Detailed information NTG0781687

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M0P28_RS03300
• Genome accession: NZ_CP116958
• Coordinates: 601125..601790 (+)
• Length: 221 a.a.
• NCBI ID: WP_013851600.1
• Uniprot ID: F5X5G1
• Organism: Streptococcus pasteurianus strain WUSP074
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 596125..606790

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M0P28_RS03270 (M0P28_03270)	-	596201..597379 (+)	1179	WP_048791494.1	MFS transporter	-
M0P28_RS03275 (M0P28_03275)	-	597434..598294 (+)	861	WP_048791495.1	helix-turn-helix transcriptional regulator	-
M0P28_RS03280 (M0P28_03280)	-	598353..598927 (+)	575	Protein_581	ROK family protein	-
M0P28_RS03285 (M0P28_03285)	-	599159..599440 (-)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
M0P28_RS03290 (M0P28_03290)	-	599415..600176 (-)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
M0P28_RS03295 (M0P28_03295)	-	600284..601030 (+)	747	WP_003063827.1	lysophospholipid acyltransferase family protein	-
M0P28_RS03300 (M0P28_03300)	comEA	601125..601790 (+)	666	WP_013851600.1	helix-hairpin-helix domain-containing protein	Machinery gene
M0P28_RS03305 (M0P28_03305)	comEC/celB	601783..604014 (+)	2232	WP_117479310.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M0P28_RS03310 (M0P28_03310)	holA	604088..605125 (+)	1038	WP_003063837.1	DNA polymerase III subunit delta	-
M0P28_RS03315 (M0P28_03315)	sodA	605214..605822 (+)	609	WP_013851603.1	superoxide dismutase SodA	-
M0P28_RS03320 (M0P28_03320)	-	605971..606696 (+)	726	WP_013851604.1	YebC/PmpR family DNA-binding transcriptional regulator	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23571.26 Da
• Isoelectric Point: 4.2822

>NTDB_id=781687 M0P28_RS03300 WP_013851600.1 601125..601790(+) (comEA) [Streptococcus pasteurianus strain WUSP074]
MIEEIKEKLLENKTLAAVLGIILVMIIGFFAWLNVTKTTADAQNDLPALSTTFSTSSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDESVIYVARVDENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=781687 M0P28_RS03300 WP_013851600.1 601125..601790(+) (comEA) [Streptococcus pasteurianus strain WUSP074]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGCAGCGGTATTGGGGATAATACTTGTCATGATAAT
CGGATTTTTTGCTTGGTTAAATGTGACAAAAACAACAGCGGATGCGCAAAATGACCTACCTGCCCTAAGCACGACTTTTT
CGACAAGTAGTAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAGACTCAAAAAGTGTTTGTAGATATC
AAAGGCGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAACGGTAGCCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATCTGGCTGAAAAAGTAACCGATGAATCGGTGATTTATGTGGCAAGAG
TTGATGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGATATTATTGATTA
CCGTGATGCTAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

3D structure

• AlphaFold DB: F5X5G1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.699	100	0.516
comEA/celA/cilE	Streptococcus mitis SK321	46.154	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.891	100	0.439
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae D39	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae R6	43.636	99.548	0.434
comEA	Latilactobacillus sakei subsp. sakei 23K	37.946	100	0.385