Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OV592_RS04680 Genome accession   NZ_CP113525
Coordinates   990586..991620 (+) Length   344 a.a.
NCBI ID   WP_016401371.1    Uniprot ID   R9PK19
Organism   Agarivorans sp. JK6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 985586..996620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OV592_RS04645 hemW 985661..986815 (-) 1155 WP_406610575.1 radical SAM family heme chaperone HemW -
  OV592_RS04650 rdgB 986809..987408 (-) 600 WP_406610576.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OV592_RS04655 - 987507..987941 (-) 435 WP_220718831.1 DUF4426 domain-containing protein -
  OV592_RS04660 yggU 988000..988290 (-) 291 WP_016401375.1 DUF167 family protein YggU -
  OV592_RS04665 - 988291..988836 (-) 546 WP_016401374.1 YggT family protein -
  OV592_RS04670 proC 988854..989675 (-) 822 WP_406610577.1 pyrroline-5-carboxylate reductase -
  OV592_RS04675 - 989862..990560 (-) 699 WP_406610578.1 YggS family pyridoxal phosphate-dependent enzyme -
  OV592_RS04680 pilT 990586..991620 (+) 1035 WP_016401371.1 type IV pilus twitching motility protein PilT Machinery gene
  OV592_RS04685 pilU 991629..992747 (+) 1119 WP_016401370.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OV592_RS04690 yaaA 992765..993538 (+) 774 WP_016401369.1 peroxide stress protein YaaA -
  OV592_RS04695 - 993700..994167 (+) 468 WP_406610579.1 hypothetical protein -
  OV592_RS04700 - 994376..995119 (+) 744 WP_406610580.1 OmpA family protein -
  OV592_RS04705 srmB 995182..996438 (-) 1257 WP_406610581.1 ATP-dependent RNA helicase SrmB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38335.92 Da        Isoelectric Point: 6.3901

>NTDB_id=762739 OV592_RS04680 WP_016401371.1 990586..991620(+) (pilT) [Agarivorans sp. JK6]
MDVTELLAFSVKHNASDLHLSAELPPMIRVDGEVRKINVPALEHKQVHSLIYDIMNDKQRKEYEENLEIDFSFEVPGVAR
FRVNAFQQSRGAAAVFRTIPSEVLSLEQLGAPAIFRQIAEHPRGLVLVTGPTGSGKSTTLAAMIDYINEQRHEHILTIED
PIEFVHKNKNCLINQREVHRDTHSFNNALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKDMVRSMLSESLRAVIAQTLLKKLGGGRVAAHEIMTGIPAIRNLIREDKVAQMYSVIQTGMAHGMQTLDQNL
KELVNQGMVSYEDAKLKAADPNNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=762739 OV592_RS04680 WP_016401371.1 990586..991620(+) (pilT) [Agarivorans sp. JK6]
ATGGATGTTACTGAATTACTGGCCTTTAGTGTAAAACATAACGCGTCGGATCTACACCTATCTGCAGAATTGCCACCGAT
GATTCGGGTAGACGGCGAAGTGCGTAAAATTAACGTACCGGCATTAGAGCACAAACAAGTTCATAGTCTGATCTACGACA
TTATGAACGATAAGCAACGTAAAGAGTATGAAGAAAACCTCGAGATTGACTTCTCGTTTGAGGTGCCCGGCGTAGCGCGC
TTTCGTGTGAACGCCTTCCAACAAAGCCGCGGCGCAGCAGCGGTATTTCGTACCATTCCTTCAGAAGTATTGAGTTTAGA
GCAACTAGGTGCACCGGCCATTTTTAGGCAAATTGCTGAACACCCACGTGGTTTAGTGCTAGTAACTGGCCCAACCGGTT
CTGGTAAATCAACCACATTGGCGGCGATGATCGACTACATTAATGAGCAACGTCACGAGCACATCCTTACCATTGAAGAC
CCGATAGAATTTGTGCACAAAAACAAAAACTGTCTGATTAACCAACGTGAAGTGCACCGCGATACCCACAGCTTTAACAA
TGCGCTACGTTCAGCGCTTCGTGAAGACCCGGATATTATTCTGGTTGGTGAATTACGTGACTTAGAAACCATTCGTTTAG
CACTCACCGCAGCCGAAACCGGTCACTTAGTATTTGGCACTTTGCACACCACCTCAGCAGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCAGCAGCAGAAAAAGACATGGTACGTTCCATGTTGTCGGAATCGCTGCGTGCGGTAATTGCTCAAAC
TCTGCTTAAAAAACTGGGTGGCGGACGAGTGGCTGCTCACGAAATCATGACCGGTATTCCAGCGATTCGTAACTTGATTC
GTGAAGATAAAGTAGCGCAAATGTATTCGGTTATTCAAACCGGTATGGCGCACGGCATGCAGACCTTGGATCAAAACTTA
AAAGAGTTGGTGAACCAAGGGATGGTGTCGTATGAAGACGCTAAACTTAAAGCTGCAGATCCGAATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R9PK19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Pseudomonas stutzeri DSM 10701

78.488

100

0.785
  pilT Pseudomonas aeruginosa PAK

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.074

97.965

0.735
  pilT Vibrio cholerae strain A1552

75.074

97.965

0.735
  pilT Neisseria meningitidis 8013

68.328

99.128

0.677
  pilT Neisseria gonorrhoeae MS11

68.035

99.128

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Vibrio cholerae strain A1552

41.57

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404