Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9D40_RS06340 Genome accession   NZ_CP117196
Coordinates   1335456..1335890 (-) Length   144 a.a.
NCBI ID   WP_005741096.1    Uniprot ID   -
Organism   Pasteurella multocida strain P2237     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1330456..1340890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9D40_RS06310 (M9D40_006310) - 1331020..1331289 (-) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  M9D40_RS06315 (M9D40_006315) yacG 1331289..1331492 (-) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  M9D40_RS06320 (M9D40_006320) coaE 1331503..1332123 (-) 621 WP_014391361.1 dephospho-CoA kinase -
  M9D40_RS06325 (M9D40_006325) - 1332138..1332860 (-) 723 WP_041423102.1 A24 family peptidase -
  M9D40_RS06330 (M9D40_006330) - 1332860..1334071 (-) 1212 WP_079157761.1 type II secretion system F family protein -
  M9D40_RS06335 (M9D40_006335) pilB 1334071..1335456 (-) 1386 WP_014391364.1 GspE/PulE family protein Machinery gene
  M9D40_RS06340 (M9D40_006340) pilA 1335456..1335890 (-) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  M9D40_RS06345 (M9D40_006345) ampD 1336050..1336616 (+) 567 WP_005719130.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9D40_RS06350 (M9D40_006350) rppH 1337202..1337798 (+) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  M9D40_RS06355 (M9D40_006355) - 1337801..1338595 (+) 795 WP_252759549.1 sulfite exporter TauE/SafE family protein -
  M9D40_RS06360 (M9D40_006360) lgt 1338605..1339417 (+) 813 WP_014391365.1 prolipoprotein diacylglyceryl transferase -
  M9D40_RS06365 (M9D40_006365) - 1339414..1340265 (+) 852 WP_014391366.1 thymidylate synthase -
  M9D40_RS06370 (M9D40_006370) tadA 1340280..1340780 (+) 501 WP_005755875.1 tRNA adenosine(34) deaminase TadA -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15058.48 Da        Isoelectric Point: 9.1281

>NTDB_id=782715 M9D40_RS06340 WP_005741096.1 1335456..1335890(-) (pilA) [Pasteurella multocida strain P2237]
MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR
ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide


Download         Length: 435 bp        

>NTDB_id=782715 M9D40_RS06340 WP_005741096.1 1335456..1335890(-) (pilA) [Pasteurella multocida strain P2237]
ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT
TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC
CTTATCGTGCCGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT
GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACGGTTAAAGCTGGGGTAATTACTGTTACTGGACAAGGGAG
CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC
AATCTGACATTTTCCCAGCAGGATTTTGTGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

63.194

100

0.632
  pilA Haemophilus influenzae Rd KW20

61.111

100

0.611
  pilA Glaesserella parasuis strain SC1401

52.349

100

0.542
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.806

100

0.368
  pilA Vibrio cholerae strain A1552

36.806

100

0.368
  pilA Vibrio cholerae C6706

36.806

100

0.368