Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSH57_RS10440 Genome accession   NZ_CP117449
Coordinates   2343383..2344501 (+) Length   372 a.a.
NCBI ID   WP_305389357.1    Uniprot ID   -
Organism   Pseudomonas hefeiensis strain FP205     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2338383..2349501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH57_RS10425 (PSH57_10425) - 2339328..2340251 (+) 924 WP_305389353.1 sugar kinase -
  PSH57_RS10430 (PSH57_10430) - 2340387..2340662 (-) 276 WP_019690952.1 peptidylprolyl isomerase -
  PSH57_RS10435 (PSH57_10435) - 2340711..2343248 (-) 2538 WP_305389355.1 PAS domain-containing protein -
  PSH57_RS10440 (PSH57_10440) pilU 2343383..2344501 (+) 1119 WP_305389357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSH57_RS10445 (PSH57_10445) - 2344757..2346712 (-) 1956 WP_305389359.1 acetoacetate--CoA ligase -
  PSH57_RS10450 (PSH57_10450) hbdH 2346915..2347691 (-) 777 WP_305389360.1 3-hydroxybutyrate dehydrogenase -
  PSH57_RS10455 (PSH57_10455) - 2347699..2349090 (-) 1392 WP_305389361.1 GntP family permease -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41341.31 Da        Isoelectric Point: 6.0196

>NTDB_id=785387 PSH57_RS10440 WP_305389357.1 2343383..2344501(+) (pilU) [Pseudomonas hefeiensis strain FP205]
MEIDPLLRILASQDGSDLYLSTGAPPCARFEGLLKPLSNQPFKVGEIAGLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPRFEDLKLPNVLLDIIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNEFGELKGIMEKSEELGMQTFDHAL
YSLVVEGAIDEEEALKNADSANNLRLRLKLHAESGAAVPPADPAAGEWGLMD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=785387 PSH57_RS10440 WP_305389357.1 2343383..2344501(+) (pilU) [Pseudomonas hefeiensis strain FP205]
ATGGAAATCGATCCCTTGTTACGCATCCTGGCGAGCCAGGATGGCTCCGACCTCTACCTGTCCACCGGCGCGCCGCCATG
CGCGCGTTTCGAGGGGCTGCTCAAACCCTTGAGCAACCAGCCGTTCAAGGTTGGCGAGATCGCCGGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGGCTTGAGTTCGATCGGGAACTGGAAATGAACCTGGCCATCTCCCTGGCCGGGGTCGGGCGG
TTCCGCGTCAATATCTTCAAGCAACGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTCGACATCCCGCGGTTCGA
GGACCTGAAACTGCCGAATGTGTTGCTGGACATCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGCGCGACAGGGT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGTAATCGCAACAGCAGCGGCCATATCATCACCATCGAAGAT
CCGGTGGAGTATATCCATCGACACAAGAAATCGATCATCAATCAACGCGAAGTCGGTGTCGACACCCGCAGCTTTCATGC
CGCTTTGAAGAACACCTTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATTCGTGATCGCGAAACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGGCATCTGGTGATCTCCACCCTGCATGCTCACAATGCCAATCAGGCGTTGGACCGGGTG
ATCAATTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAATGATCTGGGCAACAACCTCAAGGCGTTCGTGTCGCAACG
CCTTGTGCGCACTCGCACCGGCCAGCGCCGGGCCGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATTC
GCCGCAATGAGTTTGGCGAGCTCAAGGGCATCATGGAAAAGTCAGAAGAACTCGGGATGCAAACCTTCGACCACGCCCTG
TACAGCCTGGTGGTGGAGGGGGCTATCGATGAGGAGGAAGCCCTGAAAAACGCCGACTCGGCGAACAACCTGCGGTTGCG
GCTGAAGTTGCATGCCGAATCGGGGGCGGCGGTGCCCCCGGCCGATCCGGCGGCGGGTGAGTGGGGGTTGATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.62

95.43

0.54
  pilU Acinetobacter baylyi ADP1

51.541

95.968

0.495
  pilU Vibrio cholerae strain A1552

51.714

94.086

0.487
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

98.387

0.387
  pilT Pseudomonas aeruginosa PAK

39.535

92.473

0.366
  pilT Pseudomonas stutzeri DSM 10701

39.535

92.473

0.366