Detailed information NTG0761336

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: OUY36_RS05055
• Genome accession: NZ_CP113258
• Coordinates: 1083245..1084948 (+)
• Length: 567 a.a.
• NCBI ID: WP_267937009.1
• Uniprot ID: -
• Organism: Stutzerimonas sp. R40042
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1080139..1094997	1083245..1084948	within	0

Gene organization within MGE regions

Location: 1080139..1094997

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OUY36_RS05035 (OUY36_05035)	-	1080139..1080975 (-)	837	WP_267937006.1	hypothetical protein	-
OUY36_RS05040 (OUY36_05040)	-	1080977..1081786 (-)	810	WP_267937007.1	ABC transporter permease subunit	-
OUY36_RS05045 (OUY36_05045)	-	1082207..1082662 (-)	456	WP_267937008.1	hypothetical protein	-
OUY36_RS21985	-	1082692..1082877 (-)	186	WP_272890827.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
OUY36_RS05055 (OUY36_05055)	pilB	1083245..1084948 (+)	1704	WP_267937009.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OUY36_RS05060 (OUY36_05060)	pilC	1084951..1086171 (+)	1221	WP_063544459.1	type II secretion system F family protein	Machinery gene
OUY36_RS05065 (OUY36_05065)	pilD	1086175..1087041 (+)	867	WP_267937010.1	A24 family peptidase	Machinery gene
OUY36_RS05070 (OUY36_05070)	coaE	1087174..1087782 (+)	609	WP_267937011.1	dephospho-CoA kinase	-
OUY36_RS05075 (OUY36_05075)	yacG	1087779..1087976 (+)	198	WP_102840588.1	DNA gyrase inhibitor YacG	-
OUY36_RS05080 (OUY36_05080)	-	1088001..1088681 (-)	681	WP_102840589.1	energy-coupling factor ABC transporter permease	-
OUY36_RS05085 (OUY36_05085)	-	1088720..1089757 (-)	1038	WP_267937013.1	NADP(H)-dependent aldo-keto reductase	-
OUY36_RS05090 (OUY36_05090)	rplM	1090199..1090627 (+)	429	WP_063544471.1	50S ribosomal protein L13	-
OUY36_RS05095 (OUY36_05095)	rpsI	1090642..1091034 (+)	393	WP_013981948.1	30S ribosomal protein S9	-
OUY36_RS05100 (OUY36_05100)	petA	1091289..1091882 (+)	594	WP_156244357.1	ubiquinol-cytochrome c reductase iron-sulfur subunit	-
OUY36_RS05105 (OUY36_05105)	-	1091882..1093093 (+)	1212	WP_110777978.1	cytochrome bc complex cytochrome b subunit	-
OUY36_RS05110 (OUY36_05110)	-	1093093..1093872 (+)	780	WP_267937018.1	cytochrome c1	-
OUY36_RS05115 (OUY36_05115)	-	1093961..1094578 (+)	618	WP_110777975.1	glutathione S-transferase N-terminal domain-containing protein	-
OUY36_RS05120 (OUY36_05120)	-	1094593..1094997 (+)	405	WP_267937020.1	ClpXP protease specificity-enhancing factor	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62328.31 Da
• Isoelectric Point: 5.3387

>NTDB_id=761336 OUY36_RS05055 WP_267937009.1 1083245..1084948(+) (pilB) [Stutzerimonas sp. R40042]
MNENAPLGGLARQLVLLGQLDEKIAQQAQQQALRNQVPLVTWLVQNKLVKSRVLVDIAGEQFGVPFFDLGTLDKETQPKE
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARRDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=761336 OUY36_RS05055 WP_267937009.1 1083245..1084948(+) (pilB) [Stutzerimonas sp. R40042]
ATGAACGAAAACGCGCCCCTTGGCGGCTTGGCCCGGCAGCTGGTTCTCTTAGGCCAGCTCGACGAAAAAATCGCACAGCA
GGCCCAGCAGCAAGCCTTGCGTAACCAAGTGCCTTTGGTGACTTGGTTGGTGCAGAACAAGCTGGTCAAAAGTCGGGTGC
TTGTCGATATCGCCGGTGAGCAGTTTGGTGTTCCGTTCTTTGATTTAGGGACGCTGGACAAAGAAACCCAGCCGAAGGAA
CTGGTCAGCGAGAAGCTGGTGCGCCAGCACCGAGCTCTGCCGTTGCAGCGACGAGGCAACAAGCTTTATGTGGCGGTGTC
CGATCCAACCAACCATCAGGCGATCAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTTGAAGGGTTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGCGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCGGATGATGCGCCAGTGGTACG
GTTTGTTAACAAGATGCTGCTCGATGCGATTAAGGGTGGTTCGTCCGATTTGCACTTCGAGCCTTATGAAAAGAGCTATC
GGGTGCGCTTTCGTACGGACGGCATGTTGCACGAGGTTGCTCGCCCGCCGGTCCAGTTGGTCAGCCGTATCTCAGCGCGT
CTGAAAGTAATGGCCAGTCTGGATATCGCCGAGCGGCGCAAGCCGCAGGACGGACGGATCAAGATGAAGGTCTCGAGCAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACTCTGTGGGGCGAGAAGATCGTTATGCGCATCTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGTTGGGGTATGAGGATGACCAGAAGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGCCCGACAGGCTCGGGCAAGACTGTCTCGCTTTATACCGGCCTGAATATTCTCAACACGGT
AGATGTGAATATCTCCACTGCCGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTTGCCCAGGCGCTTCGAGCGTTCCTGCGCCAGGATCCGGACATCATCATGGTCGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGTGCTGC
GGAAACGCTTACGCGCTTGCGCAACATGGGGGTGCCGGCATTCAACATTGCCACATCGGTCAACCTGATCATTGCTCAAC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGCAAGCAAGAAGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGTTTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAGGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAGAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTCAAGGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-148)

• Type II/IV secretion system protein (T2SSE) (191-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	59.964	99.118	0.594
pilB	Acinetobacter baylyi ADP1	58.511	99.471	0.582
pilB	Legionella pneumophila strain ERS1305867	54.991	100	0.554
pilB	Vibrio cholerae strain A1552	51.636	97.002	0.501
pilB	Vibrio parahaemolyticus RIMD 2210633	51.099	96.296	0.492
pilB	Vibrio campbellii strain DS40M4	50.733	96.296	0.489
pilF	Neisseria gonorrhoeae MS11	50.645	95.767	0.485
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.566	93.474	0.379
pilF	Thermus thermophilus HB27	38.188	99.295	0.379