Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LDN16_RS07145 Genome accession   NZ_AP024961
Coordinates   1598267..1599991 (+) Length   574 a.a.
NCBI ID   WP_027265235.1    Uniprot ID   -
Organism   Legionella pneumophila strain 80-045     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1593267..1604991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDN16_RS07125 (LEG80045_13820) - 1593620..1596142 (+) 2523 WP_223919161.1 GGDEF domain-containing phosphodiesterase -
  LDN16_RS07130 (LEG80045_13830) - 1596144..1596713 (-) 570 WP_223919162.1 hypoxanthine-guanine phosphoribosyltransferase -
  LDN16_RS07135 (LEG80045_13840) - 1596756..1597082 (-) 327 WP_011213802.1 DUF1820 family protein -
  LDN16_RS07140 - 1597265..1598008 (-) 744 WP_027265234.1 methyltransferase domain-containing protein -
  LDN16_RS07145 (LEG80045_13850) pilB 1598267..1599991 (+) 1725 WP_027265235.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LDN16_RS07150 (LEG80045_13860) pilC 1599997..1601217 (+) 1221 WP_027265236.1 type II secretion system F family protein Machinery gene
  LDN16_RS07155 (LEG80045_13870) pilD 1601263..1602126 (+) 864 WP_027265237.1 A24 family peptidase Machinery gene
  LDN16_RS07160 (LEG80045_13880) - 1602140..1603345 (-) 1206 WP_027265238.1 cyclic nucleotide-binding domain-containing protein -
  LDN16_RS07165 (LEG80045_13890) - 1603453..1604046 (-) 594 WP_027265239.1 hypothetical protein -
  LDN16_RS07170 (LEG80045_13900) - 1604062..1604517 (-) 456 WP_010947256.1 hypothetical protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62688.18 Da        Isoelectric Point: 5.5861

>NTDB_id=76080 LDN16_RS07145 WP_027265235.1 1598267..1599991(+) (pilB) [Legionella pneumophila strain 80-045]
MALATEEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKMSLLQYIVKNKILSAEQIALTAAQNFGVPMLDINCIDVGTI
PANLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSALIDNLLTAKESQGLSEYVE
DSGDLEGLEISADDEDQDSDTATSVTDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLS
SRITARIKVMSNLDISERRIPQDGGFKMKISKSRAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRTNFLK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKESDLVNLKLYKAVGCEQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=76080 LDN16_RS07145 WP_027265235.1 1598267..1599991(+) (pilB) [Legionella pneumophila strain 80-045]
ATGGCTCTAGCTACAGAAGAATATAGATTACAGGGAATCGGCCAGCTTCTCGTGCTGGAAAAGCTGCTAGATAAAACAAA
AGCAATTGAACTACACAAGCTGGCAGCAGCAGAAAAGATGTCTTTGCTGCAATACATTGTAAAAAATAAAATATTATCTG
CTGAACAAATTGCACTGACAGCTGCTCAAAATTTTGGCGTACCCATGTTGGATATTAACTGCATTGATGTAGGCACCATT
CCTGCCAATCTGGTTAATGAGAAATTAATTAAACGTCATGCCATGGTTCCTCTTTTTAGCCGCGGTACCAATTTATATCT
CGCAACAGATGATCCTAGTAAACAAGCTTCATTAAAGGAAATTCAATTTCACACCGGATTAAATACTCATGCGATAGTTG
TAGAAACAGATAAACTTAGTGCCCTGATTGATAACCTGTTAACAGCAAAGGAAAGTCAGGGCTTATCAGAGTATGTTGAA
GACTCTGGAGATCTGGAAGGTTTAGAAATAAGTGCTGATGATGAAGATCAAGATAGTGATACTGCAACATCAGTAACTGA
TGATGCACCTATCGTAAAATTCGTCAATAAAATTTTATTGGATGCGATAAGGCAAGGAGCTTCTGATATACACTTTGAGC
CTTATGAACGAGAATACCGAATCAGATACAGACAAGATGGTATTTTACATGAAGTCGCTACCCCTCCCGCAAGCTTGTCA
TCTCGTATTACAGCACGTATCAAGGTAATGTCTAATTTGGATATATCAGAACGGCGTATCCCCCAGGATGGTGGCTTCAA
AATGAAAATTTCCAAGTCACGAGCAATTGATTTCAGAGTGAGTACATGTCCAACTTCGGCTGGGGAAAAAGTGGTTATGC
GGGTTCTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTGGGATTTAATCCTGTTCAGCGAACAAATTTCCTGAAA
GCAATCCAGCGCCCACAAGGGATGATTTTAGTCACTGGCCCTACTGGTAGTGGTAAAACAGTGACTTTATATACGGCTTT
AAATATATTAAATACAATTGAGGTTAATATTTCAACAGCTGAAGATCCAGTTGAAATCAAAGTGCCTGGTATCAATCAGG
TTAATATTAATCCTAAAGCGGGCCTAACCTTTTCCGGTGCATTACGTTCGTTTCTAAGGCAAGACCCTGATATTATAATG
GTTGGTGAGATACGAGACCTTGAAACAGCAGAGATTGCTGTAAAAGCTGCTCAGACAGGGCATTTGGTTCTTTCTACTCT
GCACACAAACAGTGCCGCTGAAACTTTAAATCGTTTAGTAAACATGGGAATACCAACTTTTAACATTGCGAGTTCGGTTA
CCTTAATTATTGCGCAAAGGCTGGCAAGAAAATTATGCAATCAATGTAAGGCAGTGCGAGATGACTTTACCAATCAAGGC
TTGATTGAACTAGGTTTCAAGGAATCTGATCTGGTTAATCTTAAATTATACAAAGCAGTTGGTTGCGAGCAATGTACAAG
TGGTTATCGCGGACGAGTCGGGTTGTTTGAGGTTTTACCGATGACGAAAGAGCTTGGCCAATTGATTATGTCAGGTGGTA
ATTCGCTGGATATATTAAAGTTAGCTCAATCAGAAGGTATGCTGACTATTTTTCAATCGGGTATAGAAAAAGTTAAGGAA
GGAATCACGACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-151)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

99.129

100

0.991
  pilB Acinetobacter baumannii D1279779

50.617

98.78

0.5
  pilB Acinetobacter baylyi ADP1

49.22

100

0.495
  pilB Vibrio cholerae strain A1552

50.741

94.077

0.477
  pilF Neisseria gonorrhoeae MS11

46.996

98.606

0.463
  pilB Vibrio campbellii strain DS40M4

47.217

97.038

0.458
  pilB Vibrio parahaemolyticus RIMD 2210633

48.609

93.902

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.811

92.334

0.368
  pilF Thermus thermophilus HB27

38.476

93.728

0.361

Multiple sequence alignment