Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCP71_RS01715 Genome accession   NZ_CP115294
Coordinates   365251..366951 (+) Length   566 a.a.
NCBI ID   WP_003120945.1    Uniprot ID   A0A1L7HA63
Organism   Pseudomonas aeruginosa strain 214     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 347064..368172 365251..366951 within 0


Gene organization within MGE regions


Location: 347064..368172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP71_RS01575 (PCP71_01575) - 347064..348278 (+) 1215 WP_023435052.1 site-specific integrase -
  PCP71_RS01580 (PCP71_01580) - 348250..348966 (-) 717 WP_073624880.1 hypothetical protein -
  PCP71_RS01585 (PCP71_01585) - 349115..349315 (+) 201 WP_023435053.1 helix-turn-helix domain-containing protein -
  PCP71_RS01590 (PCP71_01590) - 349312..349728 (+) 417 WP_023435054.1 hypothetical protein -
  PCP71_RS01595 (PCP71_01595) - 349721..349954 (+) 234 WP_023110507.1 hypothetical protein -
  PCP71_RS01600 (PCP71_01600) - 349951..350199 (+) 249 WP_073653397.1 hypothetical protein -
  PCP71_RS01605 (PCP71_01605) - 350196..350501 (+) 306 WP_073653396.1 hypothetical protein -
  PCP71_RS01610 - 350504..350941 (+) 438 WP_305954514.1 hypothetical protein -
  PCP71_RS01615 (PCP71_01615) - 350980..351447 (+) 468 WP_031285502.1 hypothetical protein -
  PCP71_RS01620 (PCP71_01620) - 351454..352407 (+) 954 WP_228294350.1 DUF3631 domain-containing protein -
  PCP71_RS01625 (PCP71_01625) - 352407..352700 (+) 294 WP_023082016.1 hypothetical protein -
  PCP71_RS01630 (PCP71_01630) - 352756..353652 (+) 897 WP_073655669.1 hypothetical protein -
  PCP71_RS01635 (PCP71_01635) - 353783..354196 (+) 414 WP_031629315.1 hypothetical protein -
  PCP71_RS01640 (PCP71_01640) - 354347..354922 (-) 576 WP_124101441.1 hypothetical protein -
  PCP71_RS01645 (PCP71_01645) - 355781..356287 (-) 507 WP_073653394.1 GNAT family N-acetyltransferase -
  PCP71_RS01650 (PCP71_01650) - 357048..357329 (+) 282 WP_033978094.1 hypothetical protein -
  PCP71_RS01655 (PCP71_01655) - 357329..357565 (+) 237 WP_003096116.1 hypothetical protein -
  PCP71_RS01660 (PCP71_01660) - 357772..358053 (-) 282 WP_023435065.1 hypothetical protein -
  PCP71_RS01665 (PCP71_01665) - 358250..358948 (+) 699 WP_124014670.1 hypothetical protein -
  PCP71_RS01670 (PCP71_01670) - 358973..359977 (+) 1005 WP_031689993.1 pesticin C-terminus-like muramidase -
  PCP71_RS01675 (PCP71_01675) - 359990..360652 (+) 663 WP_033992423.1 DUF3218 family protein -
  PCP71_RS01680 (PCP71_01680) fliP 360932..361687 (+) 756 WP_031285507.1 flagellar type III secretion system pore protein FliP -
  PCP71_RS01685 (PCP71_01685) fliQ 361732..362001 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  PCP71_RS01690 (PCP71_01690) fliR 362001..362783 (+) 783 Protein_331 flagellar biosynthetic protein FliR -
  PCP71_RS01695 (PCP71_01695) - 362786..363045 (+) 260 Protein_332 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  PCP71_RS01700 (PCP71_01700) - 364074..364316 (+) 243 WP_023082027.1 hypothetical protein -
  PCP71_RS01710 (PCP71_01710) - 364547..365020 (-) 474 WP_015503553.1 pilin -
  PCP71_RS01715 (PCP71_01715) pilB 365251..366951 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP71_RS01720 (PCP71_01720) pilC 366955..368172 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62385.25 Da        Isoelectric Point: 5.7055

>NTDB_id=771496 PCP71_RS01715 WP_003120945.1 365251..366951(+) (pilB) [Pseudomonas aeruginosa strain 214]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=771496 PCP71_RS01715 WP_003120945.1 365251..366951(+) (pilB) [Pseudomonas aeruginosa strain 214]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L7HA63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.692

100

0.583
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.712

99.293

0.504
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.563

93.463

0.36