Detailed information NTG0781627

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M0P24_RS07345
• Genome accession: NZ_CP116957
• Coordinates: 1470644..1471309 (-)
• Length: 221 a.a.
• NCBI ID: WP_013851600.1
• Uniprot ID: F5X5G1
• Organism: Streptococcus pasteurianus strain WUSP070
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1465644..1476309

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M0P24_RS07325 (M0P24_07325)	-	1465739..1466464 (-)	726	WP_013851604.1	YebC/PmpR family DNA-binding transcriptional regulator	-
M0P24_RS07330 (M0P24_07330)	sodA	1466613..1467221 (-)	609	WP_013851603.1	superoxide dismutase SodA	-
M0P24_RS07335 (M0P24_07335)	holA	1467310..1468347 (-)	1038	WP_043910377.1	DNA polymerase III subunit delta	-
M0P24_RS07340 (M0P24_07340)	comEC/celB	1468420..1470651 (-)	2232	WP_111718195.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M0P24_RS07345 (M0P24_07345)	comEA	1470644..1471309 (-)	666	WP_013851600.1	helix-hairpin-helix domain-containing protein	Machinery gene
M0P24_RS07350 (M0P24_07350)	-	1471404..1472150 (-)	747	WP_003063827.1	lysophospholipid acyltransferase family protein	-
M0P24_RS07355 (M0P24_07355)	-	1472258..1473019 (+)	762	WP_003063822.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
M0P24_RS07360 (M0P24_07360)	-	1472994..1473275 (+)	282	WP_003063819.1	GIY-YIG nuclease family protein	-
M0P24_RS07365 (M0P24_07365)	-	1473507..1474136 (-)	630	WP_013851599.1	ROK family protein	-
M0P24_RS07370 (M0P24_07370)	-	1474141..1475001 (-)	861	WP_003063816.1	helix-turn-helix transcriptional regulator	-
M0P24_RS07375 (M0P24_07375)	-	1475056..1476234 (-)	1179	WP_013851598.1	MFS transporter	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23571.26 Da
• Isoelectric Point: 4.2822

>NTDB_id=781627 M0P24_RS07345 WP_013851600.1 1470644..1471309(-) (comEA) [Streptococcus pasteurianus strain WUSP070]
MIEEIKEKLLENKTLAAVLGIILVMIIGFFAWLNVTKTTADAQNDLPALSTTFSTSSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGSRVTDVVKLAGGFTEDADKKSVNLAEKVTDESVIYVARVDENVAPATTNSQANGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=781627 M0P24_RS07345 WP_013851600.1 1470644..1471309(-) (comEA) [Streptococcus pasteurianus strain WUSP070]
ATGATTGAAGAAATAAAAGAAAAACTTTTGGAGAACAAAACGTTGGCAGCGGTATTGGGGATAATACTTGTCATGATAAT
CGGATTTTTTGCTTGGTTAAATGTGACAAAAACAACAGCGGATGCGCAAAATGACCTACCTGCCCTAAGCACGACTTTTT
CGACAAGTAGTAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAGACTCAAAAAGTGTTTGTAGATATT
AAAGGCGCTGTGAAAAATGAGGGGGTTTATGAGCTTTCAAACGGTAGCCGTGTGACAGATGTGGTCAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATCTGGCTGAAAAAGTAACCGATGAATCGGTGATTTATGTGGCAAGAG
TTGATGAAAATGTTGCTCCTGCAACGACTAATTCACAAGCAAATGGCAGCGCTCAACAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCGGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGATATTATTGACTA
CCGTGATGCTAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGTATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (157-219)

• SLBB domain (SLBB) (78-131)

3D structure

• AlphaFold DB: F5X5G1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.699	100	0.516
comEA/celA/cilE	Streptococcus mitis SK321	46.154	100	0.462
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus mitis NCTC 12261	43.891	100	0.439
comEA/celA/cilE	Streptococcus pneumoniae Rx1	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae D39	43.636	99.548	0.434
comEA/celA/cilE	Streptococcus pneumoniae R6	43.636	99.548	0.434
comEA	Latilactobacillus sakei subsp. sakei 23K	37.946	100	0.385