Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IL310_RS05300 Genome accession   NZ_CP117409
Coordinates   605234..605881 (+) Length   215 a.a.
NCBI ID   WP_201248262.1    Uniprot ID   -
Organism   Lactococcus lactis strain LB7     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 600234..610881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL310_RS05275 (IL310_05280) - 600363..601541 (+) 1179 WP_012898345.1 SLC13 family permease -
  IL310_RS05280 (IL310_05285) - 601715..602278 (+) 564 WP_004255233.1 GNAT family N-acetyltransferase -
  IL310_RS05285 (IL310_05290) - 602382..602780 (+) 399 WP_004255236.1 hypothetical protein -
  IL310_RS05290 (IL310_05295) - 602885..603817 (+) 933 WP_201248264.1 ABC transporter ATP-binding protein -
  IL310_RS05295 (IL310_05300) - 603814..605175 (+) 1362 WP_201248263.1 ABC transporter permease -
  IL310_RS05300 (IL310_05305) comEA 605234..605881 (+) 648 WP_201248262.1 ComEA family DNA-binding protein Machinery gene
  IL310_RS05305 (IL310_05310) comEC 605862..608072 (+) 2211 WP_201248261.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IL310_RS05310 (IL310_05315) - 608357..609133 (+) 777 WP_058220836.1 alpha/beta hydrolase family protein -
  IL310_RS05315 (IL310_05320) - 609317..609532 (+) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  IL310_RS05320 (IL310_05325) atpB 609577..610290 (+) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  IL310_RS05325 (IL310_05330) atpF 610305..610811 (+) 507 WP_010906128.1 F0F1 ATP synthase subunit B -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23604.77 Da        Isoelectric Point: 4.8659

>NTDB_id=784632 IL310_RS05300 WP_201248262.1 605234..605881(+) (comEA) [Lactococcus lactis strain LB7]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSAENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=784632 IL310_RS05300 WP_201248262.1 605234..605881(+) (comEA) [Lactococcus lactis strain LB7]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCGCAG
AAAATACTGATAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

75.688

100

0.767
  comEA/celA/cilE Streptococcus mitis SK321

44.907

100

0.451
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

43.578

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae Rx1

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae D39

41.553

100

0.423
  comEA/celA/cilE Streptococcus pneumoniae R6

41.553

100

0.423
  comEA/celA/cilE Streptococcus mitis NCTC 12261

41.743

100

0.423
  comEA Latilactobacillus sakei subsp. sakei 23K

36.564

100

0.386