Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   H7X73_RS02735 Genome accession   NZ_AP022013
Coordinates   555055..556164 (-) Length   369 a.a.
NCBI ID   WP_010673049.1    Uniprot ID   A0AAJ6CRB8
Organism   Aeromonas caviae strain WP3-S18-ESBL-02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 550055..561164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7X73_RS02710 (WP3S18E02_05190) - 550188..550415 (+) 228 WP_039039956.1 hypothetical protein -
  H7X73_RS02715 (WP3S18E02_05200) brnQ 550505..551812 (-) 1308 WP_039040015.1 branched-chain amino acid transport system II carrier protein -
  H7X73_RS02720 (WP3S18E02_05210) - 552003..552713 (-) 711 WP_183149273.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  H7X73_RS02725 (WP3S18E02_05220) srmB 552841..554061 (+) 1221 WP_183149274.1 ATP-dependent RNA helicase SrmB -
  H7X73_RS02730 (WP3S18E02_05230) yaaA 554249..555022 (-) 774 WP_042014926.1 peroxide stress protein YaaA -
  H7X73_RS02735 (WP3S18E02_05240) pilU 555055..556164 (-) 1110 WP_010673049.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  H7X73_RS02740 (WP3S18E02_05250) pilT 556188..557222 (-) 1035 WP_010673050.1 type IVa pilus ATPase TapT Machinery gene
  H7X73_RS02745 (WP3S18E02_05260) - 557262..557963 (+) 702 WP_042864460.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7X73_RS02750 (WP3S18E02_05270) proC 558085..558909 (+) 825 WP_183149275.1 pyrroline-5-carboxylate reductase -
  H7X73_RS02755 (WP3S18E02_05280) - 558933..559484 (+) 552 WP_042014922.1 YggT family protein -
  H7X73_RS02760 (WP3S18E02_05290) yggU 559484..559783 (+) 300 WP_010673054.1 DUF167 family protein YggU -
  H7X73_RS02765 (WP3S18E02_05300) - 559802..560221 (+) 420 WP_049636470.1 DUF4426 domain-containing protein -
  H7X73_RS02770 (WP3S18E02_05310) - 560297..561127 (-) 831 WP_183149276.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41000.18 Da        Isoelectric Point: 6.4409

>NTDB_id=76209 H7X73_RS02735 WP_010673049.1 555055..556164(-) (pilU) [Aeromonas caviae strain WP3-S18-ESBL-02]
MNMDVLLAALVEQKGSDLFITVDAPPTLKVNGRLVSLRPAPLDKTTALALIRESLDETHFERFLHTREANYAIQRGSLGR
FRVSAFWQQDMPGMVLRRIETRIPTFDELVLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHGDGHILTVED
PVEFVHQHDRCLVTQREVGIDTASFDVALKNSLRQAPDVILIGEIRSQETMEFAIQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSAHGQRRVAAFEILLNTPLVTDIIRKGEMHRLKEVMTKSGELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=76209 H7X73_RS02735 WP_010673049.1 555055..556164(-) (pilU) [Aeromonas caviae strain WP3-S18-ESBL-02]
ATGAACATGGATGTTCTGCTCGCCGCACTGGTTGAACAAAAGGGCTCGGATCTCTTCATCACGGTCGATGCCCCCCCGAC
CCTCAAGGTCAACGGGCGTCTGGTGTCGCTCAGGCCGGCTCCCCTCGACAAGACGACGGCGCTGGCGTTGATCAGGGAGA
GCCTGGACGAGACCCACTTCGAGCGCTTCCTGCACACCCGCGAGGCCAACTACGCCATCCAGCGAGGCTCGCTGGGGCGC
TTTCGGGTCAGCGCCTTCTGGCAACAGGATATGCCGGGCATGGTGCTGCGCCGCATCGAGACCCGCATCCCCACCTTTGA
CGAGCTGGTGCTGCCTCCCATCCTGCAGGAGGTCGCCATGGCCAAACGCGGGCTGGTGCTTTTCGTCGGCGCCACTGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATAGGCTTTCGCAACCAGCACGGTGACGGCCACATCCTGACGGTGGAGGAT
CCGGTGGAGTTCGTCCATCAGCACGATCGCTGTCTGGTGACCCAGCGGGAGGTGGGGATCGACACCGCATCCTTCGATGT
CGCCCTCAAAAACTCCCTGCGCCAGGCCCCGGATGTCATCCTCATCGGTGAAATCCGCTCCCAGGAGACCATGGAGTTCG
CCATCCAGTTCGCCGAGACGGGGCACCTCTGCCTCGCCACCTTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTCCCCCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
GCTGCTCCCCTCCGCTCACGGGCAGCGCCGGGTCGCCGCCTTCGAGATCCTGCTCAACACCCCGCTCGTCACCGACATCA
TCCGCAAGGGAGAGATGCACAGACTCAAGGAGGTGATGACCAAATCCGGCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGCGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTCATCAAGCTGTCGGGTCGCGAGCAGCTGGGCGCAGGCACTCTCGACAACGTGACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.56

91.057

0.388
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379

Multiple sequence alignment