Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   PHA78_RS00825 Genome accession   NZ_CP116604
Coordinates   140255..140689 (+) Length   144 a.a.
NCBI ID   WP_172115686.1    Uniprot ID   -
Organism   Streptococcus sp. HN38     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 135255..145689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PHA78_RS00790 - 135893..136252 (+) 360 WP_024377796.1 DUF1033 family protein -
  PHA78_RS00795 - 136397..137347 (-) 951 WP_272158320.1 S66 peptidase family protein -
  PHA78_RS00800 comYA 137433..138383 (+) 951 WP_043026808.1 competence type IV pilus ATPase ComGA Machinery gene
  PHA78_RS00805 comYB 138295..139332 (+) 1038 WP_272158556.1 competence type IV pilus assembly protein ComGB Machinery gene
  PHA78_RS00810 comYC 139334..139615 (+) 282 WP_272158321.1 competence type IV pilus major pilin ComGC Machinery gene
  PHA78_RS00815 comGD 139596..140003 (+) 408 WP_272158322.1 competence type IV pilus minor pilin ComGD -
  PHA78_RS00820 comYE 139975..140268 (+) 294 WP_272158323.1 competence type IV pilus minor pilin ComGE Machinery gene
  PHA78_RS00825 comGF/cglF 140255..140689 (+) 435 WP_172115686.1 competence type IV pilus minor pilin ComGF Machinery gene
  PHA78_RS00830 comGG 140667..141194 (+) 528 WP_272158324.1 competence type IV pilus minor pilin ComGG -
  PHA78_RS00835 comYH 141251..142204 (+) 954 WP_272158325.1 class I SAM-dependent methyltransferase Machinery gene
  PHA78_RS00840 - 142254..143441 (+) 1188 WP_105202630.1 acetate kinase -
  PHA78_RS00845 - 143758..144309 (+) 552 WP_202105774.1 folate family ECF transporter S component -
  PHA78_RS00850 - 144363..145625 (+) 1263 WP_272158326.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16423.73 Da        Isoelectric Point: 6.9827

>NTDB_id=778747 PHA78_RS00825 WP_172115686.1 140255..140689(+) (comGF/cglF) [Streptococcus sp. HN38]
MLKSKVLAFTLLECLVALVIVSGSLLIFEGLSKLLVQEAHYQGQTVQKEWLVFSSQLRSEWDQSELVKVENGKVYVNKDG
QALAFGKSRSDDFRKTNDRGQGYQPMIYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=778747 PHA78_RS00825 WP_172115686.1 140255..140689(+) (comGF/cglF) [Streptococcus sp. HN38]
TTGTTAAAAAGTAAGGTTCTTGCTTTTACCCTATTGGAATGCTTGGTGGCTCTAGTTATCGTTTCAGGCAGCCTCTTGAT
TTTTGAAGGCTTGTCAAAGTTGTTGGTTCAAGAAGCACATTACCAAGGTCAGACCGTTCAAAAGGAGTGGCTAGTATTTT
CTAGTCAGTTGCGGTCGGAATGGGACCAGTCGGAATTGGTCAAGGTTGAAAATGGCAAGGTCTATGTCAATAAGGATGGA
CAAGCATTAGCCTTTGGCAAGTCACGTTCGGATGATTTTCGGAAAACAAATGACAGAGGGCAGGGCTATCAGCCCATGAT
TTATCAAGTAGACAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(37-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae TIGR4

56.028

97.917

0.549
  comGF/cglF Streptococcus pneumoniae Rx1

56.028

97.917

0.549
  comGF/cglF Streptococcus pneumoniae D39

56.028

97.917

0.549
  comGF/cglF Streptococcus pneumoniae R6

56.028

97.917

0.549
  comGF/cglF Streptococcus mitis NCTC 12261

55.224

93.056

0.514
  comGF/cglF Streptococcus mitis SK321

53.731

93.056

0.5
  comYF Streptococcus mutans UA140

48.611

100

0.486
  comYF Streptococcus mutans UA159

47.917

100

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

44.681

97.917

0.438