Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG906_RS03165 Genome accession   NZ_CP115944
Coordinates   660791..661303 (+) Length   170 a.a.
NCBI ID   WP_197330892.1    Uniprot ID   G3A4T7
Organism   Ralstonia syzygii R24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655791..666303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG906_RS03145 sucD 656220..657101 (+) 882 WP_197332581.1 succinate--CoA ligase subunit alpha -
  PG906_RS03150 ltrA 657557..658924 (+) 1368 WP_197332989.1 group II intron reverse transcriptase/maturase -
  PG906_RS03155 - 659113..659811 (+) 699 WP_197332582.1 TerC family protein -
  PG906_RS03160 pilA 660002..660523 (+) 522 WP_197332583.1 pilin Machinery gene
  PG906_RS03165 pilA 660791..661303 (+) 513 WP_197330892.1 pilin Machinery gene
  PG906_RS03170 - 661388..663118 (+) 1731 WP_197332584.1 PglL family O-oligosaccharyltransferase -
  PG906_RS03175 - 663385..664291 (-) 907 Protein_632 IS481 family transposase -
  PG906_RS03180 - 664387..665424 (-) 1038 WP_197332585.1 ISL3 family transposase -
  PG906_RS03185 moaC 665555..666034 (-) 480 WP_197332586.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 170 a.a.        Molecular weight: 17153.89 Da        Isoelectric Point: 10.0502

>NTDB_id=774542 PG906_RS03165 WP_197330892.1 660791..661303(+) (pilA) [Ralstonia syzygii R24]
MKSMRRLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTIRARVTEGLSLAAQAKTLVAENAANAASDLSAGSSSFV
ATKNVATLAIAAATGDITIGYSTPVGTGTPTLVLTPGAGGSALAINSAPGAPIVWTCYSLGKTAPSSAGGVKYNVTAPTL
APKYAPAECR

Nucleotide


Download         Length: 513 bp        

>NTDB_id=774542 PG906_RS03165 WP_197330892.1 660791..661303(+) (pilA) [Ralstonia syzygii R24]
ATGAAGTCGATGCGTCGTCTGAACAAGCGTGTCCAGAAGGGCTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCCGCGATTGCTATTCCGGCCTATCAGGACTACACCATCCGCGCACGCGTGACCGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGACGCTGGTGGCTGAGAACGCAGCGAATGCAGCGAGCGATCTGTCGGCGGGTTCGTCGTCGTTCGTT
GCCACCAAGAACGTTGCAACGCTTGCGATTGCTGCGGCTACTGGTGACATCACCATTGGTTATTCGACGCCGGTTGGGAC
CGGTACGCCGACGCTGGTGCTGACGCCTGGTGCCGGTGGTTCGGCCCTGGCAATTAACAGTGCTCCTGGCGCCCCGATCG
TGTGGACCTGCTATTCGTTGGGCAAGACTGCACCTAGCAGCGCAGGTGGCGTGAAATATAACGTGACCGCCCCTACGTTG
GCCCCCAAGTATGCACCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)

Pilin (bacterial filament) (Pilin)

(44-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3A4T7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

66.667

100

0.694
  pilA2 Legionella pneumophila str. Paris

50.92

95.882

0.488
  pilA2 Legionella pneumophila strain ERS1305867

49.693

95.882

0.476
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.458

100

0.412
  comP Acinetobacter baylyi ADP1

43.125

94.118

0.406