NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	ORU08_RS06615	Genome accession	NZ_CP111142
Coordinates	1376668..1377102 (-)	Length	144 a.a.
NCBI ID	WP_005741096.1	Uniprot ID	-
Organism	Pasteurella multocida strain PF5
Function	type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1371668..1382102

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ORU08_RS06585 (ORU08_06585)	-	1372232..1372501 (-)	270	WP_005722568.1	GNAT family N-acetyltransferase	-
ORU08_RS06590 (ORU08_06590)	yacG	1372501..1372704 (-)	204	WP_005719879.1	DNA gyrase inhibitor YacG	-
ORU08_RS06595 (ORU08_06595)	coaE	1372715..1373335 (-)	621	WP_010906475.1	dephospho-CoA kinase	-
ORU08_RS06600 (ORU08_06600)	-	1373350..1374072 (-)	723	WP_010906474.1	prepilin peptidase	-
ORU08_RS06605 (ORU08_06605)	-	1374072..1375283 (-)	1212	WP_005722560.1	type II secretion system F family protein	-
ORU08_RS06610 (ORU08_06610)	pilB	1375283..1376668 (-)	1386	WP_078801766.1	GspE/PulE family protein	Machinery gene
ORU08_RS06615 (ORU08_06615)	pilA	1376668..1377102 (-)	435	WP_005741096.1	prepilin peptidase-dependent pilin	Machinery gene
ORU08_RS06620 (ORU08_06620)	ampD	1377262..1377828 (+)	567	WP_225529746.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
ORU08_RS06625 (ORU08_06625)	rppH	1378413..1379009 (+)	597	WP_005722417.1	RNA pyrophosphohydrolase	-
ORU08_RS06630 (ORU08_06630)	-	1379012..1379806 (+)	795	WP_005722415.1	sulfite exporter TauE/SafE family protein	-
ORU08_RS06635 (ORU08_06635)	lgt	1379816..1380628 (+)	813	WP_010906471.1	prolipoprotein diacylglyceryl transferase	-
ORU08_RS06640 (ORU08_06640)	-	1380625..1381476 (+)	852	WP_010906470.1	thymidylate synthase	-
ORU08_RS06645 (ORU08_06645)	tadA	1381491..1381991 (+)	501	WP_005755875.1	tRNA adenosine(34) deaminase TadA	-

Sequence

Protein

Download Length: 144 a.a. Molecular weight: 15058.48 Da Isoelectric Point: 9.1281

                                >NTDB_id=758081 ORU08_RS06615 WP_005741096.1 1376668..1377102(-) (pilA) [Pasteurella multocida strain PF5]

MKKAIFFSIKKGFTLIELMIVIAIIAILATIAVPSYQNYTKKAAISELLQAAAPYRAEVELCIYNTNSTTNCNAGSHGIR

ADAAASKKYLKAITVKAGVITVTGQGSLEGISYTLTASGNAAQGVSWTVNCGAQSDIFPAGFCA

Nucleotide

Download Length: 435 bp

                                >NTDB_id=758081 ORU08_RS06615 WP_005741096.1 1376668..1377102(-) (pilA) [Pasteurella multocida strain PF5]

ATGAAAAAAGCCATTTTCTTTTCGATTAAAAAAGGGTTTACGCTAATTGAATTAATGATTGTCATTGCCATTATTGCGAT

TTTAGCCACGATTGCCGTGCCATCTTATCAAAATTATACAAAAAAAGCGGCAATCTCTGAATTATTGCAAGCCGCTGCTC

CTTATCGTGCTGAGGTAGAACTTTGTATCTATAATACAAATAGTACAACGAATTGTAATGCAGGAAGTCATGGTATTCGT

GCTGATGCTGCAGCAAGTAAAAAATATTTAAAAGCCATCACAGTTAAAGCTGGGGTAATTACTGTCACTGGACAAGGGAG

CTTGGAAGGAATTAGCTATACGCTGACAGCGAGTGGTAACGCAGCTCAAGGTGTTTCTTGGACAGTTAATTGTGGTGCTC

AATCTGACATTTTCCCAGCAGGATTTTGCGCATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Haemophilus influenzae 86-028NP	63.194	100	0.632
pilA	Haemophilus influenzae Rd KW20	61.111	100	0.611
pilA	Glaesserella parasuis strain SC1401	52.349	100	0.542
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.368
pilA	Vibrio cholerae strain A1552	36.806	100	0.368
pilA	Vibrio cholerae C6706	36.806	100	0.368

Detailed information NTG0758081