGGTGACCGGGCCCACCGGCTCTGGCAAAACCGTGTCGCTCTATACCGGGCTCAATATCCTCAACACTGTGGA
CATCAATATTTCCACCGCTGAGGATCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAGG
GTCTGGATTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGACACATGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACGGCGGTCCACCTGATCATCGCCCAGCGAC
TGGCGCGCAAGCTGTGCCCGCATTGCAAAAGGGCCATCGAGATTCCCGAGGAAACCCTGCTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCTCGTTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACAACGGCTACAAGGGGCGCGTGGGGGT
TTACGAAGTGGTCAGGAATACGCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGTTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTTAATGACCTGCGCGCCTCGGGACTGCTCAAAGTGATGCAAGGCATCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.304

100

0.583
  pilB Acinetobacter baumannii D1279779

56.738

99.647

0.565
  pilB Legionella pneumophila strain ERS1305867

55.458

100

0.557
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilF Neisseria gonorrhoeae MS11

48.668

99.47

0.484
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.088

96.82

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.833

99.293

0.366