Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PG915_RS02815 Genome accession   NZ_CP115920
Coordinates   608906..610594 (-) Length   562 a.a.
NCBI ID   WP_353498649.1    Uniprot ID   A0AAU8BMD6
Organism   Vibrio chaetopteri strain CB1-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603906..615594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG915_RS02785 (PG915_02780) rplS 604283..604636 (+) 354 WP_006074418.1 50S ribosomal protein L19 -
  PG915_RS02790 (PG915_02785) yacG 605120..605314 (-) 195 WP_353497776.1 DNA gyrase inhibitor YacG -
  PG915_RS02795 (PG915_02790) zapD 605368..606108 (-) 741 WP_338163453.1 cell division protein ZapD -
  PG915_RS02800 (PG915_02795) coaE 606140..606748 (-) 609 WP_353497777.1 dephospho-CoA kinase -
  PG915_RS02805 (PG915_02800) pilD 606748..607620 (-) 873 WP_353497778.1 prepilin peptidase Machinery gene
  PG915_RS02810 (PG915_02805) pilC 607663..608889 (-) 1227 WP_353497779.1 type II secretion system F family protein Machinery gene
  PG915_RS02815 (PG915_02810) pilB 608906..610594 (-) 1689 WP_353498649.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PG915_RS02820 (PG915_02815) - 610601..611026 (-) 426 WP_353497780.1 pilin -
  PG915_RS02825 (PG915_02820) nadC 611296..612186 (-) 891 WP_353497781.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PG915_RS02830 (PG915_02825) ampD 612372..612935 (+) 564 WP_353498650.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PG915_RS02835 (PG915_02830) pdhR 613341..614108 (+) 768 WP_112459454.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62631.67 Da        Isoelectric Point: 5.3716

>NTDB_id=774445 PG915_RS02815 WP_353498649.1 608906..610594(-) (pilB) [Vibrio chaetopteri strain CB1-14]
MNSRLPIVLNDAHLISTTQKEQLVEWASLNAGSIPERIVDLGWISAQELNSFLGKLFSLTIKELHDYNPSDLCQKIGLRD
TIIKHRALPLSLENHCLTIAISDPTVVETENDFRFATGLQTELVLANCQDIRSTIRKLFGDNNLDSSSETKQLTSDDLAN
LVQVSDAEIDNIEDLSQDDSPVSRFIHQVLMDAVRKKASDIHFEPYEHLYRIRLRCDGILIETHQPATQLSRRLAARIKI
LAKLDIAERRLPQDGRLKLRLSETASIDMRVSTLPTMWGEKIVLRLLDGDSANLDIDQLGYNELQKSLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLNILNTAERNVSTAEDPVEIHLDGINQVQINPKIGFDFSTALRSFLRQDPDVVMLGEIRDIET
AEIAVKAAQTGHLVLSTLHTNSAAETVMRLKNMGVESINLASSLSLIIAQRLARKLCQHCKVIDPMLPPLRHSLAISDDE
LIYQASEQGCNHCNYGYLGRTGIYEVMECTHEIANALLIGASMQEIEALAQSQGMTTLKQSGIEKLRDGVTSYQELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=774445 PG915_RS02815 WP_353498649.1 608906..610594(-) (pilB) [Vibrio chaetopteri strain CB1-14]
ATAAATTCTCGCCTTCCAATTGTACTTAATGATGCCCATCTAATATCCACTACTCAAAAAGAGCAATTGGTTGAGTGGGC
TTCATTAAATGCAGGTTCAATACCGGAGAGGATTGTAGACTTGGGCTGGATCTCAGCCCAAGAGTTAAATAGCTTCCTAG
GAAAGCTTTTCTCTCTCACCATTAAAGAACTGCATGACTACAACCCTTCTGATTTGTGCCAAAAGATAGGCCTTCGCGAT
ACAATCATTAAACATCGTGCTCTTCCCTTGAGCCTCGAAAATCACTGTTTAACTATCGCAATATCCGATCCTACTGTAGT
AGAAACTGAAAATGACTTTCGCTTTGCTACGGGGCTGCAAACTGAGTTGGTGCTAGCTAATTGCCAAGACATTCGCTCTA
CAATACGTAAACTTTTCGGTGACAATAACCTAGACTCCAGCTCAGAAACCAAACAACTCACCTCAGACGATCTCGCCAAC
CTAGTCCAAGTCAGCGATGCCGAAATCGACAACATCGAAGATCTCTCCCAAGATGATTCGCCAGTCAGTCGCTTTATCCA
CCAAGTCCTCATGGATGCGGTACGCAAAAAAGCTTCTGATATCCATTTCGAGCCCTATGAGCATCTATACCGCATTCGCT
TACGCTGCGATGGGATCTTGATTGAGACCCACCAGCCTGCCACTCAGTTGAGTCGACGTTTGGCGGCGAGGATCAAAATC
CTAGCAAAGCTAGATATCGCCGAGCGCCGATTGCCGCAGGATGGGCGTTTGAAATTGAGGCTATCGGAAACTGCTTCGAT
TGATATGCGCGTATCGACCTTACCTACTATGTGGGGTGAGAAGATCGTATTACGTCTGCTGGATGGCGATTCTGCTAATC
TCGATATCGATCAACTGGGTTATAACGAACTGCAAAAATCCCTCTACCTTGCTGCACTTAAAAAGCCACAAGGTATGATC
TTAATGACAGGGCCGACGGGAAGCGGAAAAACCGTTTCTTTGTATACTGGACTCAATATCCTCAATACCGCTGAGCGCAA
CGTATCAACGGCGGAAGATCCGGTTGAGATCCACCTAGATGGGATCAATCAGGTGCAAATCAATCCAAAGATCGGCTTTG
ATTTTTCGACCGCGCTGCGATCGTTTCTACGTCAAGATCCGGATGTGGTGATGCTGGGAGAGATCCGTGATATTGAAACG
GCGGAGATCGCGGTGAAAGCAGCGCAAACCGGCCACTTAGTGCTTTCTACTCTGCACACCAACTCAGCGGCAGAGACGGT
GATGCGACTTAAAAATATGGGCGTTGAAAGCATTAACCTCGCCTCTTCACTCTCACTCATCATTGCTCAGCGCCTAGCCC
GCAAGCTGTGTCAGCATTGTAAGGTTATCGACCCTATGTTACCGCCACTGCGTCACTCCCTCGCCATTAGTGACGATGAA
CTTATCTATCAAGCCAGCGAGCAGGGCTGCAATCATTGTAACTATGGCTATTTGGGGCGTACAGGCATTTATGAAGTGAT
GGAGTGTACCCACGAAATCGCCAATGCTTTGCTTATCGGTGCAAGCATGCAAGAGATAGAAGCATTGGCACAATCTCAAG
GGATGACCACTCTCAAACAATCGGGCATTGAAAAGCTTCGAGATGGCGTGACCAGCTATCAAGAGTTGCAGCGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

64.413

100

0.644
  pilB Vibrio parahaemolyticus RIMD 2210633

62.811

100

0.628
  pilB Vibrio campbellii strain DS40M4

62.234

100

0.625
  pilB Legionella pneumophila strain ERS1305867

46.416

99.288

0.461
  pilB Acinetobacter baylyi ADP1

46.461

98.043

0.456
  pilB Acinetobacter baumannii D1279779

48.924

90.925

0.445
  pilF Neisseria gonorrhoeae MS11

42.473

99.288

0.422