Detailed information NTG0772737

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PAN99_RS12360
• Genome accession: NZ_CP115604
• Coordinates: 2546481..2547095 (-)
• Length: 204 a.a.
• NCBI ID: WP_043867297.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain FJAT-54560
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2541481..2552095

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAN99_RS12330 (PAN99_12330)	rpsT	2541781..2542047 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PAN99_RS12335 (PAN99_12335)	holA	2542064..2543105 (-)	1042	Protein_2382	DNA polymerase III subunit delta	-
PAN99_RS12340 (PAN99_12340)	-	2543145..2543299 (-)	155	Protein_2383	hypothetical protein	-
PAN99_RS12345 (PAN99_12345)	-	2543340..2543474 (+)	135	WP_003152870.1	YqzM family protein	-
PAN99_RS12350 (PAN99_12350)	comEC	2543493..2545844 (-)	2352	WP_043867296.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PAN99_RS12355 (PAN99_12355)	-	2545845..2546414 (-)	570	WP_003152868.1	ComE operon protein 2	-
PAN99_RS12360 (PAN99_12360)	comEA	2546481..2547095 (-)	615	WP_043867297.1	helix-hairpin-helix domain-containing protein	Machinery gene
PAN99_RS12365 (PAN99_12365)	comER	2547154..2547975 (+)	822	WP_044053476.1	late competence protein ComER	-
PAN99_RS12370 (PAN99_12370)	-	2548044..2548781 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
PAN99_RS12375 (PAN99_12375)	rsfS	2548778..2549134 (-)	357	WP_003152864.1	ribosome silencing factor	-
PAN99_RS12380 (PAN99_12380)	yqeK	2549131..2549712 (-)	582	WP_003152863.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PAN99_RS12385 (PAN99_12385)	-	2549702..2550271 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
PAN99_RS12390 (PAN99_12390)	yhbY	2550282..2550572 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PAN99_RS12395 (PAN99_12395)	aroE	2550566..2551408 (-)	843	WP_025852995.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22178.37 Da
• Isoelectric Point: 6.9685

>NTDB_id=772737 PAN99_RS12360 WP_043867297.1 2546481..2547095(-) (comEA) [Bacillus velezensis strain FJAT-54560]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=772737 PAN99_RS12360 WP_043867297.1 2546481..2547095(-) (comEA) [Bacillus velezensis strain FJAT-54560]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGAAGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363