Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PK654_RS12940 Genome accession   NZ_CP116383
Coordinates   2770799..2771212 (+) Length   137 a.a.
NCBI ID   WP_271696184.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43137     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2765799..2776212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PK654_RS12925 (PK654_12925) pdhR 2767948..2768715 (-) 768 WP_271696182.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PK654_RS12930 (PK654_12930) ampD 2768989..2769534 (-) 546 WP_271696183.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PK654_RS12935 (PK654_12935) nadC 2769705..2770592 (+) 888 WP_271698881.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PK654_RS12940 (PK654_12940) pilA 2770799..2771212 (+) 414 WP_271696184.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  PK654_RS12945 (PK654_12945) pilB 2771218..2772429 (+) 1212 WP_271696185.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  PK654_RS12950 (PK654_12950) - 2772430..2773653 (+) 1224 WP_271696186.1 type II secretion system F family protein -
  PK654_RS12955 (PK654_12955) pilD 2773687..2774556 (+) 870 WP_271696187.1 A24 family peptidase Machinery gene
  PK654_RS12960 (PK654_12960) coaE 2774558..2775169 (+) 612 WP_271696188.1 dephospho-CoA kinase -
  PK654_RS12965 (PK654_12965) zapD 2775195..2775935 (+) 741 WP_271696189.1 cell division protein ZapD -
  PK654_RS12970 (PK654_12970) yacG 2775993..2776184 (+) 192 WP_271696190.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 15087.53 Da        Isoelectric Point: 10.2275

>NTDB_id=776863 PK654_RS12940 WP_271696184.1 2770799..2771212(+) (pilA) [Vibrio sp. SCSIO 43137]
MCNKKRQGFTLIELMIVITIIGLLSTFAIPAYQSYLKKGEITSAMATLRALITPAEILYLEHGNLSGKNTLNDLGSSANA
NPLGTIAISKGNSLTFTFDKSNQFSKQSKLTYQRTATGWQCTRHKIETKLKGCPEQK

Nucleotide


Download         Length: 414 bp        

>NTDB_id=776863 PK654_RS12940 WP_271696184.1 2770799..2771212(+) (pilA) [Vibrio sp. SCSIO 43137]
ATGTGTAACAAGAAGCGGCAAGGATTCACCCTTATTGAACTGATGATAGTTATTACTATTATCGGTTTACTATCTACCTT
TGCTATACCTGCCTATCAGAGCTACCTGAAAAAAGGCGAAATAACCTCTGCCATGGCAACCCTCAGGGCTCTGATTACGC
CTGCAGAGATATTGTATCTGGAACACGGCAACCTATCAGGAAAGAACACACTGAATGATCTTGGCAGCTCCGCCAACGCC
AATCCACTCGGCACCATCGCTATATCAAAGGGAAACAGCCTTACCTTCACCTTCGATAAAAGCAATCAGTTTTCCAAACA
GAGCAAACTCACTTATCAGCGAACAGCAACAGGCTGGCAATGCACCCGCCACAAAATAGAGACTAAGCTAAAAGGCTGTC
CTGAACAGAAGTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(39-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

49.254

97.81

0.482
  pilA Pseudomonas aeruginosa PAK

39.456

100

0.423
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.694

100

0.372
  pilA Vibrio cholerae strain A1552

34.694

100

0.372
  pilA Vibrio cholerae C6706

34.694

100

0.372