Detailed information NTG0768765

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: O9K61_RS12425
• Genome accession: NZ_CP115185
• Coordinates: 2550080..2550694 (-)
• Length: 204 a.a.
• NCBI ID: WP_032866693.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 26.3
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545080..2555694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O9K61_RS12395 (O9K61_12395)	rpsT	2545383..2545649 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
O9K61_RS12400 (O9K61_12400)	holA	2545666..2546707 (-)	1042	Protein_2393	DNA polymerase III subunit delta	-
O9K61_RS12405 (O9K61_12405)	-	2546742..2546898 (-)	157	Protein_2394	hypothetical protein	-
O9K61_RS12410 (O9K61_12410)	-	2546939..2547073 (+)	135	WP_003152870.1	YqzM family protein	-
O9K61_RS12415 (O9K61_12415)	comEC	2547092..2549443 (-)	2352	WP_032866696.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
O9K61_RS12420 (O9K61_12420)	-	2549444..2550013 (-)	570	WP_003152868.1	ComE operon protein 2	-
O9K61_RS12425 (O9K61_12425)	comEA	2550080..2550694 (-)	615	WP_032866693.1	helix-hairpin-helix domain-containing protein	Machinery gene
O9K61_RS12430 (O9K61_12430)	comER	2550753..2551574 (+)	822	WP_032866690.1	late competence protein ComER	-
O9K61_RS12435 (O9K61_12435)	-	2551643..2552380 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
O9K61_RS12440 (O9K61_12440)	rsfS	2552377..2552733 (-)	357	WP_007408260.1	ribosome silencing factor	-
O9K61_RS12445 (O9K61_12445)	yqeK	2552751..2553311 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
O9K61_RS12450 (O9K61_12450)	-	2553301..2553870 (-)	570	WP_032866687.1	nicotinate-nucleotide adenylyltransferase	-
O9K61_RS12455 (O9K61_12455)	yhbY	2553881..2554171 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
O9K61_RS12460 (O9K61_12460)	aroE	2554165..2555007 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22151.28 Da
• Isoelectric Point: 6.4858

>NTDB_id=768765 O9K61_RS12425 WP_032866693.1 2550080..2550694(-) (comEA) [Bacillus velezensis strain 26.3]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSDKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=768765 O9K61_RS12425 WP_032866693.1 2550080..2550694(-) (comEA) [Bacillus velezensis strain 26.3]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGACAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTTAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCATACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.759	100	0.373
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363