Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NM686_RS21200 Genome accession   NZ_CP113517
Coordinates   4488593..4489750 (-) Length   385 a.a.
NCBI ID   WP_255189795.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4483593..4494750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS21185 (NM686_021185) bstA 4484079..4486796 (+) 2718 WP_255189792.1 sterol transporter cytoplasmic membrane protein BstA -
  NM686_RS21190 (NM686_021190) bstB 4486796..4487635 (+) 840 WP_255189793.1 sterol transporter periplasmic substrate-binding protein BstB -
  NM686_RS21195 (NM686_021195) bstC 4487673..4488440 (+) 768 WP_255189794.1 sterol transporter outer membrane protein BstC -
  NM686_RS21200 (NM686_021200) pilU 4488593..4489750 (-) 1158 WP_255189795.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NM686_RS21205 (NM686_021205) - 4489882..4490925 (+) 1044 WP_255189796.1 hypothetical protein -
  NM686_RS21210 (NM686_021210) - 4490922..4491197 (+) 276 WP_255189797.1 sulfurtransferase TusA family protein -
  NM686_RS21215 (NM686_021215) argJ 4491221..4492435 (+) 1215 WP_255189798.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  NM686_RS21220 (NM686_021220) - 4492436..4493368 (+) 933 WP_255189799.1 Nudix family hydrolase -
  NM686_RS21225 (NM686_021225) yacG 4493365..4493577 (-) 213 WP_255189800.1 DNA gyrase inhibitor YacG -
  NM686_RS21230 (NM686_021230) zapD 4493577..4494347 (-) 771 WP_255189801.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42607.66 Da        Isoelectric Point: 5.8108

>NTDB_id=762658 NM686_RS21200 WP_255189795.1 4488593..4489750(-) (pilU) [Methylomonas rapida strain MP1]
MEFKDYLKILVQHDGSDLYLTNGAPPAAKFQGTLKPIENIKLTNERLKEIANAIMDNDQRSSFEHVPEMNLAISEPGIGR
FRVNIFKQRNSYALVIRNIKVDIPNADVLGLPQILKDKIMEKRGLILFVGGTGSGKSTSLAALIDHRNSNSSGHIITIED
PIEYVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLLDLSLNLQAFVSQRLVPTVDGKRVAAIEILLGTKLVSDLIQKGEVHAIKEAMEKSENIGMQTFDSHL
LKLYKSGVISLEEALRNSDSPNNLKLKINLSEGLGSATAPKAGGLLDKLALEEISKEEDESEEGQ

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=762658 NM686_RS21200 WP_255189795.1 4488593..4489750(-) (pilU) [Methylomonas rapida strain MP1]
ATGGAATTCAAAGATTATCTGAAAATATTGGTGCAGCACGACGGTTCCGACCTTTATTTGACCAACGGAGCGCCACCCGC
CGCCAAATTCCAAGGCACCTTGAAGCCTATAGAAAACATCAAGTTGACCAACGAGCGCCTGAAAGAAATCGCCAACGCCA
TTATGGACAACGATCAGCGCAGCAGCTTCGAACATGTACCGGAAATGAATCTGGCCATTTCCGAGCCCGGCATCGGCCGT
TTTCGGGTCAATATTTTCAAGCAGCGCAACTCCTATGCTTTGGTGATACGTAACATCAAGGTCGACATTCCCAATGCCGA
TGTACTCGGTTTGCCGCAGATACTGAAAGACAAGATCATGGAAAAACGCGGACTGATTCTGTTCGTCGGCGGCACGGGTT
CCGGCAAATCCACCTCGCTGGCGGCCTTGATCGACCATCGCAACAGCAACTCGTCCGGGCACATCATTACGATCGAAGAC
CCGATCGAATACGTGCATCCGCATAAAAAATCACTGGTCAACCAGCGCGAAGTCGGCGTCGATACCTTGAGTTACGAGGA
TGCGTTGAAAAACACCCTGCGCCAGGCACCGGACGTGATTTTGATCGGCGAGATTCGCAGTCAGGAAACCATGGAACATG
CCCTGGCCTTCGCCGAAACCGGCCATTTGTGCCTGTCGACCTTGCACGCCAACAACGCCAACCAGGCCTTGGATCGAATC
ATCAATTTCTTTCCGGAGGAGCGCCGCAATCAATTGCTGTTGGATTTGTCGCTGAATCTGCAAGCCTTCGTTTCACAGCG
CCTGGTGCCAACCGTCGATGGCAAGCGTGTCGCGGCGATCGAAATTTTGCTGGGCACCAAACTGGTCAGCGATTTGATCC
AGAAAGGCGAGGTGCACGCGATCAAGGAGGCCATGGAAAAATCCGAGAATATCGGCATGCAGACCTTCGACAGCCATTTG
CTGAAGCTTTACAAAAGCGGCGTCATTTCATTGGAAGAAGCCCTGCGCAACTCCGACTCGCCCAACAACCTGAAATTAAA
GATCAATCTCAGCGAAGGACTGGGCTCGGCCACCGCCCCGAAAGCCGGAGGCTTGCTGGATAAGCTGGCGCTGGAAGAGA
TTAGCAAAGAGGAGGATGAAAGCGAAGAAGGCCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.12

91.169

0.53
  pilU Acinetobacter baylyi ADP1

53.955

91.948

0.496
  pilU Vibrio cholerae strain A1552

49.153

91.948

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.659

94.545

0.384
  pilT Legionella pneumophila strain Lp02

42.121

85.714

0.361
  pilT Legionella pneumophila strain ERS1305867

42.121

85.714

0.361