Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG903_RS02630 Genome accession   NZ_CP115952
Coordinates   557522..558043 (+) Length   173 a.a.
NCBI ID   WP_043908616.1    Uniprot ID   -
Organism   Ralstonia solanacearum CFBP2957     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552522..563043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG903_RS02605 recX 552908..553387 (+) 480 WP_003269433.1 recombination regulator RecX -
  PG903_RS02610 - 553661..554287 (+) 627 WP_013206907.1 DUF2889 domain-containing protein -
  PG903_RS02615 sucC 554356..555522 (+) 1167 WP_003261636.1 ADP-forming succinate--CoA ligase subunit beta -
  PG903_RS02620 sucD 555601..556482 (+) 882 WP_003269430.1 succinate--CoA ligase subunit alpha -
  PG903_RS02625 - 556640..557338 (+) 699 WP_013206906.1 TerC family protein -
  PG903_RS02630 pilA 557522..558043 (+) 522 WP_043908616.1 pilin Machinery gene
  PG903_RS02635 pilA 558311..558817 (+) 507 WP_013206904.1 pilin Machinery gene
  PG903_RS02640 - 558892..560625 (+) 1734 WP_013206903.1 PglL family O-oligosaccharyltransferase -
  PG903_RS02645 moaC 560702..561178 (-) 477 WP_013206902.1 cyclic pyranopterin monophosphate synthase MoaC -
  PG903_RS02650 - 561280..562992 (+) 1713 WP_043908614.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17663.44 Da        Isoelectric Point: 10.2418

>NTDB_id=774576 PG903_RS02630 WP_043908616.1 557522..558043(+) (pilA) [Ralstonia solanacearum CFBP2957]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAALAIPAYQDYTIRARVVEGLSLAAQAKALVVENAANAQSSLALGSASL
PASKNVSALNIDASTGEIAVVYATAVTAGGANTLVLTPYSGSSSALANLRAGSAPPAMVLWVCAAAGKSMPVTTVTQNTT
PTLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=774576 PG903_RS02630 WP_043908616.1 557522..558043(+) (pilA) [Ralstonia solanacearum CFBP2957]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGTCAGCGCGGCTTTACGTTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATACTGGCGGCGCTGGCGATTCCGGCGTACCAGGACTACACGATCCGGGCGCGGGTGGTTGAGGGGCTGTCGC
TGGCCGCCCAGGCCAAGGCACTGGTGGTTGAGAATGCTGCCAACGCCCAGAGCAGCTTGGCGCTCGGCTCCGCATCATTG
CCGGCGAGCAAGAATGTCAGTGCGCTGAATATCGATGCGTCTACCGGCGAGATTGCGGTGGTCTATGCGACAGCCGTCAC
GGCCGGTGGGGCGAACACGCTGGTCTTGACCCCATACTCTGGAAGTTCGTCAGCGCTTGCCAACTTGCGGGCTGGCTCTG
CACCGCCTGCAATGGTGTTGTGGGTGTGCGCTGCAGCCGGAAAGTCAATGCCGGTCACCACCGTCACCCAGAACACGACG
CCTACGTTGCCGCCGAAATATGCGCCTGCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)

Pilin (bacterial filament) (Pilin)

(45-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

53.977

100

0.549
  pilA2 Legionella pneumophila str. Paris

46.061

95.376

0.439
  pilA2 Legionella pneumophila strain ERS1305867

44.848

95.376

0.428
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.41
  comP Acinetobacter baylyi ADP1

42.236

93.064

0.393