Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG906_RS03160 Genome accession   NZ_CP115944
Coordinates   660002..660523 (+) Length   173 a.a.
NCBI ID   WP_197332583.1    Uniprot ID   G3A5Q2
Organism   Ralstonia syzygii R24     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655002..665523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG906_RS03145 sucD 656220..657101 (+) 882 WP_197332581.1 succinate--CoA ligase subunit alpha -
  PG906_RS03150 ltrA 657557..658924 (+) 1368 WP_197332989.1 group II intron reverse transcriptase/maturase -
  PG906_RS03155 - 659113..659811 (+) 699 WP_197332582.1 TerC family protein -
  PG906_RS03160 pilA 660002..660523 (+) 522 WP_197332583.1 pilin Machinery gene
  PG906_RS03165 pilA 660791..661303 (+) 513 WP_197330892.1 pilin Machinery gene
  PG906_RS03170 - 661388..663118 (+) 1731 WP_197332584.1 PglL family O-oligosaccharyltransferase -
  PG906_RS03175 - 663385..664291 (-) 907 Protein_632 IS481 family transposase -
  PG906_RS03180 - 664387..665424 (-) 1038 WP_197332585.1 ISL3 family transposase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17671.28 Da        Isoelectric Point: 9.5797

>NTDB_id=774541 PG906_RS03160 WP_197332583.1 660002..660523(+) (pilA) [Ralstonia syzygii R24]
MASKHSGSRLRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRSRVVEGLSLAAQAKALVVENAANAQSSLAQGASTL
QVDRNVSALNINSTTGEIAVVFTSAVAGTGANTLVLTPYSGNSSSVANLQAGSAPPGMVLWVCAAAGKAMPVNTVTQQTT
ATLPPKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=774541 PG906_RS03160 WP_197332583.1 660002..660523(+) (pilA) [Ralstonia syzygii R24]
ATGGCATCGAAGCACAGTGGGAGCAGGCTGCGCGGCCAGCGCGGCTTTACGTTGATCGAGTTGATGATCGTGGTCGCGAT
CATCGGGATCTTGGCGGCGATTGCGATTCCGGCGTATCAGGACTACACCATTCGGTCGCGGGTGGTTGAGGGGCTGTCGC
TGGCCGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCAGCCAACGCGCAGAGCAGTCTGGCGCAAGGCGCCTCGACGTTG
CAGGTCGACAGGAACGTCAGCGCTTTGAATATCAACTCGACTACGGGCGAGATAGCCGTGGTCTTCACGTCTGCTGTTGC
GGGTACGGGAGCGAATACGCTGGTATTGACCCCGTACTCCGGAAACTCTTCCTCGGTTGCAAACTTGCAGGCGGGTTCGG
CGCCGCCAGGAATGGTGCTGTGGGTATGTGCTGCCGCCGGCAAGGCAATGCCAGTCAATACTGTCACCCAGCAAACGACG
GCTACGTTGCCACCGAAATACGCGCCCTCGGAGTGTCGTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(11-35)

Pilin (bacterial filament) (Pilin)

(45-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3A5Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

53.757

100

0.538
  pilA2 Legionella pneumophila str. Paris

45.455

95.376

0.434
  pilA2 Legionella pneumophila strain ERS1305867

44.848

95.376

0.428
  comP Acinetobacter baylyi ADP1

40.223

100

0.416
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.41