Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QPX65_RS00545 Genome accession   NZ_CP126698
Coordinates   119726..121225 (+) Length   499 a.a.
NCBI ID   WP_206638570.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV2018     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 114726..126225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPX65_RS00525 (QPX65_00525) hslO 115118..115993 (-) 876 WP_011078947.1 Hsp33 family molecular chaperone HslO -
  QPX65_RS00530 (QPX65_00530) hslR 116025..116411 (-) 387 WP_085760632.1 ribosome-associated heat shock protein Hsp15 -
  QPX65_RS00535 (QPX65_00535) gspC 116796..117671 (+) 876 WP_157401904.1 type II secretion system protein GspC -
  QPX65_RS00540 (QPX65_00540) gspD 117708..119726 (+) 2019 WP_285455191.1 type II secretion system secretin GspD -
  QPX65_RS00545 (QPX65_00545) pilB/pilB1 119726..121225 (+) 1500 WP_206638570.1 type II secretion system ATPase GspE Machinery gene
  QPX65_RS00550 (QPX65_00550) gspF 121225..122442 (+) 1218 WP_011078942.1 type II secretion system inner membrane protein GspF -
  QPX65_RS00555 (QPX65_00555) gspG 122496..122939 (+) 444 WP_017420501.1 type II secretion system major pseudopilin GspG -
  QPX65_RS00560 (QPX65_00560) gspH 122866..123570 (+) 705 WP_045623808.1 type II secretion system minor pseudopilin GspH -
  QPX65_RS00565 (QPX65_00565) gspI 123563..123925 (+) 363 WP_072610237.1 type II secretion system minor pseudopilin GspI -
  QPX65_RS00570 (QPX65_00570) gspJ 123912..124613 (+) 702 WP_130349475.1 type II secretion system minor pseudopilin GspJ -
  QPX65_RS00575 (QPX65_00575) gspK 124606..125628 (+) 1023 WP_225458102.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55832.11 Da        Isoelectric Point: 6.0826

>NTDB_id=768387 QPX65_RS00545 WP_206638570.1 119726..121225(+) (pilB/pilB1) [Vibrio vulnificus strain VV2018]
MVDILDTAPSYRRLPFSFANRFKMVLEVEHPERPPVLYYVEPLNAQALVEVRRVLKQTFVPQAIASEAFEKKLTEAYQRD
SSEARQLMEDIGSDDDFFSLAEELPQDEDLLEAEDDAPIIKLINAMLGEAIKEGASDIHIETFEKILSIRFRVDGVLRDV
LSPSRKLAPLLVSRVKVMAKLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVVMRLLDKNATRLDLHSLGMTPV
NHDNFRHLISRPHGIILVTGPTGSGKSTTLYAGLQELNSNERNILTVEDPIEFDIDGIGQTQVNPKVDMTFARGLRAILR
QDPDVVMVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAVGAITRLRDMGIEPFLISSSLLGVLAQRLVRTLCQDCKEPY
EADKEQRKLFGAKKKDALTLYRAKGCEKCNHKGYRGRTGIHELLMVDDKVQELIHAEAGEQAMDKYIREHTPSIRSDGLD
KVLQGVTSLEEVMRVTKES

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=768387 QPX65_RS00545 WP_206638570.1 119726..121225(+) (pilB/pilB1) [Vibrio vulnificus strain VV2018]
ATGGTAGATATACTGGATACGGCGCCATCGTATCGCCGTTTACCGTTTAGTTTTGCTAACCGCTTTAAAATGGTGCTGGA
AGTCGAGCATCCAGAAAGGCCGCCGGTACTTTACTACGTTGAGCCTCTCAATGCGCAGGCGTTAGTTGAAGTGCGTCGTG
TGCTCAAACAGACGTTTGTCCCTCAAGCCATTGCCTCTGAAGCGTTTGAAAAAAAACTGACCGAAGCGTACCAACGCGAC
TCGTCAGAAGCGCGCCAGTTGATGGAAGACATTGGCTCGGATGATGACTTCTTCTCTTTGGCTGAAGAGCTGCCGCAAGA
TGAAGATTTGCTGGAAGCGGAAGACGATGCGCCGATCATCAAATTGATCAACGCCATGTTGGGCGAAGCGATCAAAGAAG
GTGCGTCAGATATTCACATCGAAACGTTCGAGAAAATTTTGTCGATTCGTTTCCGCGTCGATGGCGTTTTGCGCGATGTA
CTGTCTCCGAGCCGCAAACTGGCGCCGCTGTTGGTGTCACGTGTTAAAGTCATGGCCAAACTGGATATTGCAGAAAAACG
CGTGCCACAAGATGGTCGTATTTCACTGCGTATCGGTGGTCGCGCGGTTGATGTGCGTGTATCGACCATGCCTTCATCAC
ATGGAGAGCGTGTGGTGATGCGTTTGCTCGACAAAAACGCCACTCGCCTTGACCTACATAGCTTGGGCATGACGCCGGTG
AACCACGACAATTTCCGTCATCTTATTTCTCGTCCTCACGGCATTATTTTGGTCACCGGTCCGACGGGTTCGGGTAAATC
CACCACCTTGTACGCTGGCCTGCAAGAGCTGAACAGCAACGAGCGCAACATCTTAACGGTGGAAGACCCGATCGAATTTG
ATATCGATGGTATCGGCCAAACCCAAGTGAACCCAAAAGTCGACATGACCTTTGCTCGTGGTCTGCGAGCCATTTTGCGT
CAAGACCCAGACGTGGTGATGGTCGGGGAGATTCGTGACTTGGAAACCGCACAAATTGCCGTACAAGCGTCATTGACCGG
TCACTTGGTGATGTCAACCTTGCACACCAACACCGCCGTAGGTGCAATTACCCGTCTGCGTGACATGGGCATTGAGCCCT
TTTTGATCTCTTCTTCACTGCTGGGCGTGTTGGCTCAGCGCCTGGTGCGGACCTTATGCCAAGACTGTAAAGAACCGTAT
GAAGCCGACAAAGAGCAGCGCAAACTGTTTGGCGCGAAGAAGAAAGATGCGCTGACTTTATATCGCGCCAAGGGATGTGA
AAAATGTAACCACAAAGGTTATCGTGGCCGTACGGGTATCCATGAGCTTTTGATGGTGGACGATAAAGTCCAAGAGCTGA
TCCATGCTGAGGCGGGAGAGCAGGCGATGGACAAATACATTCGTGAACATACCCCAAGCATTCGCAGCGACGGTTTAGAC
AAAGTGTTGCAAGGCGTGACGTCACTGGAAGAAGTGATGCGCGTGACCAAGGAATCTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(125-391)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

42.966

100

0.453
  pilF Thermus thermophilus HB27

44.915

94.589

0.425
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.713

98.998

0.423
  pilB Acinetobacter baumannii D1279779

42.292

96.192

0.407
  pilB Legionella pneumophila strain ERS1305867

46.934

84.97

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.348

79.559

0.369
  pilB Vibrio campbellii strain DS40M4

44.853

81.764

0.367
  pilB Vibrio cholerae strain A1552

45.96

79.359

0.365
  pilF Neisseria gonorrhoeae MS11

46.193

78.958

0.365
  pilB Acinetobacter baylyi ADP1

46.41

78.156

0.363