Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   OU819_RS05775 Genome accession   NZ_CP113428
Coordinates   1120853..1124368 (+) Length   1171 a.a.
NCBI ID   WP_078438419.1    Uniprot ID   -
Organism   Bacillus cereus strain NW6     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1115853..1129368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU819_RS05755 (OU819_05755) - 1117313..1117972 (+) 660 WP_000573662.1 response regulator transcription factor -
  OU819_RS05760 (OU819_05760) - 1118246..1118827 (+) 582 WP_000727687.1 hypothetical protein -
  OU819_RS05765 (OU819_05765) - 1119523..1120116 (+) 594 WP_000347516.1 TVP38/TMEM64 family protein -
  OU819_RS05770 (OU819_05770) lepB 1120173..1120736 (+) 564 WP_000751900.1 signal peptidase I -
  OU819_RS05775 (OU819_05775) addB 1120853..1124368 (+) 3516 WP_078438419.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  OU819_RS05780 (OU819_05780) addA 1124365..1128090 (+) 3726 WP_070172158.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  OU819_RS05785 (OU819_05785) - 1128105..1128392 (+) 288 WP_070172157.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  OU819_RS05790 (OU819_05790) gerPF 1128430..1128645 (-) 216 WP_001141566.1 spore germination protein GerPF -
  OU819_RS05795 (OU819_05795) - 1128688..1129074 (-) 387 WP_000902315.1 spore germination protein GerPE -
  OU819_RS05800 (OU819_05800) gerPD 1129090..1129284 (-) 195 WP_001052802.1 spore germination protein GerPD -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134265.36 Da        Isoelectric Point: 5.2869

>NTDB_id=761895 OU819_RS05775 WP_078438419.1 1120853..1124368(+) (addB) [Bacillus cereus strain NW6]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGETILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVKGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTIHTAANL
RAEVEGVAREIRRLVAEENYRYRDVAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMNLLDTFVEMLGEEK
MSLSMFTDVMSTGLDALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSAAGIEL
APTTRQTLLEEQFIMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSTVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGHAASPAGVLYFHIHNPIVEVKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=761895 OU819_RS05775 WP_078438419.1 1120853..1124368(+) (addB) [Bacillus cereus strain NW6]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAAGAAGAGTTAAA
GCAGCGTCCAAGAGGGGAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTTAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAAGTATTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTATTCCAAAAAGCAGCGGAGCAAAATGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGATATA
ACGTGACGCCATCAAACGTATATGAAATGTGGCAACAATTAGATGCACATAGTAGCAGTGCTGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAGTACTTAGATTCAGAAGACTA
TCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAATATGTAAAGGGAGCTGAAATTTATATAGATGGCTTCCATTCGT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGAGCGAGAGTGACAATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACGATTCCGCTTATGGAACAACCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAGAAATTTAACGGGGAAGCAAGTGTAACGATTCATACAGCTGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCTCGTGAAATTCGTAGACTTGTGGCTGAAGAAAACTATCGTTACCGAGATGTTGCGGT
TCTTCTTCGTAACGGGGAAAGTTATTACGATGTAATGCGGACACTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGTTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGGAATTGGCGTTAT
GATGCAGTGTTTCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTATTGCTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAAGATCCGTGGATGTATCGTCGCT
ATCGTTCTCTTGACGATACGAACGGGATGATCACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATCGATTGCGTGAC
GTTGTAAGAACGCCAGTTATTCGTATGCAAAAAAGACTGAAGCGCGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
CTTATTTTTAGAAGAACTGGACGTTCCAAAAAAATTAGAAGCATTACGTATTCGTGCAGAAGAGAGTGGGGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTTATGAATCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGATGCGCTTCAATTCGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTGATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTTATTGGCGTGAATGAAGGTG
TCATTCCAGCAGCACCGATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGATGTTTTGAGCGCTGCAGGTATTGAACTC
GCACCAACGACGAGACAAACTTTATTAGAAGAACAGTTCATTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACGAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCG
GAAGTAACACTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGACTTTTGGTG
GGATGTATATAATTTCTACGTAACTTCGGATGAATGGAAGCAAAAAAGTAGCCGCGTATTATCAAGTTTATTTTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGCTGTAAGTAGAGATTTATACGGAGACAAAATAAAAGGAAGCGTTTCTCGTATGGAA
CTATTTAACCGTTGTGCGTACGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGATATTTTTAAACTTGATGC
GCCAGATATCGGGGAGCTTTTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAATCGTACTTGGGCAG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCTACTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAACCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGCACGTCAATCATTCTTCGTGAACA
TGCGAAGTCTAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTTGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCGTTACCTAATGGTGTAAAGATGGAAGTAGTTGGCCGTATTGACCGCGTTGATAAGGCAGAAGATGAAAACGGA
ACATTCCTCCGTATTATTGACTATAAATCGAGCTCAAAGGCGTTAGACTTAACAGAAGTGTATTACGGGTTGGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCCATGCAGCATCACCAGCGGGTG
TATTGTATTTCCACATTCATAACCCAATTGTTGAGGTGAAAGGTGACGCATCTGAAGCAGAAATTGAAAAGGAAATTTTA
AAGAAATTCAAAATGAAAGGTCTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAATAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAACAAGAGTTTA
ACGTCCTGCAAAAATATGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGTGTGATCGATATTGCCCCT
TACAAAAAAGGAAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCGGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAATTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.317

100

0.494