Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1V13_RS06180 Genome accession   NZ_CP114201
Coordinates   1369793..1371535 (-) Length   580 a.a.
NCBI ID   WP_065345745.1    Uniprot ID   -
Organism   Delftia acidovorans strain BIM B-1761     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1364793..1376535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1V13_RS06150 (O1V13_06150) - 1365358..1365876 (+) 519 WP_269198304.1 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  O1V13_RS06155 (O1V13_06155) - 1365887..1366123 (-) 237 WP_123691054.1 DNA gyrase inhibitor YacG -
  O1V13_RS06160 (O1V13_06160) zapD 1366141..1366896 (-) 756 WP_012207290.1 cell division protein ZapD -
  O1V13_RS06165 (O1V13_06165) coaE 1366986..1367630 (-) 645 WP_269198305.1 dephospho-CoA kinase -
  O1V13_RS06170 (O1V13_06170) - 1367645..1368538 (-) 894 WP_269198306.1 prepilin peptidase -
  O1V13_RS06175 (O1V13_06175) pilC 1368538..1369755 (-) 1218 WP_269198307.1 type II secretion system F family protein Machinery gene
  O1V13_RS06180 (O1V13_06180) pilB 1369793..1371535 (-) 1743 WP_065345745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O1V13_RS06190 (O1V13_06190) - 1371776..1372705 (-) 930 WP_034396917.1 polyprenyl synthetase family protein -
  O1V13_RS06195 (O1V13_06195) rplU 1372936..1373247 (+) 312 WP_012207284.1 50S ribosomal protein L21 -
  O1V13_RS06200 (O1V13_06200) rpmA 1373263..1373520 (+) 258 WP_012207283.1 50S ribosomal protein L27 -
  O1V13_RS06205 (O1V13_06205) cgtA 1373670..1374785 (+) 1116 WP_071956728.1 Obg family GTPase CgtA -
  O1V13_RS06210 (O1V13_06210) proB 1374869..1376017 (+) 1149 WP_269198308.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62759.88 Da        Isoelectric Point: 5.3458

>NTDB_id=765012 O1V13_RS06180 WP_065345745.1 1369793..1371535(-) (pilB) [Delftia acidovorans strain BIM B-1761]
MAVADSSLKNASAVASLPGIGRALISAGKVSQQAAEAAASKAGANRTSFIAELSNAGILSALEVAETVSTMFSAPLLDLD
AVNAARLPHELLDAKIISSYRVLPLSKRGNRITVATADPTQQEAAEQIKFTTQLGVDWIIVEADKLERLIAQQSKSASET
MESYSGGGDFEFGDLKPDEVAEEKDNTAATDVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHQYRVRFRIDGELREIAS
PPIAIKEKLASRIKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KARLIEAIGRPYGMILVTGPTGSGKTVSLYTCLNMLNKAGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKEPAD
IPREALLEAGYDEADIDGSWVTYKPVGCQACNNGYKGRVGIYQVMPISEEIQRIILQDGSALEIAAQAKREGVRSLRGSG
LHKARLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=765012 O1V13_RS06180 WP_065345745.1 1369793..1371535(-) (pilB) [Delftia acidovorans strain BIM B-1761]
ATGGCTGTTGCTGACTCCTCGCTCAAGAATGCTTCCGCTGTTGCATCGCTTCCCGGCATCGGGCGAGCCCTGATATCAGC
AGGCAAGGTATCGCAGCAGGCAGCGGAAGCCGCCGCCAGCAAGGCCGGCGCCAACCGCACCTCGTTCATTGCCGAGTTGA
GCAACGCGGGCATCCTCTCGGCCCTGGAAGTGGCCGAGACGGTCTCCACCATGTTCAGCGCGCCGCTGCTCGATCTTGAT
GCCGTCAACGCCGCGCGCCTGCCGCATGAGTTGCTGGACGCCAAGATCATCAGCTCCTATCGCGTCCTCCCCTTGAGCAA
GCGAGGCAACCGCATCACGGTGGCCACGGCCGACCCGACACAGCAGGAAGCTGCAGAGCAGATCAAGTTCACCACCCAGC
TAGGCGTGGATTGGATCATCGTCGAAGCCGACAAGCTGGAACGCCTGATAGCTCAGCAGAGCAAAAGCGCCAGCGAAACC
ATGGAGAGCTACAGCGGGGGCGGCGATTTCGAGTTCGGCGACCTCAAGCCCGACGAGGTTGCCGAGGAGAAGGACAACAC
GGCGGCAACGGACGTCGAGGACGCGCCCATCGTCAAGTTCCTGCACAAAATGCTGCTCGACGCCTTCAACATGCGCGCGT
CGGACCTGCATTTCGAGCCCTACGAACATCAGTACCGCGTGCGCTTCCGGATTGACGGCGAGTTGCGCGAGATTGCATCG
CCCCCCATTGCGATCAAGGAGAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGCATGGACATCTCGGAAAAGCGGGTTCC
GCAGGACGGGCGCATGAAGCTCAAGGTGGGTCCGGACCGCGTCATCGATTTTCGCGTGAGCACCCTGCCCACGCTCTTCG
GCGAGAAGATCGTGATCCGTATCCTTGACCCGAGCTCGGCCAAGATGGGCATTGATGCCCTGGGCTACGAACCCGAGGAA
AAGGCTCGCCTGATCGAAGCCATCGGGCGCCCCTACGGAATGATTCTGGTGACAGGCCCCACGGGCTCGGGCAAGACGGT
GTCGCTGTACACCTGCCTGAACATGCTGAACAAGGCCGGGGTGAACATCGCGACGGCAGAAGACCCCTCGGAAATCAACC
TGCCCGGCGTCAACCAGGTCAACGTGAACGAAAAGGCAGGCCTCACCTTTGCGACGGCGCTCAAGGCCTTCCTGCGCCAG
GATCCCGACATCATCATGGTCGGTGAAATCCGCGATCTGGAAACGGCCGACATCTCGATCAAGGCCGCGCAGACCGGTCA
CTTGGTGCTGTCCACGCTGCACACCAACGACGCACCGACCACGTTGACGCGCATGCGCAACATGGGCATTGCCCCCTTCA
ACATCGCCTCGAGCGTGATCCTGATCACCGCTCAGCGGCTGGCCAGGCGCCTGTGCCCCCAGTGCAAGGAGCCTGCGGAT
ATCCCGCGGGAGGCGCTGCTGGAGGCGGGCTATGACGAGGCGGACATCGACGGCAGCTGGGTCACCTACAAGCCCGTGGG
CTGCCAGGCATGCAACAACGGCTACAAGGGACGCGTCGGCATCTACCAGGTGATGCCGATCAGCGAGGAAATCCAGCGCA
TCATCCTGCAAGACGGCAGCGCGCTGGAGATCGCGGCACAGGCCAAGCGTGAAGGCGTGCGGTCTCTGCGCGGATCCGGA
CTGCACAAGGCCAGACTAGGACTGACCTCGCTCGAAGAAGTGCTGGCAGTCACCAATGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.712

98.103

0.547
  pilB Acinetobacter baumannii D1279779

56.028

97.241

0.545
  pilF Neisseria gonorrhoeae MS11

52.12

97.586

0.509
  pilB Legionella pneumophila strain ERS1305867

50.264

98.103

0.493
  pilB Vibrio cholerae strain A1552

46.279

95

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

45.372

95

0.431
  pilB Vibrio campbellii strain DS40M4

43.54

97.414

0.424
  pilF Thermus thermophilus HB27

38.028

97.931

0.372