NTDB

Overview

Name	pilB	Type	Machinery gene
Locus tag	OK431_RS26645	Genome accession	NZ_CP116682
Coordinates	5571772..5573472 (+)	Length	566 a.a.
NCBI ID	WP_010793793.1	Uniprot ID	A0A367MDN5
Organism	Pseudomonas aeruginosa strain HS337
Function	power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5566772..5578472

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OK431_RS26610 (OK431_26595)	fliP	5567489..5568241 (+)	753	WP_023435066.1	flagellar type III secretion system pore protein FliP	-
OK431_RS26615 (OK431_26600)	fliQ	5568286..5568555 (+)	270	WP_003083053.1	flagellar biosynthesis protein FliQ	-
OK431_RS26620 (OK431_26605)	fliR	5568555..5569331 (+)	777	WP_280168263.1	flagellar biosynthetic protein FliR	-
OK431_RS26625 (OK431_26610)	-	5569334..5569594 (+)	261	Protein_5265	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	-
OK431_RS26630 (OK431_26615)	-	5570591..5570833 (+)	243	WP_023082027.1	hypothetical protein	-
OK431_RS26640 (OK431_26625)	pilA	5571088..5571540 (-)	453	WP_114064701.1	pilin	Machinery gene
OK431_RS26645 (OK431_26630)	pilB	5571772..5573472 (+)	1701	WP_010793793.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OK431_RS26650 (OK431_26635)	pilC	5573476..5574696 (+)	1221	WP_009315898.1	type 4a pilus biogenesis protein PilC	Machinery gene
OK431_RS26655 (OK431_26640)	pilD	5574700..5575572 (+)	873	WP_003125204.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
OK431_RS26660 (OK431_26645)	coaE	5575569..5576180 (+)	612	WP_010793792.1	dephospho-CoA kinase	-
OK431_RS26665 (OK431_26650)	yacG	5576177..5576377 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
OK431_RS26670 (OK431_26655)	-	5576414..5576623 (-)	210	WP_003094660.1	hypothetical protein	-
OK431_RS26675 (OK431_26660)	-	5576729..5577418 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
OK431_RS26680 (OK431_26665)	-	5577415..5577885 (-)	471	WP_010793791.1	hypothetical protein	-
OK431_RS26685 (OK431_26670)	-	5577882..5578307 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-

Sequence

Protein

Download Length: 566 a.a. Molecular weight: 62354.24 Da Isoelectric Point: 5.8580

                                >NTDB_id=779090 OK431_RS26645 WP_010793793.1 5571772..5573472(+) (pilB) [Pseudomonas aeruginosa strain HS337]

MNNSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD

AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE

GLDIGSADKSTQEDASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL

KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG

MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL

ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE

DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV

NRVTKD

Nucleotide

Download Length: 1701 bp

                                >NTDB_id=779090 OK431_RS26645 WP_010793793.1 5571772..5573472(+) (pilB) [Pseudomonas aeruginosa strain HS337]

ATGAACAACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA

GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACACACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC

TGGCCGAATTGTCCGCCGAGCAGTTCGGCATCGCTTATTGCGACCTGAACAGCCTGGACAAGGAAAGCTTTCCGCGGGAC

GCTATCAGCGAGAAGCTGGTCCGCCAACACCGGGTCATCCCTCTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC

CGACCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG

ACAAGCTTGGCCTGGCCATCGACAAGCTGTTCGAAAGCGCTACCGACGGTCTCGCAGGCCTCGACGATGTCGACCTCGAG

GGACTGGATATCGGTAGCGCGGACAAGTCCACCCAAGAGGATGCCAGCGCAGAAGCGGACGACGCACCTGTAGTACGTTT

CGTCAACAAGATGCTGCTGGATGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG

TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGTATCTCTGCTCGTCTC

AAGGTAATGGCCGGCCTGGATATCTCCGAACGACGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGACCAA

GTCCATCGACTTTCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG

CACAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCGCAGGGC

ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTGAATACCACCGA

CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG

GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG

GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA

GACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC

TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCGGAA

GACAAGATCGGCACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT

TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG

CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC

AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A367MDN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilB	Acinetobacter baumannii D1279779	57.517	100	0.581
pilB	Acinetobacter baylyi ADP1	57.194	99.47	0.569
pilB	Legionella pneumophila strain ERS1305867	55.124	100	0.551
pilB	Vibrio parahaemolyticus RIMD 2210633	50.887	99.647	0.507
pilF	Neisseria gonorrhoeae MS11	50.534	99.293	0.502
pilB	Vibrio cholerae strain A1552	50	99.647	0.498
pilB	Vibrio campbellii strain DS40M4	49.645	99.647	0.495
pilF	Thermus thermophilus HB27	39.572	99.117	0.392
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.417	93.11	0.376
pilB/pilB1	Synechocystis sp. PCC 6803	38.764	94.346	0.366

Detailed information NTG0779090