Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   P7I92_RS23020 Genome accession   NZ_CP124654
Coordinates   4930748..4932157 (-) Length   469 a.a.
NCBI ID   WP_282415079.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01851     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4925748..4937157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I92_RS23005 (P7I92_23005) - 4926716..4927828 (-) 1113 WP_058355125.1 PstS family phosphate ABC transporter substrate-binding protein -
  P7I92_RS23010 (P7I92_23010) lapA 4927914..4929020 (-) 1107 WP_003116534.1 alkaline phosphatase LapA -
  P7I92_RS23015 (P7I92_23015) pilC 4929534..4930748 (-) 1215 WP_003141142.1 type II secretion system inner membrane protein GspF Machinery gene
  P7I92_RS23020 (P7I92_23020) pilB 4930748..4932157 (-) 1410 WP_282415079.1 type II secretion system ATPase GspE Machinery gene
  P7I92_RS23025 (P7I92_23025) gspD 4932154..4934565 (-) 2412 WP_282415080.1 type II secretion system secretin GspD -
  P7I92_RS23030 (P7I92_23030) gspM 4934562..4935155 (-) 594 WP_003112724.1 type II secretion system protein GspM -
  P7I92_RS23035 (P7I92_23035) gspL 4935142..4936353 (-) 1212 WP_031638419.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 51022.46 Da        Isoelectric Point: 5.7943

>NTDB_id=759875 P7I92_RS23020 WP_282415079.1 4930748..4932157(-) (pilB) [Pseudomonas aeruginosa strain 2021CK-01851]
MSLLPYAWAKAQRALLRPGEHGATLLVSPRTPGWAISEVRQRHALASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLMDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLRLETLGMAPGVLAPLDNLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRVEEDGGWRAVGCPVCNQ
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGSTTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=759875 P7I92_RS23020 WP_282415079.1 4930748..4932157(-) (pilB) [Pseudomonas aeruginosa strain 2021CK-01851]
ATGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCTTGCTGCGGCCGGGCGAGCATGGCGCGACCCTGCTGGT
CAGCCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCGCTGGCCAGCCTGGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTACTCCGATACCGGCAGCGCCGCGGCGGTGGTCGGCGCGGCGGAGAGCGAG
GTCGACCTCGACCGCCTGATGGACGATATCCCCGAGGTCACCGACCTGCTCGACACCCAGGATGGCGCGCCAGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGATGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTGTCGCGG
ATCAAGATCATGGCGCAGCTGGATATCGCCGAGAAACGCCTGCCCCAGGATGGCCGCATCGCCTTGCGGGTAGCCGGGCG
GCCGATCGACATTCGCGTCTCCACCGTGCCCACCGGCCACGGCGAACGGGTGGTGATGCGCCTGCTCGACAAGCAGGCCG
GGCGCCTGAGGCTGGAGACCCTGGGCATGGCTCCCGGCGTGCTGGCGCCGCTGGACAACCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACTACCCTCTACGCCGCCCTGGCGCGGCTCGATGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCGGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTAGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGACACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACCCGGCTGGTGGACATGGGTGTCGAGCCGTTCCTGCTCGCCTCGTCGATGCTTGGCGTACTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGGTCGAAGAGGACGGCGGCTGGCGCGCGGTTGGCTGCCCTGTCTGCAACCAG
ACCGGCTACAGTGGGCGCACCGGCATCCACGAACTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCTCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilF Thermus thermophilus HB27

50.928

80.384

0.409
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.403

94.243

0.39
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.076

84.222

0.388
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.103

82.729

0.373