Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH69_RS25505 Genome accession   NZ_CP117461
Coordinates   5687177..5688877 (+) Length   566 a.a.
NCBI ID   WP_305410175.1    Uniprot ID   -
Organism   Pseudomonas sp. FP1740     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5682177..5693877
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH69_RS25485 (PSH69_25475) - 5685127..5685423 (+) 297 WP_305410172.1 DUF2845 domain-containing protein -
  PSH69_RS25490 (PSH69_25480) - 5685597..5685953 (-) 357 WP_305410173.1 BON domain-containing protein -
  PSH69_RS25495 (PSH69_25485) - 5686236..5686406 (-) 171 Protein_5037 IS110 family transposase -
  PSH69_RS25500 (PSH69_25490) - 5686531..5686950 (-) 420 WP_305410174.1 pilin -
  PSH69_RS25505 (PSH69_25495) pilB 5687177..5688877 (+) 1701 WP_305410175.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH69_RS25510 (PSH69_25500) pilC 5688880..5690097 (+) 1218 WP_305410176.1 type II secretion system F family protein Machinery gene
  PSH69_RS25515 (PSH69_25505) pilD 5690099..5690971 (+) 873 WP_096479536.1 A24 family peptidase Machinery gene
  PSH69_RS25520 (PSH69_25510) coaE 5690968..5691591 (+) 624 WP_026286732.1 dephospho-CoA kinase -
  PSH69_RS25525 (PSH69_25515) yacG 5691588..5691788 (+) 201 WP_305410177.1 DNA gyrase inhibitor YacG -
  PSH69_RS25530 (PSH69_25520) - 5691798..5692013 (-) 216 WP_007938959.1 hypothetical protein -
  PSH69_RS25535 (PSH69_25525) - 5692077..5692766 (-) 690 WP_305410178.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62887.95 Da        Isoelectric Point: 5.5522

>NTDB_id=785731 PSH69_RS25505 WP_305410175.1 5687177..5688877(+) (pilB) [Pseudomonas sp. FP1740]
MNDIALSGLAKQLVLAELITDQSAQKAYQQAQRDRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLDCLEKDTQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPSNQQVINDIQFSTGLNTEAILVEEDKLSDAIEKFFDSHATGLEEMADVDLDG
LDIESVDDQKQDAIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKSYRVRMRTDGILHEVAKPPTQLANRIASRL
KIMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGVNQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCNQCNAGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785731 PSH69_RS25505 WP_305410175.1 5687177..5688877(+) (pilB) [Pseudomonas sp. FP1740]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAGCTGATCACTGATCAAAGTGCGCAAAAGGC
TTATCAGCAAGCCCAACGCGATCGAGTCTCACTGGTCAGTTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCGACTGCCTGGAAAAAGACACCCAACCCAAGGGGCTG
GTCAGCGAAAAACTGATCCGCCAGCACCACGCCCTACCCCTCTGGCGACGCGGCAACAAACTGTTCGTGGGGGTTTCCGA
TCCGAGCAATCAACAGGTCATCAACGATATCCAGTTCAGTACCGGGCTGAACACCGAAGCCATCCTGGTCGAAGAAGACA
AGCTCAGCGACGCCATTGAAAAATTCTTCGACAGCCACGCCACCGGCCTGGAAGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGTCGACGACCAGAAACAGGACGCCATCGCCGGGCAGGACACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGGTCTTCCGACCTGCATTTCGAACCCTACGAGAAATCCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAAGTCGCCAAGCCACCCACGCAACTGGCCAACCGCATTGCTTCGCGACTC
AAAATCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTCGGCTATGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCACAGGGG
ATGATCCTGGTGACCGGGCCCACCGGTTCAGGCAAGACCGTGTCGCTCTATACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAGGGCGTCAACCAGGTCAACGTCAATCCCAGGCAGG
GAATGGATTTCGCCCAGGCGTTGCGCTCGTTTCTGCGCCAAGACCCCGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACTGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGCTTCAACATTGCGACCTCGGTCAGTCTGATCATTGCCCAGCGCC
TGGCGCGCAAGTTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGCTGCAATCAATGCAACGCCGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTAAAGAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAGATCGACCTTCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACGTCGGGCTTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AATCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Acinetobacter baylyi ADP1

57.672

100

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.18

98.233

0.493
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilF Thermus thermophilus HB27

36.396

100

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36