Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PL263_RS15920 Genome accession   NZ_CP116398
Coordinates   3548407..3549561 (+) Length   384 a.a.
NCBI ID   WP_278210285.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3543407..3554561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL263_RS15900 (PL263_15895) - 3543566..3543790 (-) 225 WP_278210280.1 sulfurtransferase TusA family protein -
  PL263_RS15905 (PL263_15900) - 3543787..3544830 (-) 1044 WP_140913450.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  PL263_RS15910 (PL263_15905) - 3544998..3545630 (-) 633 WP_278210281.1 response regulator transcription factor -
  PL263_RS15915 (PL263_15910) - 3545627..3548248 (-) 2622 WP_278210283.1 PAS domain-containing protein -
  PL263_RS15920 (PL263_15915) pilU 3548407..3549561 (+) 1155 WP_278210285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PL263_RS15925 (PL263_15920) - 3549582..3549905 (-) 324 WP_278210286.1 NGG1p interacting factor NIF3 -
  PL263_RS15930 (PL263_15925) - 3549951..3550100 (-) 150 WP_186289641.1 hypothetical protein -
  PL263_RS15935 (PL263_15930) lexA 3550113..3550742 (-) 630 WP_278210288.1 transcriptional repressor LexA -
  PL263_RS15940 (PL263_15935) - 3550799..3551026 (-) 228 WP_278210289.1 hypothetical protein -
  PL263_RS15945 (PL263_15940) ftsH 3551324..3553231 (+) 1908 WP_278210291.1 ATP-dependent zinc metalloprotease FtsH -
  PL263_RS15950 (PL263_15945) - 3553306..3554028 (-) 723 WP_140913458.1 cytochrome c1 -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42449.59 Da        Isoelectric Point: 6.3220

>NTDB_id=777141 PL263_RS15920 WP_278210285.1 3548407..3549561(+) (pilU) [Methylomonas sp. EFPC3]
MEFRDYLKILVQHDGSDLYLTAGAPPAAKFQGTLKPLENVKLTPERIKQIADSVMDHEQRASFEHVPEMNLAITEEGIGR
FRVNIFKQRNCYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSNSSGHIITIED
PIEFVHPHKRSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNLQAFVSQRLVPTIEGKRVAAIEILLGTKLVSDLIHKGDIHAIKEAMEKSENIGMQTFDSHL
LKLYKSGVISLEEALRNSDSPNNLKLKINLSEGLGSATKQTGALGSLALEEISKKEGDDGDGGH

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=777141 PL263_RS15920 WP_278210285.1 3548407..3549561(+) (pilU) [Methylomonas sp. EFPC3]
ATGGAATTTAGAGACTATTTGAAAATCCTGGTGCAGCACGACGGTTCCGATTTGTATTTGACGGCCGGCGCGCCGCCGGC
CGCCAAATTCCAAGGCACCTTGAAGCCGTTGGAAAACGTCAAGCTTACCCCGGAGCGGATCAAGCAAATCGCCGACAGCG
TGATGGACCACGAGCAACGCGCCAGTTTCGAGCACGTGCCGGAGATGAATCTGGCCATCACTGAAGAGGGCATCGGCCGG
TTCCGGGTCAATATTTTCAAGCAGCGCAATTGTTATGCCTTGGTAATCCGCAACATCAAGGTGGATATTCCGAATGCCGA
CGTTTTGGGTCTGCCGCAGATTTTGAAAGACAAAATCATGGAGAAGCGCGGCCTGATTTTGTTCGTCGGCGGCACCGGCT
CCGGTAAATCGACTTCGCTGGCGGCCTTGATCGATTACCGCAACAGCAATTCGTCGGGCCACATCATCACCATCGAAGAT
CCGATCGAATTCGTGCATCCGCATAAACGCTCGTTGGTCAATCAGCGCGAAGTCGGGGTCGATACCCTAAGCTACGAAGA
CGCCTTGAAGAATACGCTGCGCCAAGCGCCCGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACCATGGAGCATG
CGTTGGCCTTTGCCGAAACCGGCCATTTATGCCTGTCGACCTTGCATGCCAATAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTTCCGGAAGAGCGCCGGCCGCAATTGTTGATGGATTTGTCTTTGAATTTGCAGGCCTTTGTCTCGCAACG
CCTGGTGCCGACCATAGAAGGCAAGCGGGTGGCGGCGATCGAAATTCTGTTAGGTACCAAACTGGTCAGTGATTTGATCC
ACAAGGGCGACATTCATGCCATCAAGGAAGCGATGGAAAAGTCGGAAAATATCGGTATGCAGACCTTCGACAGCCATCTG
TTGAAGCTGTACAAAAGCGGCGTGATTTCGTTGGAAGAAGCGCTGCGCAATTCGGATTCGCCGAACAATCTGAAACTGAA
AATCAATTTGAGCGAGGGTTTGGGTTCGGCTACCAAACAAACCGGAGCGCTGGGCAGTCTGGCTTTGGAAGAGATCAGCA
AGAAGGAAGGCGACGACGGCGACGGAGGGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.12

91.406

0.531
  pilU Acinetobacter baylyi ADP1

55.367

92.188

0.51
  pilU Vibrio cholerae strain A1552

49.036

94.531

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.052

100

0.404
  pilT Legionella pneumophila strain Lp02

42.424

85.938

0.365
  pilT Legionella pneumophila strain ERS1305867

42.424

85.938

0.365