Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSH83_RS24460 Genome accession   NZ_CP117443
Coordinates   5573777..5575477 (+) Length   566 a.a.
NCBI ID   WP_305491120.1    Uniprot ID   -
Organism   Pseudomonas beijingensis strain FP2262     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5568777..5580477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH83_RS24440 (PSH83_24440) - 5568863..5571808 (+) 2946 WP_305492455.1 DUF748 domain-containing protein -
  PSH83_RS24445 (PSH83_24445) - 5571810..5572106 (+) 297 WP_305491118.1 DUF2845 domain-containing protein -
  PSH83_RS24450 (PSH83_24450) - 5572442..5572795 (-) 354 WP_003205664.1 BON domain-containing protein -
  PSH83_RS24455 (PSH83_24455) - 5573124..5573552 (-) 429 WP_305491119.1 pilin -
  PSH83_RS24460 (PSH83_24460) pilB 5573777..5575477 (+) 1701 WP_305491120.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH83_RS24465 (PSH83_24465) pilC 5575480..5576697 (+) 1218 WP_122564917.1 type II secretion system F family protein Machinery gene
  PSH83_RS24470 (PSH83_24470) pilD 5576699..5577568 (+) 870 WP_305491121.1 A24 family peptidase Machinery gene
  PSH83_RS24475 (PSH83_24475) coaE 5577581..5578204 (+) 624 WP_122564919.1 dephospho-CoA kinase -
  PSH83_RS24480 (PSH83_24480) yacG 5578201..5578407 (+) 207 WP_305491122.1 DNA gyrase inhibitor YacG -
  PSH83_RS24485 (PSH83_24485) - 5578404..5578619 (-) 216 WP_053125334.1 hypothetical protein -
  PSH83_RS24490 (PSH83_24490) - 5578683..5579369 (-) 687 WP_305468537.1 energy-coupling factor ABC transporter permease -
  PSH83_RS24495 (PSH83_24495) - 5579529..5580155 (+) 627 WP_025215531.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62620.67 Da        Isoelectric Point: 5.4307

>NTDB_id=785240 PSH83_RS24460 WP_305491120.1 5573777..5575477(+) (pilB) [Pseudomonas beijingensis strain FP2262]
MNDIALSGLTKQLVLAELITEQNAQQAYQQAQRSRIPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKEAQPTGL
VSEKLVRQHHALPLLRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESTSTGLEGMGDADLDG
LDIESIDDTKQDSIGGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEVPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=785240 PSH83_RS24460 WP_305491120.1 5573777..5575477(+) (pilB) [Pseudomonas beijingensis strain FP2262]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGATCACCGAGCAAAATGCGCAACAGGC
GTATCAACAGGCTCAACGCAGCCGCATCCCCTTGGTCAGCTACCTGGTGCAGAACAAACTGGTCCAGAGCCGTCAGGTCG
CGGAAATCGCCTCGGAGCATTTCGGCGTCGCCCTGCTGGATCTCAACAGCCTGGACAAGGAAGCCCAGCCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCATCACGCCCTGCCGCTGTTGCGGCGTGGCAACAAACTGTTCGTGGGCATCTCCGA
CCCCACCAACCACCAGGCCATCAACGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AACTCAGCGACGCCATCGAGAAGTTCTTCGAATCCACGAGTACAGGCCTGGAAGGCATGGGCGATGCCGACCTCGACGGC
CTGGACATCGAATCGATCGACGACACCAAGCAGGATTCCATCGGCGGCCAGGACACCGACGACGCGCCCGTGGTGCGTTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAAAAAATCTACCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTGGCCCGGCCGCCGATCCAACTGGCGACCCGTATCGCCGCCCGCCTC
AAGGTCATGGCCAGCCTGGATATTTCCGAACGGCGCAAACCCCAGGACGGCCGGTTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAGGTGGTGATCCGGATCCTCGATCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGATCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCGCAAGGG
CTGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAATATCCTCAACACCGTGGA
CATCAATATTTCCACCGCTGAGGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCGGCCGA
AACCCTGATCCGCTTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAGGCCATTGAGGTTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGCTTCAATGACCTGCGCACATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.187

100

0.592
  pilB Acinetobacter baumannii D1279779

56.915

99.647

0.567
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

49.18

96.996

0.477