Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OXH74_RS03875 Genome accession   NZ_CP113889
Coordinates   835128..836834 (+) Length   568 a.a.
NCBI ID   WP_375740142.1    Uniprot ID   -
Organism   Pseudomonas boanensis strain L337     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830128..841834
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH74_RS03855 (OXH74_03855) - 830465..832705 (-) 2241 WP_375740139.1 DUF1631 domain-containing protein -
  OXH74_RS03860 (OXH74_03860) nadC 833220..834068 (+) 849 WP_375740140.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OXH74_RS03870 (OXH74_03870) - 834487..834900 (-) 414 WP_375740141.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OXH74_RS03875 (OXH74_03875) pilB 835128..836834 (+) 1707 WP_375740142.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OXH74_RS03880 (OXH74_03880) pilC 836837..838054 (+) 1218 WP_375740143.1 type II secretion system F family protein Machinery gene
  OXH74_RS03885 (OXH74_03885) pilD 838057..838929 (+) 873 WP_375740144.1 prepilin peptidase Machinery gene
  OXH74_RS03890 (OXH74_03890) coaE 838926..839537 (+) 612 WP_375740145.1 dephospho-CoA kinase -
  OXH74_RS03895 (OXH74_03895) yacG 839534..839734 (+) 201 WP_375740146.1 DNA gyrase inhibitor YacG -
  OXH74_RS03900 (OXH74_03900) - 840146..840679 (-) 534 WP_375740147.1 transposase -
  OXH74_RS03905 (OXH74_03905) - 840917..841132 (-) 216 WP_375740148.1 hypothetical protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62648.81 Da        Isoelectric Point: 5.7441

>NTDB_id=763338 OXH74_RS03875 WP_375740142.1 835128..836834(+) (pilB) [Pseudomonas boanensis strain L337]
MTETAPTLSGLAKQLVLTNLLDERSALQAHTQAQRNKIPLISYLVQNKLLRSRDLAEMAAEQFGVAFLDLGSVDKDALPR
DLVSEKLIRQHRALPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSATSGMDDLGDVDL
DGVDVQVVDEDKNDGAGGVDADDAPVVRFVNKMLLDAIRSGSSDLHFEPYERIYRVRFRTDGMLHEVAKPPVQLATRIAA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEAEQKELYLAALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCVHCRKELDVPKETLLKEGF
PEDRIGSFKLYGPVGCENCNQGYKGRVGIYEVVKITPAMQHLIMEEGNSIQIAQQARKDGFNDLRTSGLLKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=763338 OXH74_RS03875 WP_375740142.1 835128..836834(+) (pilB) [Pseudomonas boanensis strain L337]
ATGACAGAGACAGCCCCCACCCTTTCTGGCCTGGCCAAACAATTGGTGCTGACCAACCTTCTGGATGAACGGAGCGCCCT
ACAAGCCCATACCCAAGCGCAGCGCAACAAGATTCCACTGATCAGCTACCTGGTGCAGAACAAATTGCTAAGGAGCCGTG
ACCTTGCGGAAATGGCAGCCGAACAGTTCGGCGTGGCATTTCTTGATCTGGGCAGCGTTGATAAAGATGCTCTGCCCAGA
GATCTGGTCAGTGAAAAGCTGATTCGCCAGCACCGCGCCCTGCCTCTCTGGCGTCGCGGCAACAAACTGTTCGTGGGCAT
TTCCGATCCCACCAACCACCAAGCCGTCACTGATATCCAGTTCAGTACCGGGCTGAACACCGAAGCCATTTTGGTGGAAG
ACGATAAGCTCGGTGATGCCATCGAGAAATTCTTCGACAGTGCCACCAGCGGCATGGACGACCTCGGCGATGTCGATCTG
GATGGTGTCGATGTCCAGGTGGTGGACGAGGACAAGAATGATGGGGCGGGTGGGGTCGATGCCGACGATGCTCCGGTAGT
GCGTTTCGTCAACAAGATGCTGTTGGATGCCATTCGCAGCGGCTCGTCCGACCTGCACTTCGAACCCTATGAACGTATTT
ACCGCGTGCGCTTCCGCACTGACGGCATGCTACATGAAGTGGCCAAGCCGCCTGTGCAATTGGCGACACGTATCGCTGCA
CGCCTGAAAGTGATGGCCGCCCTAGATATCTCCGAGCGGCGCAAGCCCCAGGACGGGCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGACTTCCGTGTGAACACCCTACCGACCCTCTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCGA
CCAGCGCACAAATGGGTATCGATGCGCTGGGCTACGAGGCAGAGCAGAAAGAGCTTTACCTCGCGGCGCTGAAACAGCCC
CAGGGGATGATCCTGGTCACCGGCCCCACGGGTTCGGGCAAAACGGTGTCGCTCTATACCGGCCTGAATATCCTCAATAC
CGTGGACATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCA
AACAGGGCCTGGACTTCGCGCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGC
GATCTGGAAACCGCCGAGATCGCCATCAAGGCTGCGCAAACCGGCCACATGGTGCTCTCCACCCTGCACACCAACAGCGC
TGCGGAAACCCTGACACGCCTGCGCAACATGGGCGTACCGGCCTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCC
AGCGTCTGGCACGCAAATTGTGTGTTCACTGCCGGAAGGAGTTGGACGTGCCCAAGGAGACCCTGCTCAAGGAGGGTTTC
CCGGAAGACAGGATTGGCAGCTTCAAGCTCTATGGCCCGGTGGGGTGCGAGAATTGCAATCAGGGCTATAAGGGCCGAGT
GGGGATTTATGAAGTGGTTAAAATCACGCCGGCCATGCAACATCTTATTATGGAAGAAGGAAACTCGATCCAGATTGCCC
AGCAGGCACGCAAAGACGGCTTCAACGACCTGCGCACCTCCGGCCTGCTGAAGGCCATGAACGGCATTACCAGCCTCGAA
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.331

100

0.593
  pilB Acinetobacter baumannii D1279779

58.813

100

0.593
  pilB Legionella pneumophila strain ERS1305867

56.966

99.824

0.569
  pilF Neisseria gonorrhoeae MS11

49.911

99.12

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.468

99.296

0.491
  pilB Vibrio campbellii strain DS40M4

49.201

99.12

0.488
  pilB Vibrio cholerae strain A1552

52.481

92.254

0.484