Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OZX61_RS10705 Genome accession   NZ_CP113932
Coordinates   2222046..2223764 (+) Length   572 a.a.
NCBI ID   WP_277094771.1    Uniprot ID   -
Organism   Acinetobacter sp. ESL0695     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2217046..2228764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZX61_RS10670 (OZX61_10670) - 2217341..2218318 (-) 978 WP_277094768.1 aliphatic sulfonate ABC transporter substrate-binding protein -
  OZX61_RS10675 (OZX61_10675) - 2218660..2219019 (-) 360 WP_195770902.1 helix-turn-helix domain-containing protein -
  OZX61_RS10680 (OZX61_10680) - 2219122..2220126 (+) 1005 WP_277095659.1 zinc-binding alcohol dehydrogenase family protein -
  OZX61_RS10685 (OZX61_10685) - 2220139..2220423 (+) 285 WP_277094769.1 putative quinol monooxygenase -
  OZX61_RS10695 (OZX61_10695) secG 2220637..2220960 (-) 324 WP_195770900.1 preprotein translocase subunit SecG -
  OZX61_RS10700 (OZX61_10700) tpiA 2220981..2221775 (-) 795 WP_277094770.1 triose-phosphate isomerase -
  OZX61_RS10705 (OZX61_10705) pilB 2222046..2223764 (+) 1719 WP_277094771.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OZX61_RS10710 (OZX61_10710) pilC 2223787..2225010 (+) 1224 WP_277094772.1 type II secretion system F family protein Machinery gene
  OZX61_RS10715 (OZX61_10715) pilD 2225010..2225867 (+) 858 WP_277094773.1 A24 family peptidase Machinery gene
  OZX61_RS10720 (OZX61_10720) coaE 2225869..2226471 (+) 603 WP_195770895.1 dephospho-CoA kinase -
  OZX61_RS10725 (OZX61_10725) rlmB 2226468..2227211 (-) 744 WP_277094774.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OZX61_RS10730 (OZX61_10730) - 2227321..2227644 (-) 324 WP_195770893.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63728.01 Da        Isoelectric Point: 4.9484

>NTDB_id=763628 OZX61_RS10705 WP_277094771.1 2222046..2223764(+) (pilB) [Acinetobacter sp. ESL0695]
MAPSLVTPRLTGFVRRLVDEGHFSEEKMFDALEKAKKNQEDIVGYLVEKLNFSSLILAEIISKEFGEPLLDLATYDKTLL
PKGLVDTKHILKYRIVPLLQRGNNIIIAISNPTNLEAIDTIRLNSNLQPEIVVVEHNKLLSFIQENFDENAHFNFDDDDL
DISIDENMDTDGGNADDADQDNENEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIATPPLQLANRL
SARLKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYDEDQKALFLDALH
QPQGMVLITGPTGSGKTVSLYTGLNILNTEDSNISTAEDPVEINLEGINQVNVNNKVGLSFATALRSFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLVRKLCPSCKAPIDIPKNSLLEM
GFTEQDLQEDFTIYQPVGCSECREGYKGRLGVYEVMKVTPEISRLIMQDANAIQIADVSEKLGFNNLRRSGLKKVILGLT
SLQEVHRVTSST

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=763628 OZX61_RS10705 WP_277094771.1 2222046..2223764(+) (pilB) [Acinetobacter sp. ESL0695]
ATGGCACCTTCTTTAGTCACACCTCGGTTAACAGGTTTTGTTCGTCGACTCGTCGATGAAGGGCATTTTTCTGAAGAAAA
AATGTTTGATGCTTTAGAAAAAGCAAAAAAAAACCAAGAAGATATTGTGGGGTATCTTGTTGAGAAATTAAATTTTTCTT
CATTGATTCTTGCTGAAATAATTTCTAAAGAGTTTGGGGAACCTCTATTAGATCTTGCTACATACGATAAAACATTACTA
CCAAAAGGCTTAGTAGATACAAAACATATTCTTAAATATCGTATTGTACCACTGTTACAACGTGGTAATAACATTATCAT
TGCCATTAGCAATCCAACCAATCTTGAAGCGATAGATACGATTCGTCTAAACAGTAACTTACAACCAGAAATTGTAGTTG
TAGAACACAATAAATTACTGAGTTTTATTCAAGAAAACTTTGACGAAAACGCACACTTTAATTTTGATGACGATGATCTT
GATATCAGTATTGATGAAAACATGGATACTGATGGTGGGAATGCCGACGATGCTGATCAAGACAATGAAAATGAAGCACC
TATTGTAAAATATATCAACAAATTATTGGTTGATGCAATTCGTATGGGCGCATCAGATTTACACTTCGAGCCTTATGAGA
AAAGCTATCGTGTTCGATATCGTGTAGATGGGGTTCTTCGACAAATTGCAACTCCACCGTTACAACTTGCCAATCGTCTT
TCAGCACGCTTGAAAGTCATGTCTCAGATGGACATTTCTGAGAAACGTGTACCACAAGATGGACGTATTAAGCTTAAACT
CTCTAAAAACAAAGCTATCGATTTTCGTGTGAACTCACTACCAACACTGTTTGGTGAGAAAATTGTATTACGTATTCTAG
ACCCTTCAAGTGCAATGCTAGGTATTGATGCATTAGGTTATGATGAAGACCAAAAAGCATTGTTCTTAGATGCACTTCAT
CAACCTCAGGGAATGGTACTGATTACAGGTCCGACGGGTTCAGGTAAAACTGTTTCCTTATATACAGGCCTAAATATTTT
AAATACTGAAGATTCAAATATTTCAACAGCTGAAGATCCTGTAGAAATTAACTTAGAAGGCATTAACCAAGTTAACGTCA
ACAATAAAGTTGGACTCAGCTTTGCGACAGCACTTAGATCTTTCTTACGTCAAGATCCAGACATTATCATGGTGGGTGAG
ATTCGTGACTTAGAAACAGCAGAGATCGCTATTAAAGCAGCTCAAACTGGTCATATGGTACTGTCAACCCTACATACCAA
TAGCGCACCAGAAACTCTCACGCGCCTAAGAAATATGGGTGTACCATCTTTCAATATTGCAACTTCTGTCAATTTGGTCA
TTGCACAACGTCTCGTTCGTAAACTTTGTCCATCGTGCAAGGCACCTATAGATATCCCTAAAAATAGCTTATTAGAAATG
GGATTTACTGAACAAGATTTACAAGAAGATTTTACGATTTACCAACCTGTGGGTTGCTCTGAGTGTCGTGAAGGCTACAA
AGGTCGTCTAGGTGTTTATGAAGTGATGAAAGTTACACCTGAAATTTCTAGACTAATTATGCAAGACGCAAATGCTATCC
AAATAGCAGATGTTTCTGAAAAATTAGGTTTTAATAATTTAAGACGTTCAGGTCTTAAAAAAGTCATTCTTGGCTTAACA
TCATTACAAGAAGTACATCGCGTCACAAGTAGTACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

74.867

98.776

0.74
  pilB Acinetobacter baylyi ADP1

71.454

99.825

0.713
  pilB Legionella pneumophila strain ERS1305867

50.526

99.65

0.503
  pilB Vibrio campbellii strain DS40M4

50.181

96.853

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.638

96.503

0.479
  pilB Vibrio cholerae strain A1552

51.036

92.832

0.474
  pilF Neisseria gonorrhoeae MS11

51.462

89.685

0.462