Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   EcoliRb_RS23305 Genome accession   NZ_AP025682
Coordinates   4874395..4875915 (+) Length   506 a.a.
NCBI ID   WP_000058957.1    Uniprot ID   A0A0H3EP04
Organism   Escherichia coli strain Rb-50     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4869395..4880915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EcoliRb_RS23280 (TRECRb50_44530) ilvE 4870949..4871878 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  EcoliRb_RS23285 (TRECRb50_44540) ilvM 4871898..4872161 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  EcoliRb_RS23290 (TRECRb50_44550) ilvG 4872158..4873804 (-) 1647 WP_001012591.1 acetolactate synthase 2 catalytic subunit -
  EcoliRb_RS23295 ilvX 4873807..4873857 (-) 51 WP_001387183.1 peptide IlvX -
  EcoliRb_RS23300 ilvL 4873944..4874042 (-) 99 WP_001311244.1 ilv operon leader peptide -
  EcoliRb_RS23305 (TRECRb50_44560) comM 4874395..4875915 (+) 1521 WP_000058957.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  EcoliRb_RS23310 (TRECRb50_44570) maoP 4875940..4876278 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  EcoliRb_RS23315 (TRECRb50_44580) hdfR 4876397..4877236 (+) 840 WP_000379257.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55265.37 Da        Isoelectric Point: 7.4104

>NTDB_id=78252 EcoliRb_RS23305 WP_000058957.1 4874395..4875915(+) (comM) [Escherichia coli strain Rb-50]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=78252 EcoliRb_RS23305 WP_000058957.1 4874395..4875915(+) (comM) [Escherichia coli strain Rb-50]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCAGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTACTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGCGCGTTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCGCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCAATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAAAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EP04

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.317

100

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio cholerae strain A1552

58.765

99.209

0.583
  comM Vibrio cholerae O1 biovar El Tor strain E7946

58.765

99.209

0.583
  comM Vibrio campbellii strain DS40M4

57.769

99.209

0.573
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441

Multiple sequence alignment