Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG907_RS03045 Genome accession   NZ_CP115955
Coordinates   649305..649826 (+) Length   173 a.a.
NCBI ID   WP_193026452.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2340     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644305..654826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG907_RS03020 recX 644682..645161 (+) 480 WP_193026457.1 recombination regulator RecX -
  PG907_RS03025 - 645437..646063 (+) 627 WP_193026456.1 DUF2889 domain-containing protein -
  PG907_RS03030 sucC 646130..647296 (+) 1167 WP_193026455.1 ADP-forming succinate--CoA ligase subunit beta -
  PG907_RS03035 sucD 647382..648263 (+) 882 WP_193026454.1 succinate--CoA ligase subunit alpha -
  PG907_RS03040 - 648413..649111 (+) 699 WP_193026453.1 TerC family protein -
  PG907_RS03045 pilA 649305..649826 (+) 522 WP_193026452.1 pilin Machinery gene
  PG907_RS03050 pilA 650099..650605 (+) 507 WP_193026451.1 pilin Machinery gene
  PG907_RS03055 - 650679..652412 (+) 1734 WP_193028552.1 PglL family O-oligosaccharyltransferase -
  PG907_RS03060 moaC 652484..652960 (-) 477 WP_193026450.1 cyclic pyranopterin monophosphate synthase MoaC -
  PG907_RS03065 - 653054..654742 (+) 1689 WP_193026449.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17888.55 Da        Isoelectric Point: 9.8138

>NTDB_id=774598 PG907_RS03045 WP_193026452.1 649305..649826(+) (pilA) [Ralstonia pseudosolanacearum strain RUN2340]
MASKHSRNRWRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVVEGLSLAAQAKALVVENAANAQSSLSLGSASL
PASRNVSALNIDSSTGEIAVVYASAVTGAEANTLVLTPYSGSSSSLANLQAGSAPPTMVLWVCTAAGKSMPVNTVTQRTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=774598 PG907_RS03045 WP_193026452.1 649305..649826(+) (pilA) [Ralstonia pseudosolanacearum strain RUN2340]
ATGGCATCGAAGCACAGCAGGAACAGGTGGCGCGGCCAGCGCGGCTTTACATTGATTGAATTGATGATCGTGGTGGCGAT
CATCGGGATATTGGCGGCGATTGCGATTCCGGCCTATCAGGACTACACGATCCGCGCGCGCGTGGTTGAGGGGCTGTCGC
TGGCAGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCTGCCAACGCGCAGAGTAGCCTGTCACTCGGCTCTGCATCGTTG
CCGGCCAGCAGGAACGTCAGTGCGCTGAATATTGATTCGTCCACGGGCGAAATCGCGGTGGTCTATGCATCTGCTGTTAC
GGGAGCGGAGGCGAATACGTTGGTATTGACCCCGTACTCCGGAAGCTCGTCATCACTTGCCAATCTGCAGGCGGGTTCAG
CGCCGCCGACAATGGTGCTGTGGGTGTGTACAGCCGCCGGCAAGTCGATGCCAGTCAATACTGTCACCCAGAGAACGACG
GCTACGCTGCCACCGAAGTACGCGCCCGCAGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

53.977

100

0.549
  pilA2 Legionella pneumophila str. Paris

46.061

95.376

0.439
  pilA2 Legionella pneumophila strain ERS1305867

45.455

95.376

0.434
  comP Acinetobacter baylyi ADP1

43.478

93.064

0.405
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.602

100

0.393