Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ORU25_RS06525 Genome accession   NZ_CP111145
Coordinates   1302240..1303625 (+) Length   461 a.a.
NCBI ID   WP_010906472.1    Uniprot ID   Q9CPF8
Organism   Pasteurella multocida strain PF9     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1297240..1308625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ORU25_RS06495 (ORU25_06495) - 1297432..1298283 (-) 852 WP_010906470.1 thymidylate synthase -
  ORU25_RS06500 (ORU25_06500) lgt 1298280..1299092 (-) 813 WP_010906471.1 prolipoprotein diacylglyceryl transferase -
  ORU25_RS06505 (ORU25_06505) - 1299102..1299896 (-) 795 WP_005722415.1 sulfite exporter TauE/SafE family protein -
  ORU25_RS06510 (ORU25_06510) rppH 1299899..1300495 (-) 597 WP_005722417.1 RNA pyrophosphohydrolase -
  ORU25_RS06515 (ORU25_06515) ampD 1301080..1301646 (-) 567 WP_005751074.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ORU25_RS06520 (ORU25_06520) pilA 1301806..1302240 (+) 435 WP_005741096.1 prepilin peptidase-dependent pilin Machinery gene
  ORU25_RS06525 (ORU25_06525) pilB 1302240..1303625 (+) 1386 WP_010906472.1 GspE/PulE family protein Machinery gene
  ORU25_RS06530 (ORU25_06530) - 1303625..1304836 (+) 1212 Protein_1267 type II secretion system F family protein -
  ORU25_RS06535 (ORU25_06535) - 1304836..1305558 (+) 723 WP_010906474.1 prepilin peptidase -
  ORU25_RS06540 (ORU25_06540) coaE 1305573..1306193 (+) 621 WP_010906475.1 dephospho-CoA kinase -
  ORU25_RS06545 (ORU25_06545) yacG 1306204..1306407 (+) 204 WP_005719879.1 DNA gyrase inhibitor YacG -
  ORU25_RS06550 (ORU25_06550) - 1306407..1306676 (+) 270 WP_005722568.1 GNAT family N-acetyltransferase -
  ORU25_RS06555 (ORU25_06555) - 1307125..1308498 (+) 1374 WP_005722571.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52078.72 Da        Isoelectric Point: 6.3686

>NTDB_id=758163 ORU25_RS06525 WP_010906472.1 1302240..1303625(+) (pilB) [Pasteurella multocida strain PF9]
MFHENPLCTVSAMTGEVFEIQQALWQKNQQQQRFLLRYFALPLQEDEKQLWLGVDSLANLSACDAFSFLTNKHIEPVLLS
TNELKRALQQLSPPKLQVEENIPTVYQVAQLDSDSATDEPLIQLLDQVFERALQLQASDIHFEPQQHSLQVRFRIDGVLQ
VQPSFSGILANRLISRLKLLAKLDISETRLPQDGCFQFKTTFSDTLDFRLSTLPTHLGEKAVLRLQRNKPIHLSFGELGM
TADQQHRFQHALSQPQGLILVTGPTGSGKSISLYSALAWLNSIEKHILTAEDPIEIALTGIIQTQVNTQIGLDFKRLLRT
FLRQDPDIIMLGEIRDEESALIALRAAQTGHLVLSTLHTNDAPSALTRLQQLGVPLHEIEQSLLLVVAQRLLRRCCDCRV
ETQSACRCYQGYKGRIGVYQFLQKDKTGCFVTDFSSLRESAKEKVRLGMTDDNEVQRVLGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=758163 ORU25_RS06525 WP_010906472.1 1302240..1303625(+) (pilB) [Pasteurella multocida strain PF9]
ATGTTTCATGAGAATCCTTTGTGTACTGTTAGCGCAATGACAGGAGAGGTATTTGAAATCCAACAGGCGCTATGGCAAAA
AAATCAGCAACAACAACGTTTTTTATTACGTTATTTTGCCTTGCCTTTACAAGAAGATGAAAAACAGTTGTGGCTTGGTG
TGGATTCGTTAGCGAATTTATCAGCATGTGATGCCTTTTCTTTTTTAACGAATAAACACATTGAGCCTGTTTTATTATCA
ACAAATGAACTTAAACGGGCATTGCAGCAACTTTCCCCACCAAAATTACAGGTGGAAGAAAACATACCCACGGTTTATCA
AGTCGCGCAATTAGATTCTGATTCCGCGACGGATGAACCTTTGATTCAATTACTTGATCAGGTTTTTGAACGTGCATTAC
AGCTACAGGCTTCTGACATTCATTTTGAACCGCAACAGCATAGCTTACAAGTGCGTTTTAGAATCGATGGTGTATTACAA
GTCCAGCCTTCCTTTTCAGGGATATTGGCTAATCGCCTGATTTCTCGTCTTAAATTATTAGCAAAATTAGATATTAGTGA
AACACGCTTGCCTCAGGACGGTTGTTTCCAATTTAAAACGACATTTTCTGATACATTAGATTTTCGACTTTCTACGTTGC
CGACACATTTAGGCGAAAAAGCGGTATTGCGTTTACAGCGAAATAAGCCTATTCATCTCAGTTTTGGTGAGTTGGGGATG
ACCGCAGACCAACAACACAGATTCCAACACGCGTTAAGTCAGCCACAAGGACTGATTTTAGTAACGGGACCAACAGGGAG
TGGAAAAAGCATTTCGCTTTATAGTGCGCTAGCTTGGCTCAACAGCATAGAAAAGCATATTTTAACAGCAGAAGATCCGA
TTGAAATTGCGTTGACTGGGATAATCCAAACGCAAGTAAATACGCAAATAGGACTGGATTTCAAGCGTTTATTACGCACT
TTTTTACGTCAAGATCCAGACATTATTATGTTAGGGGAAATTCGTGATGAAGAAAGCGCGTTAATCGCATTACGTGCAGC
TCAAACTGGGCATTTAGTCCTCTCTACCTTGCATACGAATGATGCGCCCTCTGCCCTTACTCGCTTACAGCAATTAGGGG
TGCCTTTGCATGAAATTGAACAAAGCCTCTTACTTGTTGTGGCGCAGCGTTTATTGCGTCGTTGTTGTGATTGCCGTGTA
GAGACACAATCGGCGTGTCGTTGCTATCAGGGGTATAAAGGGAGGATTGGGGTATATCAATTTTTACAAAAAGATAAAAC
AGGGTGTTTTGTCACGGATTTTAGTAGTTTACGCGAGAGTGCGAAGGAAAAAGTTCGTTTAGGCATGACCGATGACAATG
AAGTACAACGTGTATTAGGTGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-93)

Type II/IV secretion system protein (T2SSE)

(128-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CPF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

67.991

98.265

0.668
  pilB Haemophilus influenzae Rd KW20

67.55

98.265

0.664
  pilB Glaesserella parasuis strain SC1401

58.811

98.482

0.579
  pilB Acinetobacter baumannii D1279779

39.757

100

0.425
  pilB Vibrio campbellii strain DS40M4

39.419

100

0.412
  pilB Acinetobacter baylyi ADP1

37.876

100

0.41
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio cholerae strain A1552

38.272

100

0.403
  pilB Legionella pneumophila strain ERS1305867

37.219

100

0.395