Detailed information NTG0773403

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: O6P39_RS01895
• Genome accession: NZ_CP115669
• Coordinates: 402960..404105 (+)
• Length: 381 a.a.
• NCBI ID: WP_275609795.1
• Uniprot ID: -
• Organism: Pseudomonas sp. PSE14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 397960..409105

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O6P39_RS01870 (O6P39_01870)	-	399302..399592 (-)	291	WP_275609791.1	DUF167 domain-containing protein	-
O6P39_RS01875 (O6P39_01875)	-	399598..400191 (-)	594	WP_275609792.1	YggT family protein	-
O6P39_RS01880 (O6P39_01880)	proC	400205..401038 (-)	834	WP_275609793.1	pyrroline-5-carboxylate reductase	-
O6P39_RS01885 (O6P39_01885)	-	401056..401745 (-)	690	WP_275609794.1	YggS family pyridoxal phosphate-dependent enzyme	-
O6P39_RS01890 (O6P39_01890)	pilT	401819..402853 (+)	1035	WP_151189058.1	type IV pilus twitching motility protein PilT	Machinery gene
O6P39_RS01895 (O6P39_01895)	pilU	402960..404105 (+)	1146	WP_275609795.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
O6P39_RS01900 (O6P39_01900)	-	404198..404602 (+)	405	WP_275609796.1	TM2 domain-containing protein	-
O6P39_RS01905 (O6P39_01905)	-	404828..406201 (+)	1374	WP_275609797.1	cystathionine beta-synthase	-
O6P39_RS01910 (O6P39_01910)	-	406198..407367 (+)	1170	WP_275609798.1	cystathionine gamma-synthase	-
O6P39_RS01915 (O6P39_01915)	-	407651..408922 (-)	1272	WP_275609799.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42605.89 Da
• Isoelectric Point: 6.5779

>NTDB_id=773403 O6P39_RS01895 WP_275609795.1 402960..404105(+) (pilU) [Pseudomonas sp. PSE14]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTFEDLKLPEVLKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHDQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLEMSDDDPSRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=773403 O6P39_RS01895 WP_275609795.1 402960..404105(+) (pilU) [Pseudomonas sp. PSE14]
ATGGAATTCGAGAAGCTGTTGCGCCTCATGGTGGAAAAGGGTGCCTCCGACCTCTTCATCACGGCGGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTCTCGG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCGTTGGCCGT
TTCCGCGTCAGCGCCTTTTATCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCTTCGA
GGATCTGAAGCTTCCCGAAGTCCTCAAGAAGCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATTGGCTACCGCAACAAGAACTCCACCGGCCACATTATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGATTCCTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CGGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCATGACCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTACCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAAGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAAGACGCGCTGGCCTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGCTCGGAAACCGACGCCGACCATCTCTCCACCATGACCCAGGGGCTGACCCTGGAAATGAGCGACG
ACGACCCCAGCCGCCGTTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	89.239	100	0.892
pilU	Acinetobacter baylyi ADP1	63.83	98.688	0.63
pilU	Vibrio cholerae strain A1552	59.569	97.375	0.58
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.617	87.664	0.365
pilT	Vibrio cholerae strain A1552	41.617	87.664	0.365