Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OV592_RS18070 Genome accession   NZ_CP113525
Coordinates   3933078..3934778 (+) Length   566 a.a.
NCBI ID   WP_406609554.1    Uniprot ID   -
Organism   Agarivorans sp. JK6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3928078..3939778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OV592_RS18045 pdhR 3928879..3929643 (-) 765 WP_016400804.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OV592_RS18050 ampE 3929900..3930754 (-) 855 WP_406609550.1 beta-lactamase regulator AmpE -
  OV592_RS18055 ampD 3930829..3931365 (-) 537 WP_406609551.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OV592_RS18060 nadC 3931540..3932379 (+) 840 WP_406609552.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OV592_RS18065 - 3932633..3933073 (+) 441 WP_406609553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OV592_RS18070 pilB 3933078..3934778 (+) 1701 WP_406609554.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OV592_RS18075 pilC 3934866..3936116 (+) 1251 WP_406609555.1 type II secretion system F family protein Machinery gene
  OV592_RS18080 pilD 3936119..3937000 (+) 882 WP_406609556.1 prepilin peptidase Machinery gene
  OV592_RS18085 coaE 3936997..3937599 (+) 603 WP_406609557.1 dephospho-CoA kinase -
  OV592_RS18090 zapD 3937658..3938398 (+) 741 WP_406609558.1 cell division protein ZapD -
  OV592_RS18095 yacG 3938398..3938598 (+) 201 WP_406609559.1 DNA gyrase inhibitor YacG -
  OV592_RS18100 mutT 3938601..3939005 (-) 405 WP_406609560.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62563.91 Da        Isoelectric Point: 4.8571

>NTDB_id=762753 OV592_RS18070 WP_406609554.1 3933078..3934778(+) (pilB) [Agarivorans sp. JK6]
MSSILNGLASSLAVSFPEKEDKLIELYKRSIQEQTAFTTVIVENKFISSKELADYCEAQFGIPLLDLDAIELSEIPQNFL
NEKLIFKHHVLPVYSQNQTLYVAMADPSNVTALEDFGFSFGLHVEALLVEETKLVKAIQALVSDGSDLEIGDISDEDISD
LEVSDESSRLDEKQNEKEDDAPIVVYINKILMDSIRRGASDLHFEPYEHKYRIRFRIDGILHEIASPPINLANRFSARLK
VMARMDIAERRLPQDGRIKLKTGRNKAIDMRVNSLPTMWGEKIVIRILDSSAANLNIDILGYDDKQKKLYLDALARPQGM
ILVTGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLPGVNQVQINNKAGMTFASALRAFLRQDPDIVMVGEIRDLE
TAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLARRLCEKCKKPKMLEAELLPQIGFSQA
QIDAGVKLYEPVGCDECTGGYKGRVGIYEVMPMSSKISKIIMAEGNSIEIAEQAVAEGMYSLRMSALEKARLGVTSLTEV
ERVTNV

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=762753 OV592_RS18070 WP_406609554.1 3933078..3934778(+) (pilB) [Agarivorans sp. JK6]
ATGAGTTCAATATTAAACGGCCTAGCATCAAGCTTGGCCGTTTCTTTTCCAGAAAAAGAAGATAAGCTCATTGAGCTCTA
CAAACGTAGTATTCAAGAGCAAACCGCATTTACTACTGTTATTGTTGAAAATAAATTCATTTCTAGCAAAGAACTTGCAG
ATTATTGTGAGGCTCAGTTTGGCATTCCATTATTAGATTTGGATGCGATAGAACTTAGTGAAATACCTCAGAATTTCCTC
AACGAAAAATTAATATTCAAACATCACGTATTACCAGTTTATAGCCAAAACCAAACTCTTTATGTTGCTATGGCTGACCC
TAGTAATGTCACCGCCCTAGAAGATTTTGGTTTTAGTTTTGGACTGCATGTCGAAGCTTTGCTCGTTGAAGAAACCAAAC
TAGTAAAAGCTATTCAAGCTTTAGTTAGCGATGGTTCGGACTTAGAGATTGGCGATATCTCCGACGAGGATATAAGCGAT
TTAGAGGTTAGTGACGAAAGCTCGCGTTTAGACGAAAAGCAAAACGAAAAAGAAGATGACGCACCGATTGTTGTTTACAT
TAATAAGATATTAATGGACTCCATTCGCCGCGGTGCATCAGATTTACATTTTGAACCTTACGAGCACAAGTACCGGATCC
GCTTTCGCATCGACGGTATTTTGCATGAAATTGCCAGCCCTCCAATTAATTTAGCTAACCGCTTTTCTGCTCGCCTTAAA
GTAATGGCGCGCATGGATATAGCCGAGCGCCGCTTGCCGCAAGATGGCCGCATTAAACTAAAAACTGGCCGCAATAAAGC
CATTGATATGCGGGTTAACTCCCTTCCCACCATGTGGGGAGAGAAGATCGTAATTCGTATTCTTGATTCCTCTGCTGCCA
ACTTAAACATTGATATTCTGGGTTACGACGACAAGCAGAAAAAGCTTTACTTAGATGCTCTCGCTCGCCCACAAGGCATG
ATTCTAGTGACCGGCCCCACCGGCTCAGGTAAAACTGTTTCGCTATATACTGGTTTAAATATTTTAAATACAGTTGAAAC
GAATATTTCAACCGCGGAAGACCCAGTGGAAATTAACTTACCCGGGGTTAATCAAGTACAAATCAATAACAAAGCCGGTA
TGACCTTCGCTAGCGCTTTGCGTGCCTTTCTTCGTCAAGATCCCGATATTGTAATGGTGGGTGAGATTCGGGACTTAGAA
ACAGCCGAGATATCCATTAAAGCGGCTCAAACCGGTCACTTGGTATTATCTACCTTGCACACCAATTCTGCTGCAGAAAC
GCTAACCCGTTTACTTAATATGGGCGTGCCCGCCTTTAATATTGCCTCTTCGGTAAGTTTGATTATTGCTCAACGACTGG
CTAGACGTTTATGTGAAAAATGCAAGAAGCCTAAGATGCTTGAGGCAGAATTACTTCCCCAGATTGGTTTTTCCCAAGCA
CAAATTGATGCCGGAGTTAAATTGTATGAGCCAGTAGGTTGCGATGAATGCACTGGAGGTTATAAGGGGCGAGTAGGTAT
TTATGAAGTCATGCCAATGAGCAGCAAGATATCAAAGATAATCATGGCCGAAGGCAACTCTATTGAAATCGCTGAACAAG
CAGTGGCAGAAGGCATGTATTCCCTTCGTATGTCAGCTTTAGAAAAAGCCCGTTTAGGCGTAACTAGTCTTACAGAGGTA
GAGCGGGTCACAAATGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.852

95.406

0.542
  pilB Legionella pneumophila strain ERS1305867

53.274

99.823

0.532
  pilB Acinetobacter baylyi ADP1

56.084

92.933

0.521
  pilB Vibrio cholerae strain A1552

54.206

94.523

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

52.555

96.82

0.509
  pilB Vibrio campbellii strain DS40M4

51.636

97.173

0.502
  pilF Neisseria gonorrhoeae MS11

48.346

96.113

0.465
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.377

93.64

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.274

94.17

0.36