Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   MWM20_RS22925 Genome accession   NZ_AP025677
Coordinates   4743501..4745021 (+) Length   506 a.a.
NCBI ID   WP_000058956.1    Uniprot ID   -
Organism   Escherichia coli strain Rb-23     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4738501..4750021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM20_RS22900 (TRECRb23_43490) ilvE 4740055..4740984 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  MWM20_RS22905 (TRECRb23_43500) ilvM 4741004..4741267 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  MWM20_RS22910 (TRECRb23_43510) ilvG 4741264..4742910 (-) 1647 WP_001012583.1 acetolactate synthase 2 catalytic subunit -
  MWM20_RS22915 ilvX 4742913..4742963 (-) 51 WP_001387183.1 peptide IlvX -
  MWM20_RS22920 ilvL 4743050..4743148 (-) 99 WP_001311244.1 ilv operon leader peptide -
  MWM20_RS22925 (TRECRb23_43520) comM 4743501..4745021 (+) 1521 WP_000058956.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  MWM20_RS22930 (TRECRb23_43530) maoP 4745046..4745384 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  MWM20_RS22935 (TRECRb23_43540) hdfR 4745503..4746342 (+) 840 WP_000379257.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55235.35 Da        Isoelectric Point: 7.4104

>NTDB_id=78168 MWM20_RS22925 WP_000058956.1 4743501..4745021(+) (comM) [Escherichia coli strain Rb-23]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLA

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=78168 MWM20_RS22925 WP_000058956.1 4743501..4745021(+) (comM) [Escherichia coli strain Rb-23]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCAGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTGCCTATCGCTATTGCGTTACTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTTTGCGCATTTCTGGAAGGTAAGCACGCTCTCGAACACCCGAAACCAACTAATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCAATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAACAGCACCACCGTTAAACAACGTGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAATCCGGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACACTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGGCATA
A

Domains

Predicted by InterProScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.317

100

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio cholerae strain A1552

58.765

99.209

0.583
  comM Vibrio cholerae O1 biovar El Tor strain E7946

58.765

99.209

0.583
  comM Vibrio campbellii strain DS40M4

57.769

99.209

0.573
  comM Acinetobacter baylyi ADP1

48.996

98.419

0.482
  comM Legionella pneumophila str. Paris

47.791

98.419

0.47
  comM Legionella pneumophila strain ERS1305867

47.791

98.419

0.47
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.071

100

0.441

Multiple sequence alignment