Detailed information NTG0779809

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: PMJ93_RS26870
• Genome accession: NZ_CP116721
• Coordinates: 5717143..5718843 (+)
• Length: 566 a.a.
• NCBI ID: WP_030048379.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 2867
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	5715125..5716153	5717143..5718843	flank	990

Gene organization within MGE regions

Location: 5715125..5718843

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PMJ93_RS26860 (PMJ93_26860)	-	5715125..5716153 (+)	1029	WP_050161940.1	IS30-like element ISPsp8 family transposase	-
PMJ93_RS26865 (PMJ93_26865)	-	5716448..5716912 (-)	465	WP_023129290.1	pilin	-
PMJ93_RS26870 (PMJ93_26870)	pilB	5717143..5718843 (+)	1701	WP_030048379.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62328.21 Da
• Isoelectric Point: 5.4470

>NTDB_id=779809 PMJ93_RS26870 WP_030048379.1 5717143..5718843(+) (pilB) [Pseudomonas aeruginosa strain 2867]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=779809 PMJ93_RS26870 WP_030048379.1 5717143..5718843(+) (pilB) [Pseudomonas aeruginosa strain 2867]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGCGCTATGGACGCTCTCGGCGACCTCGGTGACATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTATCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC
AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTATCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCACTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGTCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCTGAAGACCCTGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTCACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCGGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCTCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (190-457)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.79	100	0.569
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.521	98.763	0.509
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.345	94.17	0.493
pilB	Vibrio campbellii strain DS40M4	51.407	94.17	0.484
pilB/pilB1	Synechocystis sp. PCC 6803	37.763	100	0.412
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.909	97.173	0.378