Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH92_RS02250 Genome accession   NZ_CP117451
Coordinates   510661..512748 (+) Length   695 a.a.
NCBI ID   WP_305469355.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505661..517748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH92_RS02230 (PSH92_02230) comM 507870..508934 (+) 1065 WP_122565300.1 pilus assembly protein PilM Machinery gene
  PSH92_RS02235 (PSH92_02235) - 508934..509500 (+) 567 WP_122565301.1 PilN domain-containing protein -
  PSH92_RS02240 (PSH92_02240) pilO 509497..510120 (+) 624 WP_122565302.1 type 4a pilus biogenesis protein PilO -
  PSH92_RS02245 (PSH92_02245) - 510117..510647 (+) 531 WP_305469354.1 pilus assembly protein PilP -
  PSH92_RS02250 (PSH92_02250) pilQ 510661..512748 (+) 2088 WP_305469355.1 type IV pilus secretin PilQ Machinery gene
  PSH92_RS02255 (PSH92_02255) aroK 512753..513271 (+) 519 WP_122565305.1 shikimate kinase AroK -
  PSH92_RS02260 (PSH92_02260) aroB 513420..514520 (+) 1101 WP_305469357.1 3-dehydroquinate synthase -
  PSH92_RS02265 (PSH92_02265) - 514531..516120 (+) 1590 WP_305469358.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75150.32 Da        Isoelectric Point: 5.5858

>NTDB_id=785436 PSH92_RS02250 WP_305469355.1 510661..512748(+) (pilQ) [Pseudomonas beijingensis strain FP2034]
MNRIFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLTNRRHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYETRVEGNNLFVVVGKGQTPKNTASKPSAQAPRPAVASAAPARASAPAGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGTSTTIGTPGSTGTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=785436 PSH92_RS02250 WP_305469355.1 510661..512748(+) (pilQ) [Pseudomonas beijingensis strain FP2034]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTTGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCAACACCCCGGG
GCTACACCACCGAACAGCCGGCCCGGATTGCCCTGGACCTGCCAGGCGTGACCAATCAACTGACCAACAGGCGTCACGAC
CTGGGCGGGGGCAATGCGCGCAGCGCCACCGTGGTCGAGGCGGGGGATCGCACCCGGTTGATCATCGGCCTGACCCAACT
GGCGCCGTATGAAACCCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTTGGCAAGGGCCAGACCCCGAAGAATACTGCTT
CGAAGCCCTCTGCCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCCGCGCGGGCCAGTGCGCCGGCGGGCAAGGCG
ATTCGTGGGGTGGATTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGATTTGTCCGATCCATCCATCGCCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAATTGCCCGAGCCACTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCTGGCAGCGACCGGGCGACCATCAGCATCGAGCCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTGGACGACTT
GCAAAAACGCAATGCCGACCGCGCCGCCTACAGCGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAAGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACAGTGCAGGGTGGCATCACCCTGCGGTTG
CAGAACGTGCCTTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAACGTGCT
GCTGGTGGCCCCGGCCGATGAAATCGCGGCGCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGGCGC
CGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGGCGGACATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCC
GAGGCGAAAGCCGACGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
GCTCGACGAACTGCGCCGGATCGTGGCACAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGGTCGATACAGAACAAGGGCAACTGGAACTCCTCGGGG
GTGACCACTGGCACCTCCACGACCATCGGCACGCCGGGCAGTACCGGCACCAACCAGCCGTTCGTCGACCTGGGTGCCTC
GGGTAATACCTCGGGGATCGGCATTGCGTTCATCACCGACAACGTGTTGCTGGACCTTGAGCTGACTGCCATGGAAAAAA
CCGGCAACGGCGAAATTGTCTCCCAGCCCAAGGTGGTGACATCTGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAG
ATCCCTTATCAGGAAGCCAGTTCCAGCGGCGCCACCTCGGTGTCGTTCAAGGAGGCCTCGCTATCGCTGGAGGTGACCCC
GCAAATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCTGATTACCTGAACAAGGTGCAGG
AAGTGCCACCGATCAAGAAAAACGAAGTCAACGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.751
  pilQ Acinetobacter baumannii strain A118

38.904

100

0.419
  pilQ Acinetobacter baumannii D1279779

38.904

100

0.419
  comQ Acinetobacter baylyi ADP1

41.81

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.144

97.698

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.018

97.266

0.37