Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7K57_RS02525 Genome accession   NZ_AP022214
Coordinates   513531..514640 (-) Length   369 a.a.
NCBI ID   WP_182721753.1    Uniprot ID   -
Organism   Aeromonas caviae strain WP8-S17-ESBL-03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508531..519640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K57_RS02500 (WP8S17E03_04670) - 508663..508890 (+) 228 WP_039039956.1 hypothetical protein -
  H7K57_RS02505 (WP8S17E03_04680) brnQ 508981..510243 (-) 1263 WP_050485178.1 branched-chain amino acid transport system II carrier protein -
  H7K57_RS02510 (WP8S17E03_04690) - 510479..511189 (-) 711 WP_103857985.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  H7K57_RS02515 (WP8S17E03_04700) srmB 511317..512537 (+) 1221 WP_182721752.1 ATP-dependent RNA helicase SrmB -
  H7K57_RS02520 (WP8S17E03_04710) yaaA 512725..513498 (-) 774 WP_103857984.1 peroxide stress protein YaaA -
  H7K57_RS02525 (WP8S17E03_04720) pilU 513531..514640 (-) 1110 WP_182721753.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7K57_RS02530 (WP8S17E03_04730) pilT 514664..515698 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  H7K57_RS02535 (WP8S17E03_04740) - 515738..516439 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7K57_RS02540 (WP8S17E03_04750) proC 516563..517387 (+) 825 WP_182721754.1 pyrroline-5-carboxylate reductase -
  H7K57_RS02545 (WP8S17E03_04760) - 517411..517962 (+) 552 WP_010673053.1 YggT family protein -
  H7K57_RS02550 (WP8S17E03_04770) yggU 517962..518261 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  H7K57_RS02555 (WP8S17E03_04780) - 518280..518699 (+) 420 WP_182721755.1 DUF4426 domain-containing protein -
  H7K57_RS02560 (WP8S17E03_04790) - 518775..519605 (-) 831 WP_182721756.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40871.01 Da        Isoelectric Point: 6.3155

>NTDB_id=77174 H7K57_RS02525 WP_182721753.1 513531..514640(-) (pilU) [Aeromonas caviae strain WP8-S17-ESBL-03]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLGPAPLDKTTALALIREGLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=77174 H7K57_RS02525 WP_182721753.1 513531..514640(-) (pilU) [Aeromonas caviae strain WP8-S17-ESBL-03]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCGGACCAGCCCCCCTCGACAAGACGACGGCGCTGGCGCTGATCAGGGAGG
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGGGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCCCCCATTCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTCTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGCCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCATGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.057

0.39
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment