Detailed information NTG0762740

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OV592_RS04685
• Genome accession: NZ_CP113525
• Coordinates: 991629..992747 (+)
• Length: 372 a.a.
• NCBI ID: WP_016401370.1
• Uniprot ID: R9PJR7
• Organism: Agarivorans sp. JK6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 986629..997747

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OV592_RS04650	rdgB	986809..987408 (-)	600	WP_406610576.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
OV592_RS04655	-	987507..987941 (-)	435	WP_220718831.1	DUF4426 domain-containing protein	-
OV592_RS04660	yggU	988000..988290 (-)	291	WP_016401375.1	DUF167 family protein YggU	-
OV592_RS04665	-	988291..988836 (-)	546	WP_016401374.1	YggT family protein	-
OV592_RS04670	proC	988854..989675 (-)	822	WP_406610577.1	pyrroline-5-carboxylate reductase	-
OV592_RS04675	-	989862..990560 (-)	699	WP_406610578.1	YggS family pyridoxal phosphate-dependent enzyme	-
OV592_RS04680	pilT	990586..991620 (+)	1035	WP_016401371.1	type IV pilus twitching motility protein PilT	Machinery gene
OV592_RS04685	pilU	991629..992747 (+)	1119	WP_016401370.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OV592_RS04690	yaaA	992765..993538 (+)	774	WP_016401369.1	peroxide stress protein YaaA	-
OV592_RS04695	-	993700..994167 (+)	468	WP_406610579.1	hypothetical protein	-
OV592_RS04700	-	994376..995119 (+)	744	WP_406610580.1	OmpA family protein	-
OV592_RS04705	srmB	995182..996438 (-)	1257	WP_406610581.1	ATP-dependent RNA helicase SrmB	-
OV592_RS04710	-	996536..997258 (+)	723	WP_406610582.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41606.76 Da
• Isoelectric Point: 6.0915

>NTDB_id=762740 OV592_RS04685 WP_016401370.1 991629..992747(+) (pilU) [Agarivorans sp. JK6]
MLLDELLSDMSERKASDLYLSVGVPPSAKVSGRLMPLNEQKLMPEHVMDMLAAIRTEAQLEAFVTGREANFAIARQGLGR
YRVSAFYQREQPSMVIRRIETDIPSFEQLQLPQILKEVGMSKRGLILFVGATGAGKSTTQASMIDYRNHNSSGHILTIED
PVEFMHKHAGCIVNQREVGIDTPSFDEALKNSLRQAPDVILIGEIRTRETMEFALQFSETGHLCMATLHANNANQALDRI
MHLVPEERHRQLCFDLSFNLRAIVAQQLVPTKDGNGRRGVFEILLNTPLAADLIRKGDMHKLKELMSKSREQGMLTFDQS
LFELFESGVIGYTEAIAHADSPNDLRLMIKLHGTEKSNNLDSGMLDGVTVDF

Nucleotide

• Length: 1119 bp

>NTDB_id=762740 OV592_RS04685 WP_016401370.1 991629..992747(+) (pilU) [Agarivorans sp. JK6]
ATGTTACTCGATGAGCTATTAAGCGATATGTCGGAGCGTAAAGCATCCGATTTATATCTATCGGTTGGGGTCCCGCCTAG
CGCTAAAGTGAGTGGCCGTTTAATGCCGCTAAATGAGCAAAAGCTAATGCCAGAGCATGTAATGGATATGCTAGCCGCCA
TTCGCACAGAGGCTCAGCTAGAAGCTTTTGTTACTGGTCGTGAGGCAAACTTTGCCATCGCTCGCCAAGGCTTGGGCCGT
TACCGTGTGAGTGCTTTTTATCAACGCGAACAGCCAAGCATGGTAATTCGTCGTATTGAAACTGATATTCCAAGTTTTGA
GCAGCTGCAGTTGCCGCAAATTCTCAAAGAAGTAGGTATGTCGAAACGTGGTTTGATTTTGTTTGTTGGTGCGACCGGTG
CGGGTAAGTCAACCACTCAGGCCAGTATGATCGATTACCGTAATCACAATTCCAGTGGGCATATTCTTACCATTGAAGAC
CCGGTGGAGTTTATGCACAAACATGCTGGTTGTATTGTTAACCAGCGTGAAGTGGGCATTGACACGCCATCGTTTGATGA
AGCTTTGAAGAACTCATTGCGTCAAGCACCCGATGTGATTTTGATTGGTGAGATCCGTACCCGCGAGACCATGGAGTTTG
CGTTGCAATTCTCTGAAACGGGTCACTTATGTATGGCTACCTTGCACGCCAACAACGCGAACCAAGCTCTAGACCGGATT
ATGCACTTGGTGCCAGAAGAGCGTCATCGCCAGTTGTGTTTTGACTTATCATTCAACCTACGTGCGATTGTGGCTCAGCA
GTTAGTGCCAACCAAAGATGGTAACGGCCGTCGCGGGGTATTTGAAATTTTACTTAATACACCATTAGCTGCCGATTTAA
TTCGTAAAGGTGATATGCATAAGCTCAAAGAGTTAATGAGTAAATCTCGCGAACAGGGCATGTTAACCTTCGACCAAAGC
TTGTTTGAATTGTTCGAAAGTGGCGTAATTGGCTATACCGAAGCTATTGCCCATGCTGATTCACCTAACGATCTTCGTTT
GATGATTAAATTGCATGGCACCGAAAAATCGAATAATTTAGACAGCGGAATGTTAGACGGTGTTACCGTCGATTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

3D structure

• AlphaFold DB: R9PJR7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.14	100	0.591
pilU	Vibrio cholerae strain A1552	56.452	100	0.565
pilU	Acinetobacter baylyi ADP1	57.66	96.505	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.691	92.204	0.384