Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4443_RS10995 Genome accession   NZ_CP114551
Coordinates   2384485..2386218 (+) Length   577 a.a.
NCBI ID   WP_088371981.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain OLI17A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379485..2391218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4443_RS10975 (O4443_10975) pilC 2379825..2381027 (-) 1203 WP_282454586.1 type II secretion system F family protein Machinery gene
  O4443_RS10980 (O4443_10980) - 2380957..2381136 (+) 180 WP_282454587.1 hypothetical protein -
  O4443_RS10985 (O4443_10985) - 2381469..2381921 (+) 453 WP_088371979.1 pilin -
  O4443_RS10990 (O4443_10990) - 2383218..2383664 (+) 447 WP_010894963.1 pilin -
  O4443_RS10995 (O4443_10995) pilB 2384485..2386218 (+) 1734 WP_088371981.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4443_RS11000 (O4443_11000) pilR 2386312..2387724 (-) 1413 WP_031338065.1 sigma-54 dependent transcriptional regulator Regulator
  O4443_RS11005 (O4443_11005) - 2387910..2389520 (-) 1611 WP_023907063.1 ATP-binding protein -
  O4443_RS11010 (O4443_11010) sucC 2389770..2390933 (+) 1164 WP_010894968.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63017.12 Da        Isoelectric Point: 6.0946

>NTDB_id=766376 O4443_RS10995 WP_088371981.1 2384485..2386218(+) (pilB) [Xylella fastidiosa subsp. pauca strain OLI17A]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSTAKLAAANALEFGMPLLDVSAFDHN
HNAMRLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKGDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNVSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=766376 O4443_RS10995 WP_088371981.1 2384485..2386218(+) (pilB) [Xylella fastidiosa subsp. pauca strain OLI17A]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAAGATGGCAGCATCGAGGA
AGCCACCGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTGCCGCAGTGGTTGATCGATAAAAAACTAG
TAAGTACCGCAAAGTTGGCCGCAGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTCTCCGCATTCGACCACAAT
CACAATGCAATGCGCTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCACTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAGCAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAGGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACCGGAATTGATGC
CAAGGGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAT
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATGTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTGCTGCGTATCTTGGATGGCAGCGCAGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTATTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGCG
TCAATCAAGTACAACAAAACAACAAACGCGGTATGACGTTTGCCGCAGCATTGCGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGAGACCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAATAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCGCAGCGCATTGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.437

98.267

0.555
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

50.528

98.44

0.497
  pilB Vibrio cholerae strain A1552

49.54

94.107

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

46.903

97.92

0.459
  pilB Vibrio campbellii strain DS40M4

46.561

98.267

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362
  pilF Thermus thermophilus HB27

40.077

89.948

0.36